Constancy of Cross‐Reactivity Patterns in Sera of Individual Mice during the Anti‐H‐2 Response

Abstract

H‐2 alloimmunization evokes antibodies of differing cross‐reactivity patterns among individual mice of one inbred strain. We investigated whether this is due to unknown variations at the time of bleeding or whether it remains constant for an individual mouse during long‐term immunization. At various times during immunization, serum was collected from individual mice and it was tested on a panel of mouse and human lymphocytes. Sera from three donor‐recipient combinations were examined. In some sera, a broadening of the cross‐reactivity pattern occurred after prolonged immunization, but no major change in specificity was observed. We conclude that the differences in cross‐reactivity pattern among sera from individual mice remain constant during the immunization period and do not reflect variations in the condition of the animals at the time of bleeding.

Allo‐immune anti‐Iak sera of individual mice detect HLA—DR‐associated polymorphism on human B cells

“…Individual mice of one inbred strain differ in their immune response to complex MHS immunogens (Ivanyi et al 1978). The precise mechanism of this phenomenon is unclear, but is probably due to the clonal diversification of precursor cells and clonal dominance that occurs before immunization (Ivanyi & de Greeve 1978, Breuning et al 1980, Sherman 1980, Wettstein 1981, Cerny et al 1982 which are homologous to murine I-A antigens (Goyert et al 1982); (e) preliminary results refer to a "DR7 disease" which occurs only in some D R 7 homozygous individuals as a benign persistent polyclonal B cell proliferation (Gordon et al 1982). It is possible that the above-mentioned experimental results reflect an Ir gene dcfect on DR7 haplotypes that evokes the initiation of compensatory mechanisms or results in ineffective chronic proliferation of the B-cell pool.…”