Construction and Immunogenicity of Novel Chimeric Virus-Like Particles Bearing Antigens of Infectious Bronchitis Virus and Newcastle Disease Virus

Abstract

Infectious bronchitis virus (IBV) and Newcastle disease virus (NDV) are two poultry pathogens seriously affecting the poultry industry. Here, IBV S1 and the ectodomain of NDV F proteins were separately linked with the trans-membrane and carboxy-terminal domain of IBV S protein (STMCT), composing rS and rF; thus, a novel chimeric infectious bronchitis-Newcastle disease (IB-ND) virus-like particles (VLPs) vaccine containing the rS, rF, and IBV M protein was constructed. Under the transmission electron microscope (TEM), VLPs possessing similar morphology to natural IBV were observed. To evaluate the immunogenicity of chimeric IB-ND VLPs, specific pathogen-free (SPF) chickens were immunized with three increasing doses (50, 75, and 100 μg protein of VLPs). Results of ELISAs detecting IBV and NDV specific antibodies and IL-4 and IFN-γ T cell cytokines indicated that vaccination with chimeric IB-ND VLPs could efficiently induce humoral and cellular immune responses. In the challenge study, chimeric IB-ND VLPs (100 μg protein) provided 100% protection against IBV or NDV virulent challenge from death, and viral RNA levels in tissues and swabs were greatly reduced. Collectively, chimeric IB-ND VLPs are highly immunogenic and could provide complete protection from an IBV or NDV virulent challenge. Chimeric IB-ND VLPs are an appealing vaccine candidate and a promising vaccine platform bearing multivalent antigens.

The Construction and Immunoadjuvant Activities of the Oral Interleukin-17B Expressed by Lactobacillus plantarum NC8 Strain in the Infectious Bronchitis Virus Vaccination of Chickens

“…It has also been confirmed in previous studies that IL-2 can promote the elimination of the Newcastle disease virus (NDV) and can reduce the virus titer of NDV in the blood, spleen, oral secretions, and cloacal excretions [ 48 ]. Moreover, the IBV copies in the tracheas were the highest in these tissues, and a similar result was also reported in our previous IBV challenge assay [ 49 ].…”

Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.

“…IBV is the etiologic agent of infectious bronchitis (IB), which is a highly contagious, acute viral respiratory disease of chickens. IBV has been reported by many researchers all over the world (Jungherr and Terrells, 1948; Jungherr et al, 1956; Fabricant, 1998; Yu et al, 2001; Benyeda et al, 2009; Sjaak De Wit et al, 2011; Westerbeck and Machamer, 2019; Wu et al, 2019). IBV has led to severe losses in the poultry industry (Jordan, 2017), the direct losses are due to highly mortality, poor egg quality, and meat production, and the indirect losses result in increased costs and challenges in IBV prevention (Liang et al, 2019).…”

A Multi-Omics Study of Chicken Infected by Nephropathogenic Infectious Bronchitis Virus

“…NIBV has become the most common IBV strain in the commercial poultry industry. It is a gamma Coronavirus in the Coronavirus family that exhibit strong renal tropism and has become one of the most familiar pathogens that induce visceral gout outbreaks [9,10,11]. Initially, some IBV strains have been called nephropathogenic IBV because the starting respiratory infection was followed by a heavy kidney infection.…”