Considérations expérimentales nouvelles sur l’évolution <i>in vitro</i> en milieu liquide des larves d’<i>Echinococcus granulosus</i>

Benex, J

doi:10.1051/parasite/1968435561

Cited by 14 publications

(6 citation statements)

References 2 publications

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“…The vesiculation processes observed in this study have been previously described by other researchers with sheep samples (Smyth, 1962(Smyth, , 1967Smyth et ah, 1966;Benex, 1968;Heath & Osborn, 1976;Casado & Rodriguez-Caabeiro, 1988a, 1989; revised by Arme, 1987, andSmyth, 1990). We have not observed detached exovesicles and microcysts formed from them, although this process has been described by Rogan & Richards (1986) in cultures of equine protoscoleces.…”

Section: Discussionsupporting

confidence: 85%

Echinococcus granulosus: characterization of the Spanish strains using in vitro vesicular development

Gordo

Bandera

1997

J. Helminthol.

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Differences in in vitro vesicular development (microcyst formation) in three Spanish strains of Echinococcus granulosus (sheep–cattle, horse and pig–goat) are reported. Microcyst formation occurred in 19–37 days (sheep strain), 9–18 days (pig strain) and 35–47 days (horse strain). Comparing these results with those from human samples (microcyst formation in 24–38 days), it is possible to consider the sheep strain as the most likely source of human infections in Spain.

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Section: Discussionsupporting

confidence: 85%

Echinococcus granulosus: characterization of the Spanish strains using in vitro vesicular development

Gordo

Bandera

1997

J. Helminthol.

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“…Yamashita et al (1962) also reported that the posterior bladders of E. multilocularis were involved in the development of cysts with a laminated layer, and furthermore suggested that the bladders arose from the attachment stalk of the protoscolex. In contrast, in the in vitro cultures of Gurri (1963), Benex (1968) and Brudnjak et al (1970) protoscoleces with posterior bladders did not produce cysts with a laminated layer.…”

Section: Introductionmentioning

confidence: 87%

“…A third morphological type -the posterior bladder -has also been described, in which a bladder-like vesicle is present at the posterior (abscolex) end (Yamashita, Ohbayashi, Sakamoto & Orihara, 1962;Smyth, 1962Smyth, ,1967Gurri, 1963;Pauluzzi, Sorice, Castagnari & Serra, 1965;Benex, 1968;Brudnjak, Cvetnic & Wikerhauser, 1970;Heath & Osborn, 1976). There is no consensus concerning the precise origin or fate of such bladders/ vesicles.…”

Section: Introductionmentioning

confidence: 99%

In vitro development of hydatid cysts from posterior bladders and ruptured brood capsules of equine Echinococcus granulosus

Rogan

Richards

1986

Parasitology

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A variety of morphological forms developed during monophasic culture of ruptured brood capsules of equine Echinococcus granulosus. Attached to some protoscoleces were small posterior bladders, with a surface ultrastructure of truncated microtriches, and these never developed a laminated layer. In contrast, small free vesicles of similar size and ultrastructure, but devoid of an attached protoscolex, produced a layer by 20-30 days. The surface ultrastructure of both these forms is similar to that of the protoscolex attachment stalk and the brood capsule lining, and posterior bladders and small free vesicles are considered to be derived from portions of the stalk +/- part of the brood capsule lining retained at protoscolex release. Also present were large vesicles interpreted from their size and surface anatomy as ruptured, everted brood capsules. When devoid of attached protosoleces these formed cysts each with a laminated layer, whereas similar vesicles with several attached protoscoleces did not develop a layer, or only very occasionally after 70 or more days. In contrast, a few large vesicles with a single attached protoscolex produced a laminated layer. The possibility that protoscolex attachment to stalk remnants or brood capsules inhibits the cystic development of these structures is discussed.

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“…There are many reports of the culture in vitro of Echinococcus granulosus metacestodes from protoscoleces derived from fertile hydatid cysts (Smyth, 1962;Benex, 1968;Brudnjak, Cvetnic & Wikerhauser, 1970;Heath & Osborn, 1976). Such material is relatively safe for humans to handle, because secondary echinococcosis should only result if protoscoleces were accidentally implanted parenterally.…”

Section: Introductionmentioning

confidence: 99%

Echinococcus granulosus: developmentin vitrofrom oncosphere to immature hydatid cyst

Heath

Lawrence

1976

Parasitology

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Echinococcus granulosuscysts were culturedin vitrofrom the oncosphere, and cyst morphogenesis is described. Cysts grew to a mean diameter of 16 mm in 120 days, with a maximum of 20 mm. An amorphous layer was apparent around the larvae during early post-oncospheral re-organization. Organisms became vesicular at 6 days and laminations appeared in the amorphous layer at 10 days. Confluence of proliferating cells to form the syncytium of the germinal membrane was observed.

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Considérations expérimentales nouvelles sur l’évolution in vitro en milieu liquide des larves d’Echinococcus granulosus

Cited by 14 publications

References 2 publications

Echinococcus granulosus: characterization of the Spanish strains using in vitro vesicular development

Echinococcus granulosus: characterization of the Spanish strains using in vitro vesicular development

In vitro development of hydatid cysts from posterior bladders and ruptured brood capsules of equine Echinococcus granulosus

Echinococcus granulosus: developmentin vitrofrom oncosphere to immature hydatid cyst

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