Conserved expression pattern of chicken DAZL in primordial germ cells and germ-line cells

Rengaraj, Deivendran; Zheng, Ying; Kang, Ki Ser; Park, K.J.; Lee, Bo Ram; Lee, S.I.; Choi, Jin Won; Han, Jae Yong

doi:10.1016/j.theriogenology.2010.04.001

Cited by 41 publications

(32 citation statements)

References 46 publications

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“…8A3 and A4). Quantification of the specific primordial germ cell marker DAZL, reflecting the abundance of spermatogonia (Rengaraj et al 2010), was not altered between the two groups (Fig. 8B).…”

Section: Effect Of Metformin On Markers Of Testis Developmentmentioning

confidence: 91%

The insulin sensitiser metformin regulates chicken Sertoli and germ cell populations

Faure¹,

Guibert²,

Alves³

et al. 2016

Reproduction

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Metformin, an insulin sensitiser from the biguanide family of molecules, is used for the treatment of insulin resistance in type 2 diabetes individuals. It increases peripheral glucose uptake and may reduce food intake. Based on the tight link between metabolism and fertility, we investigated the role of metformin on testicular function using in vitro culture of Sertoli cells and seminiferous tubules, complemented by in vivo data obtained following metformin administration to prepubertal chickens. In vitro, metformin treatment reduced Sertoli cell proliferation without inducing apoptosis and morphological changes. The metabolism of Sertoli cells was affected because lactate secretion by Sertoli cells increased approximately twofold and intracellular free ATP was negatively impacted. Two important pathways regulating proliferation and metabolism in Sertoli cells were assayed. Metformin exposure was not associated with an increased phosphorylation of AKT or ERK. There was a 90% reduction in the proportion of proliferating germ cells after a 96-h exposure of seminiferous tubule cultures to metformin. In vivo, 6-week-old chickens treated with metformin for 3 weeks exhibited reduced testicular weight and a 50% decrease in testosterone levels. The expression of a marker of undifferentiated germ cells was unchanged in contrast to the decrease in expression of 'protamine', a marker of differentiated germ cells. In conclusion, these results suggest that metformin affects the testicular energy content and the proliferative ability of Sertoli and germ cells.

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Section: Effect Of Metformin On Markers Of Testis Developmentmentioning

confidence: 91%

The insulin sensitiser metformin regulates chicken Sertoli and germ cell populations

Faure¹,

Guibert²,

Alves³

et al. 2016

Reproduction

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show abstract

“…To make hybridization probes, total RNA from magnetic-activated cell sorting (MACS)-positive PGCs at E6.5 was reverse-transcribed, and the cDNA was amplified using cDAZLspecific primers (fwd: 5-CGT CAA CAA CCT GCC AAG GA-3, and rev: 5-TTC TTT GCT CCC CAG GAA CC-3, product size 540 bp), as described previously [25]. The polymerase chain reaction (PCR) products of the correct size were cloned into pGEM-T vector (Promega, Madison, WI, USA).…”

Section: Whole-mount In Situ Hybridizationmentioning

confidence: 99%

“…Its functions in germ cells have been well studied in vertebrate species, and include meiotic progression [23] and maintaining the pluripotency of germ cells [24]. In chicken, a previous study reported germ cell-specific expression of chicken DAZL from the embryonic to adult stages in chickens [25]. However, there is no report on DAZL expression during germ cell specification.…”

Section: Introductionmentioning

confidence: 99%

DAZLExpression Explains Origin and Central Formation of Primordial Germ Cells in Chickens

Lee

Choi

Lee

et al. 2016

Stem Cells and Development

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The timing and biological events associated with germ cell specification in chickens have not been determined yet. In this study, we report the origin of primordial germ cells (PGCs) and germ plasm dynamics through investigation of the expression of the chicken homolog of deleted in azoospermia-like (cDAZL) gene during germ cell specification. Asymmetric localization of germ plasm in the center of oocytes from preovulatory follicle stages leads to PGCs being formed in the center. During cleavage stages, DAZL expression pattern changes from a subcellular localization to a diffuse form before and after zygotic genome activation. Meanwhile, PGCs exhibit transcriptional active status during their specification. In addition, knockdown studies of cDAZL, which result in reduced proliferation, aberrant gene expression profiles, and PGC apoptosis in vitro, suggest its possible roles for PGC formation in chicken. In conclusion, DAZL expression reveals formation and initial positioning of PGCs in chickens.

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“…This gene family consists in a Y-linked DAZ gene cluster and the autosomal dazl and boule genes, all of which encode germ cellspecific RNA-binding proteins with a common RNArecognition motif and at least one copy of DAZ repeat (Xu et al 2001). The Y-linked DAZ gene cluster is restricted to catarrhine primates (Agulnik et al 1998;Gromoll et al 1999), while dazl homologs are found only in vertebrates, such as zebrafish (Maegawa et al 1999), medaka (Xu et al 2007), rainbow trout (Li et al 2011), gibel carp (Peng et al 2009), Xenopus (Houston et al 1998), axolotl (Johnson et al 2001), chicken (Rengaraj et al 2010), mouse (Cooke et al 1996) and human (Seboun et al 1997), where they are more closely Huan Ye and Chuang-Ju Li contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish

Yue

et al. 2015

Cell Tissue Res

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The gene family DAZ (deleted in Azoospermia), including boule, dazl and DAZ, performs highly conserved functions in germ cell development and fertility across animal phyla. Differential expression patterns have been demonstrated for the family members in invertebrates and vertebrates including fish. Here, we report the identification of boule and dazl and their expression at both RNA and protein levels in developing and mature gonads of Chinese sturgeon (Acipenser sinensis). Firstly, the isolation of the boule and dazl genes in Chinese sturgeon and the observation of the two genes in coelacanth suggest that dazl originated after the divergence of bony fish from cartilaginous fish but before the emergence of the Actinistia. Quantitative real-time PCR and western blot analyses reveal that boule and dazl RNA and proteins are restricted to the testis and ovary. In situ hybridization and fluorescent immunohistochemistry show that the bisexual mitotic and meiotic germ cell expression of dazl RNA and protein is conserved in vertebrates, while Chinese sturgeon boule RNA and protein exhibit mitotic and meiotic expression in the testis, and also likely display mitotic and meiotic expression in female. Moreover, we directly demonstrate for the first time that sturgeon Balbiani body/mitochondrial cloud disperses in the cytoplasm of early developing oocytes and co-localizes with Dazl to some extent. Finally, urbilaterian boule may also have an ancestral function in oogenesis. Taken together, these results provide useful information on the evolution of DAZ family genes, expression patterns and functions in animal reproduction.

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Conserved expression pattern of chicken DAZL in primordial germ cells and germ-line cells

Cited by 41 publications

References 46 publications

The insulin sensitiser metformin regulates chicken Sertoli and germ cell populations

The insulin sensitiser metformin regulates chicken Sertoli and germ cell populations

DAZLExpression Explains Origin and Central Formation of Primordial Germ Cells in Chickens

Differential expression of fertility genes boule and dazl in Chinese sturgeon (Acipenser sinensis), a basal fish

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