Construction of a Prognostic Evaluation Model for Stomach Adenocarcinoma on the Basis of Immune-Related lncRNAs

Xu, Chaobo; Chen, Zheng-Wei; Pan, Xiaoming; Liu, Ming; Cheng, Guoxiong; Li, Jiaxin; Mei, Yijun

doi:10.1007/s12010-022-04098-x

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…The tumor microenvironment is closely related to the progression and prognosis of cancer patients [24]. After detecting the infiltration level of immune cells in the TME of each STAD patient using ssGSEA, immune infiltration and immunocompetence differences between the two risk groups were also compared.…”

Section: Differential Analysis Of the Tumor Microenvironmentmentioning

confidence: 99%

A prognostic model for STAD based on hypoxia- and immune-related genes

Wang,

Zhang,

et al. 2023

Arch Med Sci

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IntroductionFeatured with vast heterogeneity, gastric cancer (GC) is one of the leading causes of cancer-related deaths. A specific prognostic model is necessary for the improvement of clinical treatment strategies. Hypoxia is a common feature in the tumor microenvironment that promotes tumor progression. However, the current evaluation of hypoxic tumor immune microenvironment in GC is still inadequate.Material and methodsWith sequence data and single nucleotide variants data obtained from The Cancer Genome Atlas-STAD dataset as well as hypoxia- and immune-related genes acquired from MsigDB and ImmPort, a hypoxia-immune-based gene signature of gastric adenocarcinoma (STAD) was built by Cox regression analysis. Riskscore could be used as an independent prognostic factor.ResultsReceiver operating characteristic curve and survival curve showed the accuracy of the model. Pearson correlation analysis showed that DUSP1, one of the hypoxia- and immune-related feature genes, was positively correlated with immune cell scores and immune-related function scores. In addition, low-risk group peers were found to be in higher immune infiltration status and had higher immunophenoscore as demonstrated by single-sample GSEA, indicating better response to immune checkpoint inhibitors (ICI) treatment among the low-risk group. q-PCR results showed that DUSP1, IGFBP1, CGB5, GPC3 and EGF were significantly highly expressed in STAD cells, while FAM3D and FGF8 were significantly down-regulated.ConclusionsOverall, our study not only paves the way for future studies focusing on hypoxia and immune microenvironment but also improves STAD patient’s prognosis and their response to immunotherapy.

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Section: Differential Analysis Of the Tumor Microenvironmentmentioning

confidence: 99%

A prognostic model for STAD based on hypoxia- and immune-related genes

Wang,

Zhang,

et al. 2023

Arch Med Sci

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“…The probe of the analyte also impacts the analytical performance and is an important factor for immunoassays of detectable signals. Currently, chemiluminescence (CL) reactions are catalyzed using N-(4-aminobutyl)-N-(ethylisoluminol) or luminol as a luminescence reagent [32][33][34][35][36][37]. As a novel luminescent probe with high e ciency, NSP-DMAE-NHS demonstrates a much higher e ciency [38], improved selectivity, and enhanced emission intensity for quantitative analysis [39].…”

Section: Introductionmentioning

confidence: 99%

Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips

Wang,

He,

2024

Appl Biochem Biotechnol

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Recent studies have shown that almost half of all cancers occur due to DNA damage. For the early diagnosis of cancer, a highly sensitized and swift identi cation for TP53 is needed since the corresponding TP53 protein is effectively recognized as "the guardian of the genome." To improve the detection sensitivity, numerous analytical methods were previously used for the determination of the TP53 protein, including denaturing high-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA). Currently, immunochromatographic tests (ICTS) that are simple to use, stable over time, and show low interference are regarded as valuable tools for the quick screening of food and environmental monitoring along with clinical diagnosis. ICTS often have limited sensitivity even if a variety of novel reporters possessing optimum photostability and improved brightness are used as the signal-intensity reporters. Compared with N-(4-aminobutyl)-N-(ethylisoluminol) or luminol, a novel luminescent probe, 2',6'-diMethyl-4'-(N-succiniMidyloxycarbonyl) phenyl-10-sulfopropylacridiniuM-9carboxylate (NSP-DMAE-NHS) has achieved a much higher e ciency, improvement in the biosensor's performance, and ampli cation of the signal without causing any damage to the biomolecule in terms of its biochemical activity. In this study, the reagent strip method was initially used to detect TP53 fusion protein by combining the advantages of NSP-DMAE-NHS and immunochromatography. In our experiment, the control and study lines on the strips were immobilized through HRP-conjugated goat antirabbit IgG and TP53 antigen, respectively. The optimized concentration of the anti-TP53 antibody-NSP-DMAE-NHS immunoconjugates was then added to the TP53 antigen samples. After, the test strips were inserted and left in the aforementioned buffer solution for an additional 20 minutes. Finally, a lab made luminous measurement device was used to analyze the corresponding control and study lines on the strips. Under optimized conditions, this method was found to be ultrasensitive, with a wide range of linear responses from 0.0008 ng mL -1 to 1 µg mL -1 and a limit of detection of 0.0008 ng mL -1 (0.013 pM). Thus, a novel competitive chemiluminescent assay based on reagent strips was established for the determination of the TP53 fusion proteins. The strategy has potential applications for ultrasensitive detection in the early diagnosis of cancer.

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