Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion

Zhang, Shenghui; Sun, Yuliang; Jiang, Dongli; Chen, Tongtong; Liu, Ruihong; Lu, Yilin; Qiao, Liang; Pan, Ying; Li, Yanli

doi:10.1007/s43032-020-00296-2

Cited by 19 publications

(10 citation statements)

References 19 publications

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“…However, the murine reproductive system is very different from that of humans and ethanol-induced uterine damage is clinically rare. The potential mechanism of ethanol-induced endometrial injury cannot completely recapitulate the pathogenesis of clinical IUA, which is the limitation of the animal model [ 41 , 42 ]. To improve the effect of cell therapy, gene therapy with minicircle gene delivery vectors offers one possible way to meet this demand.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects and Repair Mechanism of HGF Gene-Transfected Mesenchymal Stem Cells on Injured Endometrium

Xing

Liu

et al. 2022

Stem Cells International

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There are many studies on the advantages of using mesenchymal stem cells (MSCs) that secrete various paracrine factors for repairing endometrial injury. However, the stability and effectiveness of MSCs require improvement to become a viable therapy. Hepatocyte growth factor (HGF), one of the cytokines secreted by MSCs, promotes vascular repair and mesenchymal to epithelial transformation (MET). Therefore, HGF likely promotes the repair process of the endometrium. We prepared MSCs transfected with the HGF gene to explore its repair effects and mechanism using a damaged endometrium mouse model. HGF gene-transfected MSCs were prepared by electroporation. The transfected MSCs retained their cellular characteristics and significantly increased the expression of HGF ( P < 0.01 ). HGF gene-transfected MSCs helped damaged endometrium to recover its morphological characteristics, improved proliferation and decreased apoptosis of endometrial cells, increased the expression of endometrial vascular growth-related factors, and activated phosphorylated c-Met and AKT in the mouse endometrial damage model ( P < 0.05 ). Compared with normal MSCs, HGF gene-transfected MSCs produced a more significant effect on damaged endometrial epithelium repair by activating the HGF/c-Met and downstream signaling pathways. Our results indicate that HGF gene-transfected MSCs provide an effective and promising tool for injured endometrium therapy.

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Section: Discussionmentioning

confidence: 99%

Therapeutic Effects and Repair Mechanism of HGF Gene-Transfected Mesenchymal Stem Cells on Injured Endometrium

Xing

Liu

et al. 2022

Stem Cells International

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“…A total of 136 mice were used in this study, and the modeling methods were based on previous studies with minor modifications. [ 46 ] The mice were anesthetized with isoflurane and placed in a supine position. An incision of 1 cm was made in the abdomen to expose the uterus.…”

Section: Methodsmentioning

confidence: 99%

“…A murine endometrial damage model was established according to the integrated modification described in previous studies. [46,55] Vaginal pictures were used to identify the sexual cycle of mice (Figure S2, Supporting Information), and mice in the same cycle were selected for model construction and follow-up experiments. The picture flow of the IUA mouse model construction process is described in Figure S3, Supporting Information.…”

Section: Dsc-exos/sah Enhances Endometrial Regeneration In a Murine I...mentioning

confidence: 99%

Incorporation of Decidual Stromal Cells Derived Exosomes in Sodium Alginate Hydrogel as an Innovative Therapeutic Strategy for Advancing Endometrial Regeneration and Reinstating Fertility

Liang,

Shuai,

Zhang

et al. 2024

Adv Healthcare Materials

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Intrauterine adhesion (IUA) stands as a prevalent medical condition characterized by endometrial fibrosis and scar tissue formation within the uterine cavity, resulting in infertility and, in severe cases, recurrent miscarriages. Cell therapy, especially with stem cells, offers an alternative to surgery, but concerns about uncontrolled differentiation and tumorigenicity limit its use. Exosomes, more stable and immunogenicity‐reduced than parent cells, have emerged as a promising avenue for IUA treatment. In this study, a novel approach has been proposed wherein exosomes originating from decidual stromal cells are encapsulated within sodium alginate hydrogel (SAH) scaffolds to repair endometrial damage and restore fertility in a mouse IUA model. Current results demonstrate that in situ injection of decidual stromal cell‐derived exosomes (DSC‐exos)/SAH into the uterine cavity has the capability to induce uterine angiogenesis, initiate mesenchymal‐to‐epithelial transformation (MET), facilitate collagen fiber remodeling and dissolution, promote endometrial regeneration, enhance endometrial receptivity and contribute to the recovery of fertility. RNA sequencing and advanced bioinformatics analysis reveal miRNA enrichment in exosomes, potentially supporting endometrial repair. This finding elucidates how DSC‐exos/SAH mechanistically fosters collagen ablation, endometrium regeneration, and fertility recovery, holding the potential to introduce a novel IUA treatment and offering invaluable insights into the realm of regenerative medicine.This article is protected by copyright. All rights reserved

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“…Since the various severity of the endometrial injury is dependent on the retention duration of ethanol in the uterine horns, 95% alcohol was perfused into either side uterine horn of rabbits for 5 or 10 min, then extracted out, and slowly rinsed with saline to remove residual ethanol, while the contralateral uterine horn was perfused with the equal volume of saline. The 5 min group was defined as a model with mild endometrial injury, and the 10 min group was defined as a model with severe endometrial injury, while their contralateral uterine horn was defined as health endometrium, respectively [24]. 20 days after treatment, OCT/US integrated imaging was performed in each rabbit model (both uterine horns).…”

Section: Establishment Of Endometrial Injury Modelmentioning

confidence: 99%

Imaging biomarker for quantitative analysis of endometrial injury based on optical coherence tomography/ultrasound integrated imaging mode

Zhang

Wang

et al. 2023

Journal of Biophotonics

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Precise evaluation of endometrial injury is significant to clinical decision‐making in gynecological disease and assisted reproductive technology. However, there is a lack of assessment methods for endometrium in vivo. In this research, we intend to develop quantitative imaging markers with optical coherence tomography (OCT)/ultrasound (US) integrated imaging system through intrauterine endoscopic imaging. OCT/US integrated imaging system was established as our previous research reported. The endometrial injury model was established and after treatment, OCT/US integrated imaging and uterus biopsy was performed to evaluate the endometrial thickness, number of superficial fold, and intrauterine area. According to the results, three quantitative indexes acquired from OCT/US image and HE staining have the same trend and have a strong relationship with the severity of the endometrial injury. Accordingly, we developed three imaging markers for quantitative analysis of endometrial injury in vivo, which provided a precise mode for endometrium evaluation in clinical practice.This article is protected by copyright. All rights reserved.

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Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion

Cited by 19 publications

References 19 publications

Therapeutic Effects and Repair Mechanism of HGF Gene-Transfected Mesenchymal Stem Cells on Injured Endometrium

Therapeutic Effects and Repair Mechanism of HGF Gene-Transfected Mesenchymal Stem Cells on Injured Endometrium

Incorporation of Decidual Stromal Cells Derived Exosomes in Sodium Alginate Hydrogel as an Innovative Therapeutic Strategy for Advancing Endometrial Regeneration and Reinstating Fertility

Imaging biomarker for quantitative analysis of endometrial injury based on optical coherence tomography/ultrasound integrated imaging mode

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