Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common
            <i>Neisseria meningitidis</i>
            Serogroups Associated with Invasive Disease

Bennett, Désirée E.; Cafferkey, Mary T.

doi:10.1128/jcm.44.3.1127-1131.2006

Cited by 27 publications

(24 citation statements)

References 35 publications

(47 reference statements)

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“…meningitidis isolate which, together with the N. meningitidis isolates cultured from carriage ( n = 14), was further characterized by serotyping (20), genogrouping (21), and multilocus restriction typing (MLRT) (22). …”

Section: Methodsmentioning

confidence: 99%

Resolution of a Protracted Serogroup B Meningococcal Outbreak with Whole-Genome Sequencing Shows Interspecies Genetic Transfer

Mulhall

Brehony

O’Connor³

et al. 2016

J Clin Microbiol

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A carriage study was undertaken (n = 112) to ascertain the prevalence of Neisseria spp. following the eighth case of invasive meningococcal disease in young children (5 to 46 months) and members of a large extended indigenous ethnic minority Traveller family (n = 123), typically associated with high-occupancy living conditions. Nested multilocus sequence typing (MLST) was employed for case specimen extracts. Isolates were genome sequenced and then were assembled de novo and deposited into the Bacterial Isolate Genome Sequencing Database (BIGSdb). This facilitated an expanded MLST approach utilizing large numbers of loci for isolate characterization and discrimination. A rare sequence type, ST-6697, predominated in disease specimens and isolates that were carried (n = 8/14), persisting for at least 44 months, likely driven by the high population density of houses (n = 67/112) and trailers (n = 45/112). Carriage for Neisseria meningitidis (P < 0.05) and Neisseria lactamica (P < 0.002) (2-sided Fisher's exact test) was more likely in the smaller, more densely populated trailers. Meningococcal carriage was highest in 24- to 39-year-olds (45%, n = 9/20). Evidence of horizontal gene transfer (HGT) was observed in four individuals cocolonized by Neisseria lactamica and Neisseria meningitidis. One HGT event resulted in the acquisition of 26 consecutive N. lactamica alleles. This study demonstrates how housing density can drive meningococcal transmission and carriage, which likely facilitated the persistence of ST-6697 and prolonged the outbreak. Whole-genome MLST effectively distinguished between highly similar outbreak strain isolates, including those isolated from person-to-person transmission, and also highlighted how a few HGT events can distort the true phylogenetic relationship between highly similar clonal isolates.

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Section: Methodsmentioning

confidence: 99%

Resolution of a Protracted Serogroup B Meningococcal Outbreak with Whole-Genome Sequencing Shows Interspecies Genetic Transfer

Mulhall

Brehony

O’Connor³

et al. 2016

J Clin Microbiol

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“…For example, in the European Union, 25% of cases are confirmed by PCR alone, and in the UK and Ireland, this rises to ~50% [20]; while in the United States, the CDC only recently included PCR as confirmatory in its case definition [6]. There is as yet no international consensus on their use, although PCR methods incorporating a number of targets have been developed (such as ctrA, porA, crgA , 16S rDNA, sodC and cnl ) [21–25]. …”

Section: Current Diagnosis Confirmation and Characterization Of Invamentioning

confidence: 99%

Global Meningococcal Initiative: guidelines for diagnosis and confirmation of invasive meningococcal disease

et al. 2016

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SUMMARYThe Global Meningococcal Initiative (GMI) is an international group of scientists and clinicians with recognized expertise in meningococcal disease including microbiology, immunology, epidemiology, public health and vaccinology. The GMI was established to promote the global prevention of meningococcal disease through education, research and international cooperation. The GMI held its second summit meeting in 2013 to discuss the different aspects of existing meningococcal immunization programmes and surveillance systems. Laboratory confirmation and characterization were identified as essential for informing evidence-based vaccine implementation decisions. The relative merits of different confirmatory methodologies and their applications in different resource settings were a key component of the discussions. This paper summarizes the salient issues discussed, with special emphasis on the recommendations made and any deficiencies that were identified.

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“…Characterisation of N. meningitidis isolates using serotyping, genogrouping, porA/fetA finetyping, multilocus restriction typing (MLRT) and MLST was also undertaken [13][14][15][16][17][18]. Antimicrobial susceptibility testing was performed on isolates as above.…”

Section: Carriage Studymentioning

confidence: 99%

A prolonged outbreak of invasive meningococcal disease in an extended Irish Traveller family across three Health Service Executive (HSE) areas in Ireland, 2010 to 2013

O’Connor¹,

Ward²,

Bennett

et al. 2015

Eurosurveillance

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Consecutive Use of Two Multiplex PCR-Based Assays for Simultaneous Identification and Determination of Capsular Status of Nine Common Neisseria meningitidis Serogroups Associated with Invasive Disease

Cited by 27 publications

References 35 publications

Resolution of a Protracted Serogroup B Meningococcal Outbreak with Whole-Genome Sequencing Shows Interspecies Genetic Transfer

Resolution of a Protracted Serogroup B Meningococcal Outbreak with Whole-Genome Sequencing Shows Interspecies Genetic Transfer

Global Meningococcal Initiative: guidelines for diagnosis and confirmation of invasive meningococcal disease

A prolonged outbreak of invasive meningococcal disease in an extended Irish Traveller family across three Health Service Executive (HSE) areas in Ireland, 2010 to 2013

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