Construction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide<sub>2-14</sub>of bovine ribonuclease A

Harvey, Clifford; Olson, Kenneth; Czekala, Alice De; Nussbaum, A. L.

doi:10.1093/nar/2.11.2007

Cited by 9 publications

(1 citation statement)

References 37 publications

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“…Alex's foresight on the impact of molecular biology was far ahead of most at Hoffman-Roche. He chemically synthesized DNA encoding the terminal redundancy of phage λDNA (270) as well as the S peptide of ribonuclease A (271). I consulted with Hoffman-Roche for several years, but I could do little to convince the senior group that there might be a future in recombinant DNA.…”

Section: Hoffman-rochementioning

confidence: 99%

Untitled

2015

Annu. Rev. Biochem.

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I spent my childhood and adolescence in North and South Carolina, attended Duke University, and then entered Duke Medical School. One year in the laboratory of George Schwert in the biochemistry department kindled my interest in biochemistry. After one year of residency on the medical service of Duke Hospital, chaired by Eugene Stead, I joined the group of Arthur Kornberg at Stanford Medical School as a postdoctoral fellow. Two years later I accepted a faculty position at Harvard Medical School, where I remain today. During these 50 years, together with an outstanding group of students, postdoctoral fellows, and collaborators, I have pursued studies on DNA replication. I have experienced the excitement of discovering a number of important enzymes in DNA replication that, in turn, triggered an interest in the dynamics of a replisome. My associations with industry have been stimulating and fostered new friendships. I could not have chosen a better career.

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Section: Hoffman-rochementioning

confidence: 99%

Untitled

2015

Annu. Rev. Biochem.

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Synthese eines Strukturgens für das Peptidhormon Angiotensin II, Teil 9. Ligation der Teilsequenzen zum kompletten Doppelstrang

Blöcker

Frank

Köster

1978

Justus Liebigs Ann. Chem.

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Die Ligation der sieben Teilsequenzen+zum kompletten 33 Basenpaare umfassenden Doppelstrang einer Angiotensin-11-DNS mit Hilfe von T4 Polynucleotidligase wird beschrieben. Die Ligationsreaktion wird durch einen rnodifizierten Norittest verbunden mit einem Abbau von [y3*P]ATP mit Anorganischer Pyrophosphatase aus Backerhefe verfolgt. Diese neue Analysentechnik macht eine zeitraubende und verlustreiche Reinigung der mit T4 Polynucleotidkinase und [y3*P]ATP phosphorylierten Teilsequenzen uberfliissig und erlaubt es, die DNS-Synthese mehrstufig und ohne Zwischenreinigung durchzufuhren. Synthesis of a Structural Gene for the Peptide Hormone Angiotensin 11, Part 9l). -Ligation of the Partial Sequences to the Complete DuplexLigation of the seven partial sequences by T4 polynucleotide ligase to give the total duplex of an angiotensin I1 DNA with 33 base pairs is described. The ligation reaction was monitored by a modified Norit assay combined with the degradation of [Y-~'P]ATP by inorganic pyrophosphatase from baker's yeast. This new analytical technique makes it unnecessary to purify the segments after phosphorylation with T4 polynucleotide kinase and [y3'P]ATP by time-consuming and yielddecreasing methods. Moreover, this technique permits the synthesis of double helical DNA in a multiple step reaction without any intermediate purification. EinleitungNachdem in den voranstehenden Arbeiten (Teile 1 -8 dieser Reihe) die Synthese der sieben Teilsequenzen und deren Sequenzierung beschrieben wurden, wird in dieser Verdffentlichung uber ihre Kopplung zurn totalen Doppelstrang einer DNS fur das Peptidhorrnon Angiotensin I1 (Abbildung 1) berichtet. Eine Kurzmitteilung hieriiber ist bereits erschienen2). Anschlierjend wurde iiber die Synthese der Lactose-Operator-DNS3s4), einer DNS fur den Tyrosin-tRNS-Vorlaidfer aus E. c01i5), uber eine DNS fur Korrespondenz bitte an diesen Autor richten. H. Blocker und H.

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Chemische DNA‐Synthesen

Köster¹

1979

Nachr. Chem. Tech. Lab.

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Construction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide_2-14of bovine ribonuclease A

Cited by 9 publications

References 37 publications

Untitled

Untitled

Synthese eines Strukturgens für das Peptidhormon Angiotensin II, Teil 9. Ligation der Teilsequenzen zum kompletten Doppelstrang

Chemische DNA‐Synthesen

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Construction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide2-14of bovine ribonuclease A

Cited by 9 publications

References 37 publications

Untitled

Untitled

Synthese eines Strukturgens für das Peptidhormon Angiotensin II, Teil 9. Ligation der Teilsequenzen zum kompletten Doppelstrang

Chemische DNA‐Synthesen

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Product

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Construction of a double-stranded deoxyribonucleotide sequence of 45 base pairs designed to code for S-peptide_2-14of bovine ribonuclease A