Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes

Gǒng, Wànkuí; Liu, Wei; Lu, Yingying; Bian, Yinbing; Zhou, Yan; Kwan, Hoi Shan; Cheung, Man Kit; Xiao, Yang

doi:10.1016/j.funbio.2014.01.001

Cited by 30 publications

(25 citation statements)

References 55 publications

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“…Thus, we determined 13 such agronomic traits on our tested strains. Mycelium growth rates on both MYG (2% malt extract; 2% glucose; 2% agar; 0.1% peptone; 0.1% yeast extract) medium and mixed sawdust (SD) (DGR-myg and DGR-sd) were measured for all 60 strains by using methods described previously3334.…”

Section: Methodsmentioning

confidence: 99%

Population genomic analysis uncovers environmental stress-driven selection and adaptation of Lentinula edodes population in China

Xiao

Cheng

Liu

et al. 2016

Sci Rep

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The elucidation of genome-wide variations could help reveal aspects of divergence, domestication, and adaptation of edible mushrooms. Here, we resequenced the whole genomes of 39 wild and 21 cultivated strains of Chinese Lentinula edodes, the shiitake mushroom. We identified three distinct genetic groups in the Chinese L. edodes population with robust differentiation. Results of phylogenetic and population structure analyses suggest that the cultivated strains and most of the wild trains of L. edodes in China possess different gene pools and two outlier strains show signatures of hybridization between groups. Eighty-four candidate genes contributing to population divergence were detected in outlier analysis, 18 of which are involved in response to environmental stresses. Gene enrichment analysis of group-specific single nucleotide polymorphisms showed that the cultivated strains were genetically diversified in biological processes related to stress response. As the formation of fruiting bodies is a stress-response process, we postulate that environment factors, such as temperature, drove the population divergence of L. edodes in China by natural or artificial selection. We also found phenotypic variations between groups and identified some wild strains that have potential to diversify the genetic pool for improving agricultural traits of L. edodes cultivars in China.

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Section: Methodsmentioning

confidence: 99%

Population genomic analysis uncovers environmental stress-driven selection and adaptation of Lentinula edodes population in China

Xiao

Cheng

Liu

et al. 2016

Sci Rep

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“…According to our previous study [6], the experimental groups were subjected to 39 °C and 40 °C heat stress (HS) for 24h after 6 days of normal growth at 25 °C. Meanwhile, five strains screened in the thermotolerance assay were inoculated into a test tube containing the mixed sawdust medium containing 79% hardwood sawdust, 20% wheat bran, 1% gypsum, and 60% humidity [34]. After 6 days of normal growth at 25 °C, the mycelial elongation position in each test-tube was marked as the starting point, and the test-tubes were subjected to 39 °C, 40 °C and 41 °C heat stress for 24 hours.…”

Section: Mushroom Strains Growth Conditions and Thermotolerance Assaymentioning

confidence: 99%

Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

Wang

Luo

et al. 2018

Cell Physiol Biochem

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Background/Aims: Heat stress could cause huge losses for Lentinula edodes in China and other Asian cultivation areas. Yet our understanding of mechanism how to defend to heat stress is incomplete. Methods: Using heat-tolerant and heat-sensitive strains of L. edodes, we reported a combined proteome and transcriptome analysis of L. edodes response to 40 °C heat stress for 24 h. Meanwhile, the effect of LeDnaJ on the thermotolerance and IAA (indoleacetic acid) biosynthesis in L. edodes was analyzed via the over-expression method. Results: The proteome results revealed that HSPs (heat shock proteins) such as Hsp40 (DnaJ), Hsp70, Hsp90 and key enzymes involved in tryptophan and IAA metabolism process LeTrpE, LeTrpD, LeTam-1, LeYUCCA were more highly expressed in S606 than in YS3357, demonstrating that HSPs and tryptophan as well as IAA metabolism pathway should play an important role in thermotolerance. Over-expression of LeDnaJ gene in S606 strains showed better tolerance to heat stress. It was also documented that intracellular IAA accumulation of S606 (8-fold up) was more than YS3357 (2-fold up), and exogenous IAA enhanced L. edodes tolerance to heat stress. Conclusion: Our data support the interest of LeTrpE, LeDnaJ, tryptophan and IAA could play a pivotal role in enhancing organism thermotolerance.

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“…For all the 127 SSIs, mgrs were determined on the malt extract agar medium, which were calculated as the radial extension of each mycelial colony per day (Gong et al, 2014). All the SSIs were incubated at 25 • C in the dark with three repetitions.…”

Section: Quantitative Trait Locus Mapping Of Monokaryon Growthmentioning

confidence: 99%

A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth

et al. 2020

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Hericium erinaceus has attracted tremendous interest owing to its compelling healthpromoting properties. However, breeding of elite cultivars of H. erinaceus is hindered by the lack of a genetic and molecular toolbox. Here, we performed resequencing analysis of 127 F 1 single-spore isolates and constructed the first high-resolution genetic map of H. erinaceus. With the use of recombination bins as markers, an ultradense genetic map consisting of 1,174 bins (including 37,082 single-nucleotide polymorphisms) was generated. This newly developed genetic map covered 1,096.5 cM, with an average bin spacing of 0.95 cM. High collinearity between genetic map and H. erinaceus genome assembly was revealed by aligning scaffolds to this genetic map using bin markers as anchors. The application of this newly developed genetic map in quantitative trait locus (QTL) mapping was also elucidated, and four QTLs for monokaryon growth were recovered. One QTL, mgr1, which contributes 12.1% of growth variations, was located near the mating type A (MAT-A) loci. Overall, this newly constructed high-resolution genetic map (or bin map) could be used as reference in future genetic, genomic, and breeding studies on H. erinaceus.

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Constructing a new integrated genetic linkage map and mapping quantitative trait loci for vegetative mycelium growth rate in Lentinula edodes

Cited by 30 publications

References 55 publications

Population genomic analysis uncovers environmental stress-driven selection and adaptation of Lentinula edodes population in China

Population genomic analysis uncovers environmental stress-driven selection and adaptation of Lentinula edodes population in China

Proteome and Transcriptome Reveal Involvement of Heat Shock Proteins and Indoleacetic Acid Metabolism Process in Lentinula Edodes Thermotolerance

A Resequencing-Based Ultradense Genetic Map of Hericium erinaceus for Anchoring Genome Sequences and Identifying Genetic Loci Associated With Monokaryon Growth

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