The effect of different interpolation methods on imaging quality of swept-source optical coherence tomography (SSOCT) is analyzed. A 1310nm SS-OCT system is built for imaging. Time-domain interpolation method based on spectral phase is used for resampling. The interpolation methods include piecewise linear interpolation, piecewise quadratic parabola interpolation, piecewise cubic Hermite interpolation and piecewise cubic spline interpolation. The image of the plane mirror, human skin and eyes are compared by the above four interpolation methods. Experimental results demonstrate that the piecewise cubic spline interpolation has the best image quality, which is the smoothest and has the lowest noise.