Construction and characterization of a cDNA library from shark regenerated hepatic tissue

Zhou, Feng; Wang, Ying; Guan, Yue; Xu, Ya; Gao, Xiangdong; Wu-tong, WU; Ye, Boping

doi:10.1016/j.fsi.2011.02.025

Cited by 9 publications

(2 citation statements)

References 52 publications

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“…The PCR products were detected with gel electrophoresis. The positive control was 18s rRNA [4]. All of the primers that were used for RT-PCR are listed in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark,Chiloscyllium plagiosum

Zhang

Nie

et al. 2013

BioMed Research International

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To understand the mechanisms of liver regeneration better to promote research examining liver diseases and marine biology, normal and regenerative liver tissues of Chiloscyllium plagiosum were harvested 0 h and 24 h after partial hepatectomy (PH) and used to isolate small RNAs for miRNA sequencing. In total, 91 known miRNAs and 166 putative candidate (PC) miRNAs were identified for the first time in Chiloscyllium plagiosum. Through target prediction and GO analysis, 46 of 91 known miRNAs were screened specially for cellular proliferation and growth. Differential expression levels of three miRNAs (xtr-miR-125b, fru-miR-204, and hsa-miR-142-3p_R-1) related to cellular proliferation and apoptosis were measured in normal and regenerating liver tissues at 0 h, 6 h, 12 h, and 24 h using real-time PCR. The expression of these miRNAs showed a rising trend in regenerative liver tissues at 6 h and 12 h but exhibited a downward trend compared to normal levels at 24 h. Differentially expressed genes were screened in normal and regenerating liver tissues at 24 h by DDRT-PCR, and ten sequences were identified. This study provided information regarding the function of genes related to liver regeneration, deepened the understanding of mechanisms of liver regeneration, and resulted in the addition of a significant number of novel miRNAs sequences to GenBank.

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“…The PCR products were detected with gel electrophoresis. The positive control was 18s rRNA [4]. All of the primers that were used for RT-PCR are listed in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark,Chiloscyllium plagiosum

Zhang

Nie

et al. 2013

BioMed Research International

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“…In contrast to other animals, C. plagiosum has a large liver, accounting for approximately 75% of the total weight of its internal organs. The liver is not only an important organ with detoxification function but also has a strong regeneration ability, which endows C. plagiosum with many special characteristics, such as strong immune regulation (Zhou et al, 2011;Wang et al, 2013;Zhang et al, 2013;Masstor et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Identification of circRNAs in the Liver of Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Zhang¹,

Qin²,

Gong³

et al. 2020

Front. Genet.

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Whitespotted bamboo shark (Chiloscyllium plagiosum), a member of the cartilaginous fish family, has an extremely large liver and demonstrates a strong regeneration ability and immune regulation. Circular RNAs (circRNAs) is an important class of non-coding RNAs. Increasing evidences suggest that circRNAs are a kind of potential regulators. Recently, researchers have isolated and identified different circRNAs from various species, while few reports were on the circRNAs of C. plagiosum. In this study, we have identified a total of 4,558 circRNAs in the liver of C. plagiosum. This finding suggests that circRNAs are not evenly distributed in the chromosomes and follow the GT-AG rule during cyclization. Alternative back-splicing might exist in shark circRNAs as shown by the authenticity identification of predicted circRNAs. The binding strength of circRNAs (<2,000 bp) and the detected miRNAs in shark liver were simultaneously analyzed to construct an mRNA–miRNA–circRNA network for the Glutathione S-transferase P1 gene, and the circRNA authenticity was simultaneously verified. Our data provide not only novel insights into the rich existence of circRNAs in marine animals, but also a basis for characterizing functions of identified circRNAs in the liver homeostasis of C. plagiosum.

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Construction and characterization of a full-length cDNA library and identification of genes involved in salinity stress in wild eggplant (Solanum torvum Swartz)

Chen

Wang

Gai

et al. 2012

Hortic. Environ. Biotechnol.

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Construction and characterization of a cDNA library from shark regenerated hepatic tissue

Cited by 9 publications

References 52 publications

Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark,Chiloscyllium plagiosum

Study of MicroRNAs Related to the Liver Regeneration of the Whitespotted Bamboo Shark,Chiloscyllium plagiosum

Identification of circRNAs in the Liver of Whitespotted Bamboo Shark (Chiloscyllium plagiosum)

Construction and characterization of a full-length cDNA library and identification of genes involved in salinity stress in wild eggplant (Solanum torvum Swartz)

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