Constitutive chitosanase from Bacillus thuringiensis B-387 and its potential for preparation of antimicrobial chitooligomers

Актуганов, Г. Э.; Сафина, В. Р.; Галимзянова, Н. Ф.; Gilvanova, E.A.; Kuzmina, L. Yu.; Melentiev, A.I.; Baymiev, Andrei H.; Лопатин, С. А.

doi:10.1007/s11274-022-03359-5

Cited by 8 publications

(5 citation statements)

References 55 publications

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“…Meanwhile, the pH stability was determined by incubating the enzyme solutions with the pH buffers, including acetate-sodium buffer (pH 3.0–6.0), phosphate buffer (pH 6.0–8.0), Tris-HCl buffer (pH 8.0–9.0), and glycine–NaOH buffer (pH 9.0–10.0) at 37 °C for 1 h. And the optimal temperature of the enzyme was measured under reaction temperatures ranging from 30–70 °C. The thermal stability was determined by incubating the purified OUC-CsnPa at 30–70 °C for 1 h . The highest enzyme activity was defined as 100%.…”

Section: Methodsmentioning

confidence: 99%

Biochemical Characterization of a GH46 Chitosanase Provides Insights into the Novel Digestion Specificity

Zhao

Jia

et al. 2023

J. Agric. Food Chem.

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Endo-chitosanases (EC 3.2.1.132) are generally considered to selectively release functional chito-oligosaccharides (COSs) with degrees of polymerization (DPs) ≥ 2. Although numerous endo-chitosanases have been characterized, the digestion specificity of endo-chitosanases needs to be further explored. In this study, a GH46 endo-chitosanase OUC-CsnPa was cloned, expressed, and characterized from Paenibacillus sp. 1–18. The digestion pattern analysis indicated that OUC-CsnPa could produce monosaccharides from chitotetraose [(GlcN)4], the smallest recognized substrate, in a random endo-acting manner. Especially, the enzyme specificities during chitosan digestion including the regulation of product abundance through a transglycosylation reaction were also evaluated. It was hypothesized that an insertion region in OUC-CsnPa may form a strong force to be involved in stabilizing (GlcN)4 at its negative subsite for efficient hydrolysis. This is the first comprehensive report to reveal the digestion specificity and subsite specificity of monosaccharide production by endo-chitosanases. Overall, OUC-CsnPa described here highlights the previously unknown digestion properties of the endo-acting chitosanases and provides a unique example of possible structure–function relationships.

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Section: Methodsmentioning

confidence: 99%

Biochemical Characterization of a GH46 Chitosanase Provides Insights into the Novel Digestion Specificity

Zhao

Jia

et al. 2023

J. Agric. Food Chem.

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“…It is likely that the positive charges of COSs interact with negative charges on the bacterial cell surfaces, leading to inhibition of cell growth. In contrast, the shorter COSs (DA = 0.15; DP = 2, 3, and 4) and low molecular weight chitosan produced by a chitosanase from Bacillus thuringiensis B-387 from chitosan polysaccharide (DA, 0.15), of which the average MWs were in a range of 14–46 kDa, exhibited greater antibacterial activities toward E. coli in vitro (MIC, 70 mg·mL −1 ) in comparison with the initial polysaccharides (MIC, 120 mg·mL −1 ) [ 22 ]. Ganan et al analyzed the antifungal activities of soluble and well-defined COSs.…”

Section: Biological Activities Of Cossmentioning

confidence: 99%

“…In contrast, the shorter COSs (DA = 0. 2, 3, and 4) and low molecular weight chitosan produced by a chitosanase from thuringiensis B-387 from chitosan polysaccharide (DA, 0.15), of which the avera were in a range of 14-46 kDa, exhibited greater antibacterial activities toward vitro (MIC, 70 mg•mL −1 ) in comparison with the initial polysaccharides (M mg•mL −1 ) [22]. Ganan…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities

Thomas,

Fukamizo,

Suginta

2023

Molecules

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Chitooligosaccharides (COSs) are b-1,4-linked homo-oligosaccharides of N-acetylglucosamine (GlcNAc) or glucosamine (GlcN), and also include hetero-oligosaccharides composed of GlcNAc and GlcN. These sugars are of practical importance because of their various biological activities, such as antimicrobial, anti-inflammatory, antioxidant and antitumor activities, as well as triggering the innate immunity in plants. The reported data on bioactivities of COSs used to contain some uncertainties or contradictions, because the experiments were conducted with poorly characterized COS mixtures. Recently, COSs have been satisfactorily characterized with respect to their structures, especially the degree of polymerization (DP) and degree of N-acetylation (DA); thus, the structure–bioactivity relationship of COSs has become more unambiguous. To date, various green-chemical strategies involving enzymatic synthesis of COSs with designed sequences and desired biological activities have been developed. The enzymatic strategies could involve transglycosylation or glycosynthase reactions using reducing end-activated sugars as the donor substrates and chitinase/chitosanase and their mutants as the biocatalysts. Site-specific chitin deacetylases were also proposed to be applicable for this purpose. Furthermore, to improve the yields of the COS products, metabolic engineering techniques could be applied. The above-mentioned approaches will provide the opportunity to produce tailor-made COSs, leading to the enhanced utilization of chitin biomass.

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“…It was shown that oligosaccharides of chitin and chitosan produced with the participation of extracellular chitinases and chitosanases of Bt subsp. dendrolimus B-387 possess high bactericidal and fungicidal activity [122]. Similarly, endophytic B. cereus XB177R, which show high chitinase activity effectively protected eggplant plants from bacterial wilt caused by R. solanacearum [69].…”

Section: Production Of Fungicidal and Bactericidal Compounds And Trig...mentioning

confidence: 99%

Plant-Associated Bacillus thuringiensis and Bacillus cereus: Inside Agents for Biocontrol and Genetic Recombination in Phytomicrobiome

Sorokan,

Gabdrakhmanova,

Kuramshina

et al. 2023

Plants

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Bacillus thuringiensis Berliner (Bt) and B. cereus sensu stricto Frankland and Frankland are closely related species of aerobic, spore-forming bacteria included in the B. cereus sensu lato group. This group is one of the most studied, but it remains also the most mysterious species of bacteria. Despite more than a century of research on the features of these ubiquitous bacteria, there are a lot of questionable issues related to their taxonomy, resistance to external influences, endophytic existence, their place in multidimensional relationships in the ecosystem, and many others. The review summarizes current data on the mutualistic relationships of Bt and B. cereus bacteria with plants, the structure of the phytomicrobiomes including Bt and B. cereus, and the abilities of plant-associated and endophytic strains to improve plant resistance to various environmental factors and its productivity. Key findings on the possibility of the use of Cry gene promoter for transcription of the target dsRNA and simultaneous release of pore-forming proteins and provocation of RNA-interference in pest organisms allow us to consider this group of microorganisms as unique tools of genetic engineering and biological control. This will open the prospects for the development and direct change of plant microbiomes, and possibly serve as the basis for the regulation of the entire agroecosystem.

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Constitutive chitosanase from Bacillus thuringiensis B-387 and its potential for preparation of antimicrobial chitooligomers

Abstract: RAS: U mskij institut biologii RAN Nailya Galimzianova Ufa Scienti c Center of the Russian Academy of Sciences: FGBNU U mskij federal'nyj issledovatel'skij centr Rossijskoj akademii nauk

Cited by 8 publications

References 55 publications

Biochemical Characterization of a GH46 Chitosanase Provides Insights into the Novel Digestion Specificity

Biochemical Characterization of a GH46 Chitosanase Provides Insights into the Novel Digestion Specificity

Green-Chemical Strategies for Production of Tailor-Made Chitooligosaccharides with Enhanced Biological Activities

Plant-Associated Bacillus thuringiensis and Bacillus cereus: Inside Agents for Biocontrol and Genetic Recombination in Phytomicrobiome

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