Construction of adipose tissue using a silica expander capsule and cell sheet-assembled of decellularized adipose tissue

Zhu, Zhu; Yuan, Zhaoqi; Guo, Linxiumei; Nurzat, Yeltai; Xu, Heng; Zhang, Yixin

doi:10.1016/j.actbio.2021.12.018

Cited by 9 publications

(6 citation statements)

References 61 publications

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“…DAT was prepared according to previous report 43 . Briefly, subcutaneous fat harvested via liposuction from donors at the Wuhan Union hospital and subjected to three freeze–thaw cycles.…”

Section: Methodsmentioning

confidence: 99%

Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

Tang

Zeng

et al. 2023

Sci Rep

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Acellular matrix is a commonly used biomaterial in the field of biomedical engineering and revascularization is the key process to affect the effect of acellular matrix on tissue regeneration. The application of bioactive factors related to angiogenesis has been popular in the regulation of revascularization, but the immune system clearance, uncontrollable systemic reactions, and other factors make this method face challenges. Recent reports showed that engineered cells into nanovesicles can reorganize cell membranes and encapsulate cellular active factors, extending the in vitro preservation of cytokines. However, the problems of exogenous biological contamination and tumorigenicity restricted the clinical transformation and wide application of this method. Here, we for the first time engineer stromal vascular fraction (SVF) which is extracted from fat into nanovesicles (SVF-EVs) for angiogenesis in the acellular matrix. SVF-EVs not only promote the migration of vascular endothelial cells in vitro, but also facilitate the lipogenic differentiation of mesenchymal stem cells. In vivo, SVF-EVs enhanced the retention of decellularized adipose tissue after transplanting to the subcutaneous area of nude mice. Immunofluorescence staining further showed that SVF-EVs promoted the formation of vascular networks with large lumen diameter in the grafted acellular matrix, accompanied by adipocyte regeneration peripherally. These findings reveal that SVF-EVs can be a viable method for accelerating revascularization in acellular matrix, and this process of squeezing tissue into nanovesicles shows the potential for rapid clinical transformation.

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“…DAT was prepared according to previous report 43 . Briefly, subcutaneous fat harvested via liposuction from donors at the Wuhan Union hospital and subjected to three freeze–thaw cycles.…”

Section: Methodsmentioning

confidence: 99%

Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

Tang

Zeng

et al. 2023

Sci Rep

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“…More recently, decellularized ECM scaffolds from different tissues, including AT, have been tested [235][236][237]. Characterized by high biocompatibility, AT scaffolds are also composed of all the acellular components of the AT naturally resembling the best environment for cell recruitment, attachment, proliferation and differentiation [237][238][239]. Different in vivo studies mixed AT scaffolds with methylcellulose, methacrylated glycol, chitosan, or chondroitin sulfate, forming injectable hydrogels.…”

Section: Adipose Tissue Engineeringmentioning

confidence: 99%

The impact of adipokines on vascular networks in adipose tissue

Vliora

Ravelli²,

Grillo³

et al. 2023

Cytokine & Growth Factor Reviews

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“…However, these adipose sheets did not contain a vascular component. Zhu et al recently isolated human DAT and ASCs to combine with HUVECs and form prevascularized AT cell sheets [303]. Additionally, they stimulated adipo-and angiogenesis by adding exogenous CC chemokine ligand 2.…”

Section: Cell Sheetsmentioning

confidence: 99%

Vascularized adipose tissue engineering: moving towards soft tissue reconstruction

Peirsman,

Nguyen,

Van Waeyenberge

et al. 2023

Biofabrication

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Soft tissue defects are a common clinical challenge mostly caused by trauma, congenital anomalies and oncological surgery. Current soft tissue reconstruction (STR) options include synthetic materials (fillers and implants) and autologous adipose tissue transplantation through flap surgery and/or lipotransfer. Both reconstructive options hold important disadvantages to which vascularized adipose tissue engineering (VATE) strategies could offer solutions. In this review, we first summarized pivotal characteristics of functional adipose tissue (FAT) such as the structure, function, cell types, development and extracellular matrix (ECM). Next, we discussed relevant cell sources and how they are applied in different state-of-the-art VATE techniques. Herein, biomaterial scaffolds and hydrogels, ECMs, spheroids, organoids, cell sheets, 3D bioprinting and microfluidics are overviewed. Also, we included extracellular vesicles and emphasized their potential role in VATE. Lastly, current challenges and future perspectives in VATE are pointed out to help to pave the road towards clinical applications.

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Construction of adipose tissue using a silica expander capsule and cell sheet-assembled of decellularized adipose tissue

Cited by 9 publications

References 61 publications

Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

Engineered nanovesicles from stromal vascular fraction promote angiogenesis and adipogenesis inside decellularized adipose tissue through encapsulating growth factors

The impact of adipokines on vascular networks in adipose tissue

Vascularized adipose tissue engineering: moving towards soft tissue reconstruction

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