Consensus RT-nested PCR detection of yellow head complex genotypes in penaeid shrimp

Wijegoonawardane, Priyanjalie K.M.; Cowley, Jeff A.; Walker, Peter

doi:10.1016/j.jviromet.2008.07.009

Cited by 27 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to other quantification methods, the Loopamp turbidimeter is comparatively less expensive than Q-PCR, which requires fluorogenic primers and probes [16]. The most beneficial property of the V. nigripulchritudo Q-LAMP assay is its rapidity; detection can be completed within a short duration, whereas conventional PCR methods require 2-3 h [37].…”

Section: Discussionmentioning

confidence: 99%

Quantitative loop-mediated isothermal amplification method for the detection of Vibrio nigripulchritudo in shrimp

et al. 2010

View full text Add to dashboard Cite

Vibrio nigripulchritudo is considered one of the major pathogens threatening shrimp aquaculture. In this study, we developed a novel and highly specific quantitative loop-mediated isothermal amplification (Q-LAMP) assay. A set of four specific primers were designed targeting the V. nigripulchritudo intergenic spacer region. The reaction time and temperature were optimized for 60 min at 63°C. Quantitative analysis was then performed by measuring the turbidity of the reaction solution using a real-time turbidimeter, allowing for quantification of the initial DNA concentration with a sensitivity of 10 2 copy numbers equivalent to 2.3 colony forming units/ml or 0.3 fg/ll. The LAMP assay was able to specifically detect two representative strains of V. nigripulchritudo, whereas other Vibrio and non-Vibrio species were not amplified. A standard curve was generated for V. nigripulchritudo by plotting the threshold time (T t ) versus the log of bacterial number. A high correlation coefficient (R 2 = 0.9749) was observed for the Q-LAMP reaction. In conclusion, Q-LAMP assay is a sensitive, rapid, and simple tool that can be used for the detection and quantification of V. nigripulchritudo in shrimp, thereby facilitating surveillance of vibriosis infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Quantitative loop-mediated isothermal amplification method for the detection of Vibrio nigripulchritudo in shrimp

et al. 2010

View full text Add to dashboard Cite

show abstract

“…As several geographical types of YHV have been reported (Wijegoonawardane 2008; Wijegoonawardane et al. 2008a,b), it was of epidemiological interest to know which type of YHV was responsible for the disease outbreaks.…”

Section: Resultsmentioning

confidence: 99%

“…So far, there have been four reports on four YHV isolates with a deletion sequence in ORF3 (Wijegoonawardane 2008; Wijegoonawardane et al. 2008a,b; Gangnonngiw et al.…”

Section: Resultsmentioning

confidence: 99%

“…By the same token, some are serious pathogens for both species, and among these, the RNA virus yellow head virus (YHV) is one of the most important. Since the first report of YHV in Thailand in 1990 (Limsuwan 1991), six geographical types have been described (YHV‐1 to YHV‐6) (Wijegoonawardane 2008; Wijegoonawardane, Cowley & Walker 2008a; Wijegoonawardane, Cowley, Phan, Hodgson, Nielsen, Kiatpathomchai & Walker 2008b). These types differ in virulence and in overall genome sequences by up to 20% (Cowley, Dimmock, Wongteerasupaya, Boonsaeng, Panyim & Walker 1999; Sittidilokratna, Hodgson, Cowley, Jitrapakdee, Boonsaeng, Panyim & Walker 2002; Wijegoonawardane 2008).…”

Section: Introductionmentioning

confidence: 99%

“…2008). A methodology for designation of 6 YHV genotypes based on regions in ORF1b and ORF3 has been recently presented (Wijegoonawardane 2008; Wijegoonawardane et al. 2008a,b).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of yellow head virus outbreaks in the whiteleg shrimp, Penaeus vannamei (Boone), in Thailand

Senapin

Thaowbut

Gangnonngiw

et al. 2010

Journal of Fish Diseases

View full text Add to dashboard Cite

Yellow head virus (YHV) is known as a major pathogen in the black tiger shrimp, Penaeus (Penaeus) monodon. It can also cause serious mortality in farmed whiteleg shrimp, Penaeus (Litopenaeus) vannamei. However, there is no published information on the economic and/or production impact of the disease in P. vannamei. Shrimp with gross signs of YHV disease (faded body colour and 60-70% mortality) were observed in 20 study farms rearing P. vannamei in the central part of Thailand from the end of 2007 through early 2008. The estimated economic loss for these farms according to the Thai Animal Aquaculture Association was approximately US$3 million. Detailed sequence analysis of RT-PCR amplicons from shrimp in all the study ponds revealed the presence of YHV Type 1b (YHV-1b) alone (characterized by a 162-bp deletion in the ORF3 region encoding the structural gene for gp116) and the absence of YHV Type 1a (YHV-1a), the original YHV type reported from Thailand. Despite the large 162-bp deletion (= 54 deduced amino acids) in the gp116 structural gene, histopathology of YHV-1b infections was identical to that of YHV-1a infections, and electron microscopy revealed that YHV-1b virions were morphologically indistinguishable from those previously reported for YHV-1a. In addition, an existing commercial RT-PCR detection kit and an immunochromatographic test strip for the detection of YHV were proven to have been valid tests for both YHV-1b and YHV-1a. The source of the virus for these outbreaks was unlikely to have been the post-larvae used to stock the ponds, as they were derived from domesticated specific pathogen-free stocks free of YHV. Thus, it is possible that they originated from an unknown, natural reservoir.

show abstract

Diagnosis of Aquatic Animal Viral Diseases

Kibenge

Godoy

Kibenge

2016

Aquaculture Virology

View full text Add to dashboard Cite

Consensus RT-nested PCR detection of yellow head complex genotypes in penaeid shrimp

Cited by 27 publications

References 31 publications

Quantitative loop-mediated isothermal amplification method for the detection of Vibrio nigripulchritudo in shrimp

Quantitative loop-mediated isothermal amplification method for the detection of Vibrio nigripulchritudo in shrimp

Impact of yellow head virus outbreaks in the whiteleg shrimp, Penaeus vannamei (Boone), in Thailand

Diagnosis of Aquatic Animal Viral Diseases

Contact Info

Product

Resources

About