Constitutive and regulated expression and secretion of interferon-γ-inducible protein 10 (IP-10/CXCL10) in human adipocytes

Herder, Christian; Hauner, Hans; Kempf, Kerstin; Kolb, Hubert; Skurk, Thomas

doi:10.1038/sj.ijo.0803432

Cited by 50 publications

(31 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…27 Various cell types, including macrophages, endothelial cells and fibroblasts, secrete IP-10 but also human adipocytes. 28,29 The results of our study, showing significantly higher IP-10 concentrations in the inferior mesenteric vein vs the subclavian vein, support the findings that VAT is an active site of IP-10 secretion.…”

Section: Discussionsupporting

confidence: 79%

Adipose tissue quantity and composition contribute to adipokine concentrations in the subclavian vein and the inferior mesenteric vein

et al. 2011

View full text Add to dashboard Cite

BACKGROUND: Adipose tissue dysfunction is associated with inflammation, type 2 diabetes mellitus and vascular diseases. Visceral adipose tissue (VAT)-derived adipokines, which are released in the portal circulation may influence liver metabolism. OBJECTIVES: (1) To estimate the contribution of VAT and subcutaneous adipose tissue (SAT) on adipokine levels by measuring differences in adipokine concentrations between the portal draining inferior mesenteric vein and the subclavian vein.(2) To determine the relation of both VAT and SAT quantity and composition to mesenteric and systemic concentrations of adipokines. DESIGN: Cross-sectional cohort study. SUBJECTS: A total of 32 patients undergoing abdominal aortic surgery. MEASUREMENTS: A panel of 18 adipokines was measured in perioperatively obtained blood samples from the subclavian vein and the inferior mesenteric vein. Adipocyte size, macrophage infiltration and capillary density were measured in subcutaneous and mesenteric adipose tissue biopsies; SAT and VAT areas were measured on computed tomography images. RESULTS: Serum interferon-g-inducible protein 10 (IP-10) and hepatocyte growth factor (HGF) concentrations were significantly higher in the inferior mesenteric vein vs the subclavian vein. SAT area (b --18; 95% confidence interval (CI) À35 to À2), subcutaneous adipocyte size (b --488; 95% CI À938 to À38) and SAT macrophages quantity (b --1439; 95% CI À2387 to À491) were negatively associated with adiponectin levels in the systemic circulation. SAT area was related to systemic concentrations of leptin. Mesenteric adiponectin concentrations were related to VAT area (b --20; 95% CI À35 to À5) and visceral adipocyte size (b À1076; 95% CI À1624 to À527). VAT area, adipocyte size and capillary density were related to systemic adiponectin concentrations. CONCLUSION: SAT and VAT quantities as well as morphologic characteristics of both adipose tissue depots are related to systemic and mesenteric adipokine concentrations. There were no differences in adipokine concentrations between the mesenteric and subclavian vein, except for higher IP-10 and HGF concentrations in the inferior mesenteric vein, indicating a possible contribution of VAT to IP-10 and HGF levels.

show abstract

Section: Discussionsupporting

confidence: 79%

Adipose tissue quantity and composition contribute to adipokine concentrations in the subclavian vein and the inferior mesenteric vein

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In preadipocytes and adipocytes, several groups have shown the expression of T-cell specific chemokines like RANTES, MCP-1, or IP-10. 4,17,18 We have also been able to detect RANTES in human adipose tissue, but additionally demonstrate the expression of SDF-1␣, another chemotactic protein for lymphocytes. 19 Immunohistochemical data suggest that SDF-1 within the adipose tissue stems from both endothelial cells as well as from preadipocytes/adipocytes.…”

Section: Kintscher Et Al T-lymphocytes and Insulin Resistancementioning

confidence: 86%

T-lymphocyte Infiltration in Visceral Adipose Tissue

Kintscher

Hartge²,

Heß³

et al. 2008

ATVB

611

321

View full text Add to dashboard Cite

Background— Adipose tissue inflammation may play a critical role in the pathogenesis of insulin resistance (IR). The present study examined the role of lymphocytes in adipose tissue inflammation and IR. Methods and Results— In a mouse model of obesity-mediated IR, high-fat diet (HFD) induced IR already after 5 weeks, which was associated with a marked T-lymphocyte infiltration in visceral adipose tissue. In contrast, recruitment of macrophages was delayed with an increase of MAC3-positive staining and F4/80 mRNA expression after 10 weeks of HFD, suggesting a dissociation of macrophage invasion into adipose tissue and IR initiation. In patients with type 2 diabetes, lymphocyte content in adipose tissue biopsies significantly correlated with waist circumference, a marker of IR. Immunohistochemical staining of human adipose tissue revealed the presence of mainly CD4-positive lymphocytes as well as macrophage infiltration. Most macrophages were HLA-DR–positive, reflecting activation through IFNγ, a cytokine released from CD4-positive lymphocytes. Conclusions— Proinflammatory T-lymphocytes are present in visceral adipose tissue and may contribute to local inflammatory cell activation before the appearance of macrophages, suggesting that these cells could play an important role in the initiation and perpetuation of adipose tissue inflammation as well as the development of IR.

show abstract

“…One of these molecules is the chemokine IP10, which is induced by IFNg-producing Th1 cells in many cell types (39)(40)(41). Serum IP10 increased in response to vitamin A supplementation and was positively associated with liver vitamin A stores.…”

Section: Discussionmentioning

confidence: 99%

Markers of Innate Immune Function Are Associated with Vitamin A Stores in Men

Ahmad

Haskell

Raqib

et al. 2009

The Journal of Nutrition

View full text Add to dashboard Cite

Recommendations for vitamin A intake and liver stores are based on maintaining normal vision. We propose that higher levels may be required to maintain normal innate immune function. To test this hypothesis, we conducted an 8-wk residential study among 36 healthy Bangladeshi men with low vitamin A stores. Subjects were randomized to receive vitamin A (240 mg in 4 doses) or placebo during study wk 2 and 3. They received 2 vaccines during wk 5 and vitamin A stores were estimated by isotopic dilution at wk 8. The serum concentration of the chemokine interferon-g-induced protein 10, a component of T-helper 1 (Th1) response, increased significantly after supplementation and was positively and significantly associated with vitamin A stores. Blood concentrations of natural killer (NK) and NK T-cells, which have anticancer and antiviral activity, were positively associated with stores (P , 0.05), as was monocyte oxidative burst (P , 0.05), a marker of bacterial killing ability. However, serum interleukin (IL)-6 and IL-17, cytokines that regulate the antibacterial Th17 response, were significantly and negatively associated with stores, as was production of the regulatory cytokine IL-10 by whole-blood cultures stimulated with bacterial lipopolysaccharide. In summary, vitamin A stores were positively associated with several measures of innate immune activity across a broad range of stores, suggesting that vitamin A enhances protection against diverse pathogens even at concentrations above those needed to maintain normal vision. The negative association of stores with serum IL-6 and IL-17 suggests that not all protective responses are similarly enhanced by vitamin A.

show abstract

Constitutive and regulated expression and secretion of interferon-γ-inducible protein 10 (IP-10/CXCL10) in human adipocytes

Cited by 50 publications

References 50 publications

Adipose tissue quantity and composition contribute to adipokine concentrations in the subclavian vein and the inferior mesenteric vein

Adipose tissue quantity and composition contribute to adipokine concentrations in the subclavian vein and the inferior mesenteric vein

T-lymphocyte Infiltration in Visceral Adipose Tissue

Markers of Innate Immune Function Are Associated with Vitamin A Stores in Men

Contact Info

Product

Resources

About