CONSTANS and the CCAAT Box Binding Complex Share a Functionally Important Domain and Interact to Regulate Flowering of<i>Arabidopsis</i>

Wenkel, Stephan; Turck, Franziska; Singer, Kamy; Gissot, Lionel; Gourrierec, José Le; Samach, Alon; Coupland, George

doi:10.1105/tpc.106.043299

Cited by 557 publications

(596 citation statements)

References 72 publications

(100 reference statements)

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“…6 Among other things, HAP factors are known to be involved in the regulation of flowering and to bind to CCAAT box motifs in the promoter region of their target genes. 18 Two such CCAAT boxes can be found in the AMI1 promoter, 266 and 462 bp upstream of the start codon, respectively. Our hypothesis is further supported by the expression pattern of the corresponding genes as can be taken from publicly available databases (www.genevestigator.com/gv/index.jsp).…”

Section: Ami1 Expression Is Presumably Suppressed By Lec1mentioning

confidence: 99%

Expression ofAMIDASE1(AMI1) is suppressed during the first two days after germination

Hoffmann

Lehmann²,

Neu³

et al. 2010

Plant Signaling & Behavior

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T he regulation of cellular auxin levels is a critical factor in determining plant growth and architecture, as indole-3-acetic acid (IAA) gradients along the plant axis and local IAA maxima are known to initiate numerous plant growth responses. The regulation of auxin homeostasis is mediated in part by transport, conjugation and deconjugation, as well as by de novo biosynthesis. However, the pathways of IAA biosynthesis are yet not entirely characterized at the molecular and biochemical level. It is suggested that several biosynthetic routes for the formation of IAA have evolved. One such pathway proceeds via the intermediate indole-3-acetamide (IAM), which is converted into IAA by the activity of specific IAM hydrolases, such as Arabidopsis AMIDASE1 (AMI1). In this article we present evidence to support the argument that AMI1-dependent IAA synthesis is likely not to be used during the first two days of seedling development.Auxins are versatile plant hormones that play diverse roles in regulating many aspects of plant growth and development. 1 To enable auxins to develop their activity, a tight spatiotemporal control of cellular indole-3-acetic acid (IAA) contents is absolutely necessary since it is well-documented that auxin action is dose dependent, and that high IAA levels can have inhibitory effects on plant growth. 2 To achieve this goal, plants have evolved a set of different mechanisms to control cellular hormone levels. On the one hand, plants possess several pathways

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Section: Ami1 Expression Is Presumably Suppressed By Lec1mentioning

confidence: 99%

Expression ofAMIDASE1(AMI1) is suppressed during the first two days after germination

Hoffmann

Lehmann²,

Neu³

et al. 2010

Plant Signaling & Behavior

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“…It has been suggested that photoperiod-dependent flowering involves nuclear factor Y (NF-Y) complexes, which are conserved combinatorial transcription factors in eukaryotes consisting of NF-YA, NF-YB and NF-YC subunits [28][29][30][31][32] . NF-Y and its binding element, the CCAAT box, are among the first set of trans-acting factors and cis-elements identified in eukaryotes 33 .…”

mentioning

confidence: 99%

Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis

et al. 2014

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Nuclear factor Y (NF-Y) is a conserved heterotrimeric transcription factor complex that binds to the CCAAT motifs within the promoter region of many genes. In plants, a large number of genes code for variants of each NF-YA, B or C subunit that can assemble in a combinatorial fashion. Here, we report the discovery of an Arabidopsis NF-Y complex that exerts epigenetic control over flowering time by integrating environmental and developmental signals. We show that NF-Y interacts with CONSTANS in the photoperiod pathway and DELLAs in the gibberellin pathway, to directly regulate the transcription of SOC1, a major floral pathway integrator. This NF-Y complex binds to a unique cis-element within the SOC1 promoter to modulate trimethylated H3K27 levels, partly through a H3K27 demethylase REF6. Our findings establish NF-Y complexes as critical mediators of epigenetic marks that regulate the response to environmental or intrinsic signals in plants.

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“…In addition, recent systematic studies in Arabidopsis have revealed that physical interaction between AtNF-YB and AtNF-YC is required for the formation of the entire heterotrimeric complex, prior to binding to the consensus CCAAT motif, as shown for mammalian cells Hackenberg et al 2012). The AtNF-YC3, AtNF-YC4, and AtNF-YC9 proteins are also known to interact with the AtNF-YB2, AtNF-YB3, and CO subunits (Kumimoto et al 2010), suggesting that the AtNF-YC subunits make heterotrimeric complexes with AtNF-YB2 or AtNF-YB3 and CO to provide unique platforms for the finetuning of flowering under various environmental conditions (Wenkel et al 2006;Kumimoto et al 2010). Contrary to the canonical NF-Y complexes, CO/AtNF-YB/AtNF-YC trimeric complexes are speculated not to bind the CCAAT motif in the FT promoter, because the CO protein does not have the essential histidine residues required for NF-Y complex binding 6 (Xing et al 1993;Siefers et al 2009;Kumimoto et al 2010).…”

Section: Introductionmentioning

confidence: 92%

“…CO protein is known to interact with AtNF-YC3, 4 and 9 proteins via the CCT domain (Wenkel et al 2006;Kumimoto et al 2010). In wheat, VRN2 [acting as a flowering repressor (Yan et al 2004)] and CO2 [acting as a flowering promoter (Nemoto et al 2003)] also physically interact with the same subset of eight TaNF-Y proteins .…”

Section: Possible Interactions Between Osnf-yb or Osnf-yc Proteins Amentioning

confidence: 99%

“…Individual NF-Y subunits identified in plant species are also known to play an essential role in the regulation of developmental processes (Meinke et al 1994;Li et al 2008;Yamamoto et al 2009;Petroni et al 2012;Laloum et al 2013;Mu et al 2013). NF-YB and NF-YC subunits are found as CONSTANS (CO)-interacting proteins in tomato and Arabidopsis thaliana (BenNaim et al 2006;Wenkel et al 2006). The AtNF-YB2 and AtNF-YB3 genes are known to promote flowering through the activation of FLOWERING LOCUS T (FT) under long-day (LD) conditions in Arabidopsis (Cai et al 2007;Chen et al 2007;Kumimoto et al 2008).…”

Section: Introductionmentioning

confidence: 99%

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OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

Kim

Park

Jang

et al. 2015

Planta

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Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11).Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

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CONSTANS and the CCAAT Box Binding Complex Share a Functionally Important Domain and Interact to Regulate Flowering ofArabidopsis

Cited by 557 publications

References 72 publications

Expression ofAMIDASE1(AMI1) is suppressed during the first two days after germination

Expression ofAMIDASE1(AMI1) is suppressed during the first two days after germination

Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis

OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

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