Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

Cenci, Alberto; Chantret, Nathalie; Kong, Xiuying; Gu, Yong; Anderson, Olin D.; Fahima, Tzion; Distelfeld, Assaf; Dubcovsky, Jorge

doi:10.1007/s00122-003-1331-z

Cited by 121 publications

(109 citation statements)

References 65 publications

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“…We first obtained complete genomic sequences of VRN-1 including approximately 500 bp of the 5¢ and 300 bp of the 3¢ regions from BAC clones using overlapping PCR fragments. We sequenced this gene from BACs 631P8 (VRN-H1, AY750995) from the barley variety Morex (Yu et al 2000), 1256C17 (VRN-A1, AY747598) and 1225D16 (VRN-B1, AY747602) from the tetraploid wheat variety Langdon (Cenci et al 2003), and 22J2 (VRN-D1, AY747605) from an EcoRI BAC library of T. tauschii (Xu et al 2002).…”

Section: Cloning and Sequencingmentioning

confidence: 99%

Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat

et al. 2005

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The broad adaptability of wheat and barley is in part attributable to their flexible growth habit, in that spring forms have recurrently evolved from the ancestral winter growth habit. In diploid wheat and barley growth habit is determined by allelic variation at the VRN-1 and/or VRN-2 loci, whereas in the polyploid wheat species it is determined primarily by allelic variation at VRN-1. Dominant Vrn-A1 alleles for spring growth habit are frequently associated with mutations in the promoter region in diploid wheat and in the A genome of common wheat. However, several dominant Vrn-A1, Vrn-B1, Vrn-D1 (common wheat) and Vrn-H1 (barley) alleles show no polymorphisms in the promoter region relative to their respective recessive alleles. In this study, we sequenced the complete VRN-1 gene from these accessions and found that all of them have large deletions within the first intron, which overlap in a 4-kb region. Furthermore, a 2.8-kb segment within the 4-kb region showed high sequence conservation among the different recessive alleles. PCR markers for these deletions showed that similar deletions were present in all the accessions with known Vrn-B1 and Vrn-D1 alleles, and in 51 hexaploid spring wheat accessions previously shown to have no polymorphisms in the VRN-A1 promoter region. Twenty-four tetraploid wheat accessions had a similar deletion in VRN-A1 intron 1. We hypothesize that the 2.8-kb conserved region includes regulatory elements important for the vernalization requirement. Epistatic interactions between VRN-H2 and the VRN-H1 allele with the intron 1 deletion suggest that the deleted region may include a recognition site for the flowering repression mediated by the product of the VRN-H2 gene of barley.

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Section: Cloning and Sequencingmentioning

confidence: 99%

Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat

et al. 2005

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“…PSY-A1 and PSY-B1 genome-speciWc primers (Table 1) were used to screen the tetraploid wheat BAC library from variety "Langdon" (Cenci et al 2003). BAC clones 313-J21 (PSY-A1) and 474-K06 (PSY-B1) were used to obtain the complete genomic sequences of the PSY-1 genes.…”

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confidence: 99%

Association between allelic variation at the Phytoene synthase 1 gene and yellow pigment content in the wheat grain

2008

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A better understanding of the genetic factors controlling grain yellow pigment content (GYPC) is important for both pasta (high GYPC) and bread wheat (low GYPC) quality improvement. Quantitative trait loci (QTL) for GYPC have been mapped repeatedly on the distal regions of chromosome arms 7AL and 7BL in wheat, and the Phytoene synthase 1 (PSY-1) gene located in this region has been proposed as a candidate gene. We show here that PSY-E1, the tall wheatgrass orthologue, is completely linked to diVerences in GYPC, and that selection for white endosperm mutants in recombinant lines carrying this gene resulted in the identiWcation of a mutation in a conserved amino acid of PSY-E1. These results, together with the association between GYPC and allelic diVerences in PSY-1 in hexaploid wheat, suggest that this gene plays an important role in the determination of GYPC. However, a second white endosperm mutant previously mapped to chromosome arm 7EL showed no mutations in PSY-E1 suggesting the existence of additional gene(s) aVecting GYPC in this chromosome region. This hypothesis was further supported by the mapping of QTL for GYPC on 7AL proximal to PSY-1 in a cross between pasta wheat varieties UC1113 and Kofa. Interestingly, the Kofa PSY-B1 allele showed unusually high levels of polymorphisms as a result of a conversion event involving the PSY-A1 allele. In summary, our results support the hypothesis that allelic diVerences in PSY-1 and at least one additional gene in the distal region of the long arm of homoeologous group 7L are associated with diVerences in GYPC.

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“…The precise mapping of the Gpc-6B1 gene reported in this study, and the recent completion of a 5 BAC library of RSL65 (Cenci et al 2003) represent two fundamental steps in the efforts of our laboratory towards the positional cloning of this important agronomic gene.…”

Section: Marker-assisted Selection (Mas)mentioning

confidence: 91%

Precise mapping of a locus affecting grain protein content in durum wheat

et al. 2003

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Grain protein content (GPC) is an important factor in pasta and breadmaking quality, and in human nutrition. It is also an important trait for wheat growers because premium prices are frequently paid for wheat with high GPC. A promising source for alleles to increase GPC was detected on chromosome 6B of Triticum turgidum var. dicoccoides accession FA-15-3 (DIC). Two previous quantitative trait locus (QTL) studies found that the positive effect of DIC-6B was associated to a single locus located between the centromere and the Nor-B2 locus on the short arm of chromosome 6B. Microsatellite markers Xgwm508 and Xgwm193 flanking the QTL region were used in this study to develop 20 new homozygous recombinant substitution lines (RSLs) with crossovers between these markers. These 20 RSLs, plus nine RSLs developed in previous studies were characterized with four new RFLP markers located within this chromosome segment. Grain protein content was determined in three field experiments organized as randomized complete block designs with ten replications each. The QTL peaks for protein content were located in the central region of a 2.7-cM interval between RFLP markers Xcdo365 and Xucw67 in the three experiments. Statistical analyses showed that almost all lines could be classified unequivocally within low-and high-protein groups, facilitating the mapping of this trait as a single Mendelian locus designated Gpc-6B1. The Gpc-6B1 locus was mapped 1.5-cM proximal to Xcdo365 and 1.2-cM distal to Xucw67. These new markers can be used to reduce the size of the DIC chromosome segment selected in markerassisted selection programs. Markers Nor-B2 and Xucw66 flanking the previous two markers can be used to select against the DIC segment and reduce the linkage drag during the transfer of Gpc-6B1 into commercial bread and pasta wheat varieties. The precise mapping of the high GPC gene, the high frequency of recombinants recovered in the targeted region, and the recent development of a tetraploid BAC library including the Gpc-6B1 DIC allele are the first steps towards the map-based cloning of this gene.

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Construction and characterization of a half million clone BAC library of durum wheat (Triticum turgidum ssp. durum)

Cited by 121 publications

References 65 publications

Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat

Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat

Association between allelic variation at the Phytoene synthase 1 gene and yellow pigment content in the wheat grain

Precise mapping of a locus affecting grain protein content in durum wheat

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