Consideration on peak shape in a batch-type chemiluminescence detection cell for capillary electrophoresis

Tsukagoshi, Kazuhiko; Otsuka, Miwa; Shikata, Yukihiro; Nakajima, Riichiro

doi:10.1016/s0021-9673(01)01170-0

Journal of Chromatography A

2001

DOI: 10.1016/s0021-9673(01)01170-0

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Consideration on peak shape in a batch-type chemiluminescence detection cell for capillary electrophoresis

Kazuhiko Tsukagoshi

Miwa Otsuka

Yukihiro Shikata

et al.

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Cited by 10 publications

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“…CL has already been verified to be a highly sensitive detection method in both FIA and HPLC. − Over the past decade, the applicability of CL detection in CE has been clearly demonstrated. Several CL reagents, such as luminol, − acridinium, peroxyoxalate, − and the Ru(II) complex, have been utilized. However, despite increased development and analysis of the CE-CL detection system, some problems remain unsolved.…”

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Batch-Type Chemiluminescence Detection Cell for Sensitization and Simplification of Capillary Electrophoresis

2002

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A simple and convenient chemiluminescence detection cell was designed for capillary electrophoresis. The detection cell easily combined with capillary electrophoresis equipment. Luminol chemiluminescence was adapted for use with the detection cell. Detailed analysis and testing of the system revealed that luminol could be determined over a range of 2.5 x 10(-10)-6.5 x 10(-7) M (correlation coefficient, 0.999), with a detection limit (S/N = 3) of 2.5 x 10(-10) M (7 amol). Furthermore, each component in a mixture of glycine, glycylglycine, and glycylglycylgycine, which were labeled with isoluminol isothiocyanate, was baseline separated and sensitively detected. Moreover, the stacking procedure was applied to postcolumn detection in capillary electrophoresis. When acetonitrile stacking was used under certain conditions in the present system, chemiluminescence intensities of luminol and labeled compounds were about 1 order of magnitude higher than those obtained without stacking. The detection limit for luminol was 1.5 x 10(-11) M (S/N = 3), representing the highest sensitivity of luminol yet reported. Finally, the effect of p-iodophenol as an enhancer of luminol chemiluminescence was examined under weak alkaline conditions. The chemiluminescence intensity of luminol was approximately 2 orders of magnitude higher than that in the unenhanced reaction. A preliminary immunoassay using horseradish peroxidase-labeled anti-mouse IgG was also developed.

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Batch-Type Chemiluminescence Detection Cell for Sensitization and Simplification of Capillary Electrophoresis

2002

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“…Based on know-how obtained through our research of an ordinary CE with a CL detector, [2][3][4] we successfully developed a microchip CE with a CL detector. 5,6 Transition-metal ions and dansyl amino acids were analyzed by means of microchip systems using luminol 5 and peroxyoxalate reagent, 6 respectively.…”

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Small-Sized Capillary Electrophoresis with a Chemiluminescence Detector Equipped with Cross-Intersection for Sample Injection

2002

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

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Consideration on peak shape in a batch-type chemiluminescence detection cell for capillary electrophoresis

Cited by 10 publications

References 10 publications

Batch-Type Chemiluminescence Detection Cell for Sensitization and Simplification of Capillary Electrophoresis

Batch-Type Chemiluminescence Detection Cell for Sensitization and Simplification of Capillary Electrophoresis

Small-Sized Capillary Electrophoresis with a Chemiluminescence Detector Equipped with Cross-Intersection for Sample Injection

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

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