Construction and application of a new class of sequential oligopeptide carriers (SOCn) for multiple anchoring of antigenic peptides-application to the acetylcholine receptor (AChR) main immunogenic region

Tsikaris, Vassilios; Sakarellos, Constantinos; Sakarellos‐Daitsiotis, Maria; Orlewski, Piotr; Marraud, Michel; Cung, Manh Thông; Vatzaki, E.; Tzartos, Socrates J.

doi:10.1016/0141-8130(96)01128-2

Cited by 26 publications

(25 citation statements)

References 18 publications

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“…These findings indicate a clear advantage in using [Ala 76 ]MIR-SOC n -I rather than MIR peptides as potent antigens in diagnostic assays or in elaborating affinity chromatography resins for antibody depletion (plasmapheresis) in MG patient sera (Fig. 10) [95,105,108].…”

Section: Myasthenia Gravis (Mg) and The Main Immunogenic Region (Mir)mentioning

confidence: 92%

“…The structural characterization of SOC n -I was carried out by 1 H-NMR experiments and molecular modeling [108]. Intense NOE connectivities, appearing between successive amide protons, are compatible either with a helical conformation or with a random structure.…”

Section: Conformational Analysis Of the Soc N -I Carriermentioning

confidence: 99%

“…In order to refine the SOC n -I structure, the preceding NMR data were introduced in MD calculations [108]. Considering the main conformational angles of the time -averaged structure and the average inter-proton distances, estimated from NOE measurements, a time-averaged structure stabilized by a network of hydrogen bonds was obtained ( Table 2).…”

Section: Ac-k-aib-g-k-aib-g-k-aib-g-k-aib-g-pam Resinmentioning

confidence: 99%

“…Stereoviews of the minimized average structure of the SOC 4 peptide carrier from the last 20 ps of in vacuo restrained MD simulation compared to the ribbon representing the SOC 4 backbone in a canonical 3 10 -helix, adapted by Tsikaris et al[108].…”

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confidence: 99%

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Artificial Carriers: A Strategy for Constructing Antigenic/Immunogenic Conjugates

Sakarellos‐Daitsiotis¹,

Krikorian²,

Panou‐Pomonis³

et al. 2006

CTMC

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The rational design of artificial carriers for anchoring multiple copies of B and/or T cell epitopes, built-in vaccine adjuvants and "promiscuous" T cell epitopes for the construction of conjugates as antigenic substrates or potent immunogens has been the stimulus of intensive efforts nowadays. The unambiguous composition, the reliability and the versatility of the production of reconstituted antigens or immunogens has found a great number of biochemical applications in developing immunoassays of high sensitivity, specificity and reproducibility and in generating site-specific antibodies for usage as human vaccine candidates. In this review are summarized different types of artificial carriers currently used as dendrimers bearing branching segments, multimeric core matrices and templates with built-in folding devices. Emphasis is given to the construction and application of a helicoid-type Sequential Oligopeptide Carrier (SOCn) developed in our laboratory. The beneficial structural elements of SOCn induce a favorable arrangement of the conjugated peptides, which also retain their initial "active" conformation, so that potent antigens and immunogens are generated.

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Section: Myasthenia Gravis (Mg) and The Main Immunogenic Region (Mir)mentioning

confidence: 92%

Section: Conformational Analysis Of the Soc N -I Carriermentioning

confidence: 99%

Section: Ac-k-aib-g-k-aib-g-k-aib-g-k-aib-g-pam Resinmentioning

confidence: 99%

mentioning

confidence: 99%

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Artificial Carriers: A Strategy for Constructing Antigenic/Immunogenic Conjugates

Sakarellos‐Daitsiotis¹,

Krikorian²,

Panou‐Pomonis³

et al. 2006

CTMC

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“…SOCs are linear molecules with a Lys-Aib-Gly tripeptide sequential motif. By varying the number of tripeptide units, carriers of general formula SOC n , (Lys-Aib-Gly) n , where n=2-7 have been prepared [74]. The molecules of carriers adopt a predetermined secondary structure which is given by the presence of Aib, an unnatural amino acid with a known propensity to induce ordered helicoid backbone [75].…”

Section: Sequential Oligopeptide Carriers In Multiple Antigen Peptidementioning

confidence: 99%

Peptide and glycopeptide dendrimers. Part II

Vepřek

Ježek

1999

J. Peptide Sci.

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Recent progress in peptide and glycopeptide chemistry make the preparation of peptide and glycopeptide dendrimers of acceptable purity, with designed structural and immunochemical properties reliable. New methodologies using unprotected peptide building blocks have been developed to further increase the possibilities of their design and improve their preparation and separation. The sophisticated design of peptide and glycopeptide dendrimers has led to their use as antigens and immunogens, for serodiagnosis and other biochemical uses including drug delivery. Dendrimers bearing peptide with predetermined secondary structures are useful tools in protein de novo design. This article covers synthesis and applications of multiple antigen peptides (MAPs), multiple antigen glycopeptides (MAGs), multiple antigen peptides based on sequential oligopeptide carriers (MAP-SOCs), glycodendrimers and template-assembled synthetic proteins (TASPs). In part II the preparation of MAPs, and the utility of glycodendrimers and TASPs are discussed.

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The position of the LysN?H2-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)n (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope

Alexopoulos¹,

Tsikaris²,

Rizou³

et al. 2000

Biopolymers

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Construction and application of a new class of sequential oligopeptide carriers (SOCn) for multiple anchoring of antigenic peptides-application to the acetylcholine receptor (AChR) main immunogenic region

Cited by 26 publications

References 18 publications

Artificial Carriers: A Strategy for Constructing Antigenic/Immunogenic Conjugates

Artificial Carriers: A Strategy for Constructing Antigenic/Immunogenic Conjugates

Peptide and glycopeptide dendrimers. Part II

The position of the LysN?H2-grafted antigens along the sequential oligopeptide carrier, Ac-(Aib-Lys-Aib-Gly)n (SOCn-II), influences the antibody recognition: Application to the Sm main autoimmune epitope

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