Construction of a reverse genetic system for porcine astrovirus

Qin, Yifeng; Fang, Qingli; Huan, Liu; Ji, Chengyuan; Chen, Ying; Ouyang, Kang; Wei, Zuzhang; Huang, Weijian

doi:10.1007/s00705-018-3771-4

Cited by 20 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PK15 cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with antibiotics (100 units/mL of penicillin and 100 units/mL of streptomycin) and 10% fetal bovine serum (FBS) (Gibco). PAstV type 1 strain, named as PAstV1-GX1 (GenBank accession: KF787112), was isolated from a PAstV positive fecal sample from a diarrheal pig in a farm in Nanning, Guangxi province [30]. The PAstV1-GX1 strain was stably passaged in PK-15 cells and cultured in DMEM containing 0.5 µg/mL tosylphenylalanine chloromethyl ketone (TPCK)-treated trypsin.…”

Section: Viruses and Cellsmentioning

confidence: 99%

Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

Fang

Wang

Liu

et al. 2019

Viruses

Self Cite

View full text Add to dashboard Cite

Astroviral infection is considered to be one of the causes of mammalian diarrheal diseases. It has been shown that astrovirus infections cause varying degrees of diarrhea in turkeys and mice. However, the pathogenesis of porcine astrovirus is unknown. In this study, the virulence of a cytopathic porcine astrovirus (PAstV) strain (PAstV1-GX1) isolated from the PK-15 cell line was tested using seven-day-old nursing piglets. The results showed that PAstV1-GX1 infection could cause mild diarrhea, growth retardation, and damage of the villi of the small intestinal mucosa. However, all the above symptoms could be restored within 7 to 10days post inoculation (dpi). To evaluate the innate immunity response of PAstV in vivo, the alteration of inflammatory cytokine expression in piglets infected with PAstV1-GX1 was determined using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The mRNA expression levels of the IFNβ and ISG54 were found to be significantly elevated in virus-infected piglets. In contrast, expression of IFNλ was downregulated in piglets infected with PAstV1-GX1. In addition, the mRNA expression of the tight junction protein 1 and 2 and zonula occludin 1, which are associated with the intestinal barrier permeability, were affected after PAstV1 infection. The present study adds to our understanding of the pathogenic mechanism of PAstV and has established an animal model for further study of pig astrovirus infection.

show abstract

Section: Viruses and Cellsmentioning

confidence: 99%

Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

Fang

Wang

Liu

et al. 2019

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…Astroviruses (AstVs) are a single-stranded, positive-sense RNA viruses with a genome of approximately 6.8 to over 7.9 kb [ 1 ], consisting of a 5′-untranslated region (UTR), three open reading frames(ORFs), a 3′UTR and a poly (A) tail [ 2 ]. Currently, Astroviruses are classified into two genera: namely Mamastroviruses (MAstVs) and Avastroviruses (AAstVs) according to the different types of infected hosts [ 3 ]. In 1975, astrovirus was first identified in faeces of infants [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a duck-origin goose astrovirus in China

Wei

Yang

Wang

et al. 2020

Emerging Microbes & Infections

View full text Add to dashboard Cite

In 2019, a new type of infectious disease characterized with haemorrhage and swellings of kidneys, occurred on commercial duck farms in Shandong province, China. Our systematic investigation led to the isolation of an astrovirus, designated AstV-SDTA strain and was isolated from a diseased duckling using LMH cells. Similar clinical symptoms were reproduced by experimental infection using the AstV-SDTA strain. The complete genome sequencing characterization of AstV-SDTA was conducted using next-generation sequencing (NGS) technique on Illumina HiSeq platform, and used polymerase chain reaction method to verify the NGS results for the obtained whole sequences. Phylogenetic analysis revealed that AstV-SDTA strain belongs to a novel goose astrovirus (GoAstV) branch of avian astroviruses, and the nucleotide homology based on the complete genome sequences among AstV-SDTA and other GoAstV strains deposited in Genbank was 97.2-98.8%. Taken together, these results suggest that the cross-species transmission of novel GoAstV between domestic waterfowl is possible. Further surveillance of novel GoAstV in poultry are needed in order to gain a better understanding of both the molecular and evolutionary characteristics of novel GoAstV.

show abstract

“…Astroviruses are currently classified into two genera, Mamastroviruses (MAstVs) and Avastroviruses (AAstVs) 1 . MAstVs mainly infect mammals including human, ovine, bovine, porcine, feline, canine, mink, bat, deer, mouse, sea lion, dolphin etc., whereas AAstVs generally infect aviansuch as turkey, chicken, duck, pigeon, and goose 1,2 .…”

Section: Introductionmentioning

confidence: 99%

“…MAstVs mainly infect mammals including human, ovine, bovine, porcine, feline, canine, mink, bat, deer, mouse, sea lion, dolphin etc., whereas AAstVs generally infect aviansuch as turkey, chicken, duck, pigeon, and goose 1,2 . Notably, genetic variation and cross-species transmission of astrovirusespose the risk for zoonotic infection 2,3 .…”

Section: Introductionmentioning

confidence: 99%

An emerging novel goose astrovirus associated with gosling gout disease, China

Zhang

Ren

et al. 2018

Emerging Microbes & Infections

View full text Add to dashboard Cite

Since the first isolation from human, astroviruses have been detected in many species. Wide host range and occasional cross-transmission of astrovirus pose a risk for zoonotic infection. Here, novel astroviruses were identified from goslings with recent epidemic gout disease in China. A virus, designated as GD, was efficiently isolated from a diseased gosling using LMH cells. Genome of GD amplified using 5′ and 3′ RACE was 7183nt in full length. Sequence analysis revealed the genome of GD was <60.8% homology with others deposited in Genbank. Moreover, GD could be neutralized by goose convalescent sera, and the gout associated symptom in goslings could be reproduced by GD infection. Our data demonstrated the goose astrovirus could be one of the causative agents of the ongoing gosling gout disease in China. The identification of the goose astrovirus not only diversified the astrovirus species, but also broadened the disease patterns caused by astroviruses.

show abstract

Construction of a reverse genetic system for porcine astrovirus

Cited by 20 publications

References 37 publications

Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

Pathogenic Characteristics of a Porcine Astrovirus Strain Isolated in China

Isolation and characterization of a duck-origin goose astrovirus in China

An emerging novel goose astrovirus associated with gosling gout disease, China

Contact Info

Product

Resources

About