Connexin 43 expression in normal versus dysfunctional labor

Pierce, Brian; Calhoun, Byron C.; Adolphson, Kimberly R.; Lau, Alan F.; Pierce, Lisa

doi:10.1067/mob.2002.121108

Cited by 15 publications

(13 citation statements)

References 22 publications

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“…Animal data consistently show that both mRNA and protein levels of CX-43 increase with the onset of labour (Lye 1993, McNutt et al 1994, Ou et al 1997, Cook et al 2000 and that ablation of CX-43 delays the onset of parturition (Doring et al 2006). Human data are less robust, with some studies showing increases in protein alone (Sparey et al 1999), some in mRNA but not protein (Chow & Lye 1994, Pierce et al 2002 and some no change in mRNA (Rezapour et al 1997). The spatial expression of CX-43 protein was assessed in one study and found to be greater in the US in both non-labour and labour samples (Sparey et al 1999).…”

Section: Discussionmentioning

confidence: 99%

“…The spatial expression of CX-43 protein was assessed in one study and found to be greater in the US in both non-labour and labour samples (Sparey et al 1999). Two studies suggested that failure to progress in labour was associated with lower levels of CX-43 mRNA expression (Pierce et al 2002, Cluff et al 2006. We found that CX-43 mRNA expression, protein levels and phosphorylation status were unchanged with either term or PTL.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Tattersall¹,

Engineer²,

Khanjani³

et al. 2008

Reproduction

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Preterm labour (PTL) is the most important cause of neonatal morbidity and mortality. While some causes have been identified, the mechanisms involved remain elusive. This study investigates whether term labour (TL) is an appropriate model for PTL by examining prolabour gene expression, using quantitative rtPCR, and protein synthesis, using Western analysis, in preterm and term myometrial samples obtained from the upper and lower uterine segments before and after the onset of labour. In the lower segment, the levels of prostaglandin H synthase type-2 (PGHS-2), interleukin-1b (IL-1b), IL-6 and IL-8 mRNA expression were significantly higher in TL compared with PTL samples. Compared with non-labour controls, the expression of IL-1b and IL-8 mRNA was increased in both PTL and TL samples and the expression of PGHS-2 and IL-6 mRNA was increased in TL samples only. In the upper segment, there were no differences between PTL and TL samples and the mRNA expression of PGHS-2 and IL-1b was increased in TL compared with term no labour samples. No effect of PTL or TL was seen on either oxytocin receptor or connexin-43 mRNA expression or protein levels. The multiple regression analysis and studies in primary cultures of uterine myocytes suggest that the inflammatory cytokines, IL-1b and tumour necrosis factor-a, are the most important regulators of PGHS-2 and IL-8. Our data show that preterm and term labouring myometrium are significantly different and that the most marked labour-induced changes in gene expression are in the lower segment. These changes may occur in response to the release of inflammatory cytokines by the labour-associated inflammatory infiltration. Reproduction (2008) 135 569-579

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Tattersall¹,

Engineer²,

Khanjani³

et al. 2008

Reproduction

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“…] i dynamics The gap junctions in epithelia and other cells have a very important role in permitting elements to flux from one cell to an adjacent cell, thus, mediating cellular communication (Höfer et al, 1999;Nielsen et al, 2002;Pierce et al, 2002;Yao et al, 2002;Sáez et al, 2003;Fanchaouy et al, 2005). To consider the causes of the spontaneous changes of [Ca 2+ ] i in young and adult animals we focused on the differences of connexin proteins in the two animal groups.…”

Section: +mentioning

confidence: 99%

“…The results indicate that gap junctions play an important role in the communication between epithelial cells or other tissue cells (Jara et al, 1995;Çiray et al, 2000;Oyamada et al, 2002;Nielsen et al, 2002;Pierce et al, 2002;Fanchaouy et al, 2005). Gap junctions consist of a family of connexin proteins (such as Cx26, Cx32 and Cx43), which form the channels that permit the passage of ions from cell to cell.…”

Section: Introductionmentioning

confidence: 99%

The morphological and functional observation of the gap junction proteins in the oviduct epithelia in young and adult hamsters

Yan

Akutsu

Satoh

2011

Okajimas Folia Anat. Jpn.

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“…1 Coordinating the contractions of large numbers of myocytes within a syncytium seems to be necessary to mimic the phasic nature of the contractile activity that is important for labor. It is well known that cell-to-cell communication through gap junctions is necessary for tissuelevel coordination, 2 and it is likely that the structure imposed by the scaffold is also a key component.…”

Section: Introductionmentioning

confidence: 99%

Allo- and Xeno-Reassembly of Human and Rat Myometrium from Cells and Scaffolds

Young

Goloman

2013

Tissue Engineering Part A

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Neo-myometrium was created by culturing isolated myocytes into decellularized rat and human myometrial scaffolds. The dual purpose of the uterus is to accommodate the growing fetus, and then expel the fetus at term by phasically contracting it. The first function requires physical robustness as well as the ability to expand and remodel. Congenital anomalies or previous surgeries can mechanically compromise the uterus and lead to major complications in pregnancy. The second function utilizes multiple interactions of complex physiological mechanisms that have yet to be fully elucidated, and this knowledge gap contributes to the continuation of serious complications of pregnancy. To address both problems, we reconstructed myometrium from isolated myocytes and scaffold. From both rat and human myometrium, myocytes were isolated using collagenase digestion, and scaffolds were isolated using ethanol/ trypsin protocols. The number of myocytes was amplified using monolayer culture, and then, the myocytes were cultured back into the scaffolds. We called this engineered tissue neo-myometrium, with allo-neo-myometrium being made from components of the same species, and xeno-neo-myometrium from across species. By artificially creating defects in rat scaffold, alloneo-myometrium was created that demonstrated rapid scaffold remodeling. Xeno-neo myometrium (human myocytes/rat scaffold) was created and demonstrated myocytes occurring in bundles 500 mm deep in the scaffold. These experiments provide proof of principle that modest numbers of myocytes can be amplified and used to create a larger volume of engineered tissue, which may be useful for semi-autologous transplantation to repair structural defects of the human uterus. In isometric contractility experiments, coordinated contractions were observed in xeno-neo-myometrium (human myocytes, rat scaffold), but not allo-neo-myometrium (human myocytes, human scaffold).

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Connexin 43 expression in normal versus dysfunctional labor

Cited by 15 publications

References 22 publications

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

Pro-labour myometrial gene expression: are preterm labour and term labour the same?

The morphological and functional observation of the gap junction proteins in the oviduct epithelia in young and adult hamsters

Allo- and Xeno-Reassembly of Human and Rat Myometrium from Cells and Scaffolds

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