Construction of a shuttle vector based on the small cryptic plasmid pJY33 from Weissella cibaria 33

Park, Ji Yeong; Jeong, Sang‐Ho; Deok, Hyun; Lee, Jae Yong; Liu, Xiaoming; Cho, Min Jeong; Lee, Kang Wook; Kim, Jeong Hwan

doi:10.1016/j.plasmid.2015.03.008

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…Description Reference This study using primer pair: ErmC-F (5′-CCCTCTAGACTTATCGGATAATAA-3′, XbaI site underlined) and ErmC-R (5′-GGGGGATCCACAAAAAATAGGCAC-3′, BamHI site underlined) [11]. The amplified ermC was inserted into pMJ32 at XbaI and BamHI sites.…”

Section: Bacteria and Plasmidsmentioning

confidence: 99%

“…The copy number of pMJ32E in T. halophilus 31 was qualitatively compared with those of pSJE (a theta-type plasmid) in Levilavtobacillus brevis 2.14 [10] and pJY33E (a RCR-plasmid) in L. brevis 2.14 [11]. Three cultures were grown in MRS broth (10 ml) under the same conditions.…”

Section: Stability Of Pmj32e In T Halophilus 31mentioning

confidence: 99%

“…Plasmids from LAB such as Lactobacillus, Leuconostoc, and Weissella genera have been used as the frames for cloning vectors intended for the introduction and expression of genes in LAB [9][10][11]. However, few studies have been conducted on the plasmids from Tetragenococcus species, and few vectors have been constructed based on the plasmids from Tetragenococcus species Construction of cloning vectors for Tetragenococcus species will be helpful not only for the basic studies on the genetics of Tetragenococcus species but also for the genetic engineering of Tetragenococcus species A 8.7 kb cryptic plasmid (pUCL287) from T. halophilus ATCC33315 was characterized and used for vector construction.…”

Section: Introductionmentioning

confidence: 99%

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Construction of a Novel Shuttle Vector for Tetragenococcus species based on a Cryptic Plasmid from Tetragenococcus halophilus

Kim

Kang

et al. 2022

J. Microbiol. Biotechnol.

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A cryptic plasmid (pTH32) was characterized from Tetragenococcus halophilus 32, an isolate from jeotgal, Korean traditional fermented seafood. pTH32 is 3,198 bp in size with G+C content of 35.84%, and contains 4 open reading frames (ORFs). orf1 and orf2 are 456 bp and 273 bp in size, respectively, and their translation products showed 65.16% and 69.35% similarities with RepB family plasmid replication initiators, respectively, suggesting the rolling-circle replication (RCR) mode of pTH32. orf3 and orf4 encodes putative hypothetical protein of 186 and 76 amino acids, respectively. A novel Tetragenococcus-Escherichia coli shuttle vector, pMJ32E (7.3 kb, Em r ), was constructed by ligation of pTH32 with pBluescript II KS(+) and an erythromycin resistance gene (ErmC). pMJ32E successfully replicated in Enterococcus faecalis 29212 and T. halophilus 31 but not in other LAB species. A pepA gene, encoding aminopeptidase A (PepA) from T. halophilus CY54, was successfully expressed in T. halophilus 31 using pMJ32E. The transformant (TF) showed higher PepA activity (49.8 U/mg protein) than T. halophilus 31 cell (control). When T. halophilus 31 TF was subculturd in MRS broth without antibiotic at 48 h intervals, 53.8% of cells retained pMJ32E after 96 h, and only 2.4% of cells retained pMJ32E after 14 days, supporting the RCR mode of pTH32. pMJ32E could be useful for the genetic engineering of Tetragenococcus and Enterococcus species.

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Section: Bacteria and Plasmidsmentioning

confidence: 99%

Section: Stability Of Pmj32e In T Halophilus 31mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction of a Novel Shuttle Vector for Tetragenococcus species based on a Cryptic Plasmid from Tetragenococcus halophilus

Kim

Kang

et al. 2022

J. Microbiol. Biotechnol.

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“…Efficient gene expression systems such as NICE (nisin inducible gene expression) system are also available for the overproduction of important metabolites in LAB [8]. Plasmids inherent among many LAB species serve as the frames for the construction of cloning vectors for LAB, and many of them are small cryptic plasmids [9,10].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240

Lê¹,

Kim²,

Jeon³

et al. 2022

Kor. J. Microbiol. Biotechnol

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Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria-Escherichia coli shuttle vectors.

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Characterization of the cryptic plasmid pOfk55 from Legionella pneumophila and construction of a pOfk55-derived shuttle vector

Nishida

Watanabe

Tachibana

et al. 2017

Plasmid

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Construction of a shuttle vector based on the small cryptic plasmid pJY33 from Weissella cibaria 33

Cited by 4 publications

References 31 publications

Construction of a Novel Shuttle Vector for Tetragenococcus species based on a Cryptic Plasmid from Tetragenococcus halophilus

Construction of a Novel Shuttle Vector for Tetragenococcus species based on a Cryptic Plasmid from Tetragenococcus halophilus

Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240

Characterization of the cryptic plasmid pOfk55 from Legionella pneumophila and construction of a pOfk55-derived shuttle vector

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