Construction of a myocardial patch with mesenchymal stem cells and poly(CL-co-TOSUO)/collagen scaffolds for myocardial infarction repair by coaxial electrospinning

Abstract

Stem cell cardiac patches have shown promising future for myocardium infarction (MI) therapy, but the characteristics of cardiac pulsation and tissue orientation lead to challenges in the design of cardiac...

Electrospun fibers modified with polydopamine for enhancing human mesenchymal stem cell culture

“…MSCs cultured on PCL fibers coated with PDA and PDA/RGD exhibited a more stretched and wellspread morphology. MSCs are considered to have good cell adhesion and a widespread distribution when they have spindle-like shapes or multipolar morphologies [ 27,38]. The polygonal cell morphology indicates that contact point formation between the cell and matrix was more stimulated [27], and we concluded that the PDA and PDA/RGD coatings enhanced the proliferation and viability of MSCs by promoting MSCs-to-PCL fibers surface interactions.…”

“…phenotypes of their surface markers, which are known to be positive for CD90 and CD73, and negative for CD34 [2,27]. These expression markers can also be used as standards for evaluating MSCs properties such as multilineage potential [27,38]. The expression levels of CD90 were 74.8% in pristine PCL fibers, 81.7% in PDA-coated PCL fibers, and 84.5% in PDA/RGD-coated PCL fibers.…”

Recent Advances in Electrospinning Techniques for Precise Medicine

“…Besides the hybridization of multiple types of materials, heterogenous fibers (bioactive materials/synthetic polymers) and orchestrated fibers produced by advanced electrospinning techniques may achieve an identical objective by encouraging cellular functions and guiding cellular arrangement/migration, respectively. Despite the few reported studies, the structures composed of core–shell or Janus fibers, as well as aligned fibers, have been utilized as biofunctional scaffolds for tissue regeneration and repairs (e.g., blood vessel grafts [ 175 , 176 ], skin grafts [ 177 , 178 ], and cardiac patch [ 179 , 180 ]), demonstrating their potential to be advanced membranes in OOCs devices.…”

Comparative effectiveness of myocardial patches and intramyocardial injections in treating myocardial infarction with a MitoQ/hydrogel system

“…14,17 In contrast, hydrogel patches reduce the risk of surgical invasiveness by utilizing polymeric materials capable of undergoing a sol–gel transition or incorporating memory-smart-responsive materials to load active substances into the hydrogel, thus promoting adhesion to and treatment of the infarcted area. 18,19 In comparison to hydrogel intramyocardial injections, the drug delivery of hydrogel patches necessitates traversing a dense myocardial layer, meeting increased wet adhesion requirements at the gel-solid interface, as well as possessing a higher level of surgical manipulation skills and experience. 18,20,21 Nevertheless, further clarification is required on the comparative effectiveness between the two therapeutic modalities: intramyocardial injections and hydrogel patches.…”

“…18,19 In comparison to hydrogel intramyocardial injections, the drug delivery of hydrogel patches necessitates traversing a dense myocardial layer, meeting increased wet adhesion requirements at the gel-solid interface, as well as possessing a higher level of surgical manipulation skills and experience. 18,20,21 Nevertheless, further clarification is required on the comparative effectiveness between the two therapeutic modalities: intramyocardial injections and hydrogel patches.…”