Construction and Catalytic Study of Affinity Peptide Orientation and Light Crosslinking Immobilized Sucrose Isomerase

Shi, Nian; Zheng, Mingqiang; Wu, Xinming; Chen, Ning; Jiang, Luying; Chang, Baogen; Lu, Fuping; Liu, Fufeng

doi:10.1021/acs.jafc.3c02644

Cited by 2 publications

(2 citation statements)

References 43 publications

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“…This method utilizes the photo-cross-linking reaction to covalently fix the enzyme molecules onto the epoxy resin, making it less prone to detachment and improving its reusability. After 11 cycles, the relative enzyme activity of the method can be maintained by more than 50% [69].…”

Section: Directional Immobilizationmentioning

confidence: 99%

A Critical Review on Immobilized Sucrose Isomerase and Cells for Producing Isomaltulose

Jing,

Hou,

et al. 2024

Foods

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Isomaltulose is a novel sweetener and is considered healthier than the common sugars, such as sucrose or glucose. It has been internationally recognized as a safe food product and holds vast potential in pharmaceutical and food industries. Sucrose isomerase is commonly used to produce isomaltulose from the substrate sucrose in vitro and in vivo. However, free cells/enzymes were often mixed with the product, making recycling difficult and leading to a significant increase in production costs. Immobilized cells/enzymes have the following advantages including easy separation from products, high stability, and reusability, which can significantly reduce production costs. They are more suitable than free ones for industrial production. Recently, immobilized cells/enzymes have been encapsulated using composite materials to enhance their mechanical strength and reusability and reduce leakage. This review summarizes the advancements made in immobilized cells/enzymes for isomaltulose production in terms of refining traditional approaches and innovating in materials and methods. Moreover, innovations in immobilized enzyme methods include cross-linked enzyme aggregates, nanoflowers, inclusion bodies, and directed affinity immobilization. Material innovations involve nanomaterials, graphene oxide, and so on. These innovations circumvent challenges like the utilization of toxic cross-linking agents and enzyme leakage encountered in traditional methods, thus contributing to enhanced enzyme stability.

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Section: Directional Immobilizationmentioning

confidence: 99%

A Critical Review on Immobilized Sucrose Isomerase and Cells for Producing Isomaltulose

Jing,

Hou,

et al. 2024

Foods

Self Cite

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“…Enzyme immobilization technology refers to the process of fixing enzymes within a defined spatial range using physical or chemical means to protect them from environmental factors and enhance their stability [4]. Common enzyme immobilization methods include adsorption [5][6][7][8][9], encapsulation [10][11][12][13][14], and cross-linking [15][16][17][18][19]. Apart from the impact of immobilization methods, the choice of immobilization carriers also significantly influences the catalytic performance of enzymes [20].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Strategies of Simultaneous Enzyme Immobilization Accompanied by Nanocarrier Synthesis

Hao,

Liu,

Chu

2024

Applied Sciences

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In recent years, with advancements in nanotechnology and materials science, new enzyme immobilization strategies based on nanomaterials have continuously emerged. These strategies have shown significant effects on enhancing enzyme catalytic performance and stability due to their high surface area, good chemical stability, and ease of enzyme binding, demonstrating tremendous potential for industrial applications. Those methods that can rapidly synthesize nanocarriers under mild conditions allow for the one-step synthesis of nanocarriers and enzyme complexes, thereby exhibiting advantages such as simplicity of process, minimal enzyme damage, short processing times, and environmental friendliness. This paper provides an overview of simultaneous enzyme immobilization strategies accompanied by nanocarrier synthesis, including organic–inorganic hybrid nano-flowers (HNFs), metal–organic frameworks (MOFs), and conductive polymers (CPs). It covers their preparation principles, post-immobilization performance, applications, and existing challenges.

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Construction and Catalytic Study of Affinity Peptide Orientation and Light Crosslinking Immobilized Sucrose Isomerase

Cited by 2 publications

References 43 publications

A Critical Review on Immobilized Sucrose Isomerase and Cells for Producing Isomaltulose

A Critical Review on Immobilized Sucrose Isomerase and Cells for Producing Isomaltulose

Recent Advances in the Strategies of Simultaneous Enzyme Immobilization Accompanied by Nanocarrier Synthesis

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