Construction and study of leaf rust-resistant common wheat lines with translocations of Aegilops speltoides Tausch. Genetic material

Адонина, И. Г.; Petrash, N. V.; Timonova, E. M.; Khristov, Yu. A.; Салина, Е. А.

doi:10.1134/s1022795412020020

Cited by 21 publications

(12 citation statements)

References 19 publications

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“…Genetic material from Ae. speltoides (SS genome), the putative progenitor of the B and G genomes of polyploid wheat, was successfully transferred to all three genomes of common wheat, but especially, as expected, in the chromosome of the B-genome [60,61].…”

Section: Chromosomal Rearrangement During Wheat Breedingsupporting

confidence: 59%

Cytogenetics in the Study of Chromosomal Rearrangement during Wheat Evolution and Breeding

Салина¹,

Адонина²

2019

Cytogenetics - Past, Present and Further Perspectives

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Cytogenetic methods such as chromosome banding and in situ hybridization remain relevant in the post-genomic era, especially for allopolyploid species where genome duplication in some cases makes it difficult to assess the reorganization of chromosomes during evolution. In this review, we give a brief description of cytogenetic methods for the analysis of homoeological chromosomes in cereals. Emphasis is placed on the development of methods for the study of polyploid wheat and its progenitors and on tandem repeats and retrotransposons as markers to evaluate chromosome reorganization throughout evolution and breeding. The most effective cytological probes used for the identification of chromosomes in wheat and Triticeae species by fluorescence and genomic in situ hybridization are described. Particular attention is paid to ribosomal genes used as markers in phylogenetic studies and for chromosome identification. Utility of these cytogenetic methods in the evaluation of breeding lines is demonstrated. A strategy for cytological analysis of wheat hybrids according to the degree of relationships between the species involved in crosses is also discussed.

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Section: Chromosomal Rearrangement During Wheat Breedingsupporting

confidence: 59%

Cytogenetics in the Study of Chromosomal Rearrangement during Wheat Evolution and Breeding

Салина¹,

Адонина²

2019

Cytogenetics - Past, Present and Further Perspectives

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“…The original line 73/00 i possesses a group resistance to a spectrum of fungal diseases (see Table 1). But only the obtained line with translocation into 5B chromosome had the maximum resistance to leaf rust (Adonina et al, 2012). Earlier, in this chromosome the loci were found responsible for the high protein and gluten content in grain (Gonzalez-Hernandez et al, 2004;Pshenichnikova et al, 2012).…”

Section: Discussionmentioning

confidence: 95%

“…speltoides, introgressed into the genome of bread wheat cultivar Rodina, on technological characteristics of grain and flour. The transfer of alien genetic material was confirmed by genetic, cytogenetic, and molecular methods (Salina et al, 2001;Adonina et al, 2004Adonina et al, , 2012Gajnullin et al, 2007). Long-term studies have shown that the lines carry genes for resistance to fungal diseases; some of them were identified (see Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…In the work of Adonina et al (2012), using fluorescent hybridization with the Spelt1 and pSc119.2 probes in combination with microsatellite markers it was shown that the line 73/00 i carries translocations into the short arm of chromosome 1B and the long arm of chromosomes 5B and 6B. Subsequently, these translocations were transferred to separate lines based on the Rodina cultivar (Adonina et al, 2012). The original line 73/00 i possesses a group resistance to a spectrum of fungal diseases (see Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic diversity of bread wheat lines with introgressions from the diploid cereal Aegilops speltoides for technological properties of grain and f lour

2020

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The creation of varieties adapted to changing environmental conditions, resistant to various pathogens, and satisfying various grain purposes is impossible without using the genetic diversity of wheat. One of the ways to expand the genetic diversity of wheat is to introduce new variants of genes from the genetic pool of congeners and wild relatives into the genotypes of existing varieties. In this study, we used 10 lines from the Arsenal collection created on the genetic basis of the spring variety ‘Rodina’ and the diploid species Aegilops speltoides in the Federal Research Center “Nemchinovka” in 1994. The lines were previously characterized for the presence of translocations and chromosomal rearrangements cytologically and using molecular markers. Technological analyses were performed on grain obtained in Western Siberia and Moscow region. The aim of this study was to establish the possibilities of expanding the phenotypic diversity for technological properties of grain and flour as a result of such hybridization of bread wheat and the diploid cereal Aegilops speltoides. The variety ‘Rodina’ forms a vitreous grain with a high gluten content in Siberia, but has low physical properties of flour and dough. Five derived lines were found to have significantly higher protein and gluten content in grain. The highest values under both growing conditions were found in lines 73/00i, 82/00i, and 84/00i. Two lines (69/00i and 76/00i) showed a high flour strength and dough elasticity, characterizing the lines as strong and valuable in quality. These lines can be used for baking bread. Line 82/00i inherited from Ae. speltoides a soft-grain endosperm, which indicates the introgression of the Ha-Sp gene, homoeoallelic to the Ha gene of bread wheat, into ‘Rodina’. Flour of this line is suitable for the manufacture of confectionery without the use of technological additives. The lines generally retained their characteristics in different growing conditions. They can be attracted as donors of new alleles of genes that determine the technological properties of grain and resistance to biotic stresses.

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“…These studies isolated com mon wheat cultivars with potential for cold resistance and higher quality of wheat flour [134,135]. In addi tion, assessments were made in regard to positive and negative impacts of foreign genetic material on pro ductivity indicators.…”

Section: Immunity and Ecological Plasticitymentioning

confidence: 99%

Genetics and genomics of wheat: Storage proteins, ecological plasticity, and immunity

Novoselskaya-Dragovich

2015

Russ J Genet

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Recent advances in genetics and genetic research methods made it possible to explain the large polymorphism observed among storage proteins of wheat grain (gliadins and glutenins), to determine their genetic control mechanism, and to develop a system for the identification of wheat genotypes on the basis of multiple allelism. This system has extremely high sensitivity and efficiency, which makes it possible to con duct studies to determine the purity and authenticity of wheat cultivars, the dynamics of alleles diversity in time and space, the phylogenetics, etc., through the use of an extensive database on the allelic composition of gliadin loci. An investigation of the molecular structure of genes controlling the synthesis of storage pro teins and their organization on chromosomes, as well as an analysis of wheat genome structure, revealed the molecular mechanisms of variability in the wheat genome and its reorganization in response to changes in environmental conditions and cultivation technologies. The multilevel genetic system of protection against pathogens and adverse environmental factors that developed in the course of wheat evolution continues to astound researchers' imagination with new resistance genes and novel types of antimicrobial peptides having been discovered and sequenced in recent years and the diversity of their structures and mechanisms of action in response to pathogens. An analysis of nucleotide sequences involved in wheat domestication, namely, those that define ecological plasticity, i. e. the type of plant development (Vrn genes), and those responsible for spikelet traits (Q genes), which ensured the successful cultivation of wheat by humans, revealed that the basis for these features are specific mutations.

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Construction and study of leaf rust-resistant common wheat lines with translocations of Aegilops speltoides Tausch. Genetic material

Cited by 21 publications

References 19 publications

Cytogenetics in the Study of Chromosomal Rearrangement during Wheat Evolution and Breeding

Cytogenetics in the Study of Chromosomal Rearrangement during Wheat Evolution and Breeding

Phenotypic diversity of bread wheat lines with introgressions from the diploid cereal Aegilops speltoides for technological properties of grain and f lour

Genetics and genomics of wheat: Storage proteins, ecological plasticity, and immunity

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