Construction of an impedimetric immunosensor for label-free detecting carbofuran residual in agricultural and environmental samples

Liu, Lin; Xu, Dan; Yinyue, Hu; Liu, Shuzhao; Wei, Honglin; Zheng, Jian; Wang, Guoxiu; Hu, Xiaoyun; Wang, Chengyin

doi:10.1016/j.foodcont.2015.01.009

Cited by 38 publications

(13 citation statements)

References 33 publications

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“…Table 1 summarizes the strategies and features of the electrochemical immunosensors discussed in this section. The most used approach for the label-free and non-competitive pesticides detection was Electrochemical Impedance Spectroscopy (EIS) [ 58 , 59 , 60 , 61 , 62 , 63 ]. In EIS the detection of the analyte is carried out indirectly through the impedimetric response of an electrochemical probe with known and stable electrochemical behavior (e.g., ferrocyanide/ferricyanide redox couple).…”

Section: Immunosensorsmentioning

confidence: 99%

“…Liu et al [ 59 ] developed an impedimetric immunosensor for the sensitive detection of carbofuran by immobilizing Abs on L-cysteine modified gold electrodes. The limit of detection (LOD) obtained was 4.52 × 10 −10 M. This method was successfully applied to detect carbofuran in spiked tomato, cabbage, and lettuce samples with an excellent recovery range 90–106%, demonstrating that the sensor can be applied to the detection of carbofuran in real samples.…”

Section: Immunosensorsmentioning

confidence: 99%

See 1 more Smart Citation

Affinity Sensing Strategies for the Detection of Pesticides in Food

Capoferri

Pelle

Carlo

et al. 2018

Foods

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This is a review of recent affinity-based approaches that detect pesticides in food. The importance of the quantification and monitoring of pesticides is firstly discussed, followed by a description of the different approaches reported in the literature. The different sensing approaches are reported according to the different recognition element used: antibodies, aptamers, or molecularly imprinted polymers. Schemes of detection and the main features of the assays are reported and commented upon. The large number of affinity sensors recently developed and tested on real samples demonstrate that this approach is ready to be validated to monitor the amount of pesticides used in food commodities.

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Section: Immunosensorsmentioning

confidence: 99%

Section: Immunosensorsmentioning

confidence: 99%

Affinity Sensing Strategies for the Detection of Pesticides in Food

Capoferri

Pelle

Carlo

et al. 2018

Foods

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“…Carbofuran is a broad-spectrum insecticide widely used in a variety of agricultural crops to shorten the crop growth period and increase crop yields [1]. Due to its high solubility, carbofuran is easily absorbed by plant roots and transported to various plant organs, especially the edge of leaves [2]. However, carbofuran is highly toxic [3], and its use in agricultural products poses a potential hazard for human health and the environment [4].…”

Section: Introductionmentioning

confidence: 99%

Development of a Simple Pretreatment Immunoassay Based on an Organic Solvent-Tolerant Nanobody for the Detection of Carbofuran in Vegetable and Fruit Samples

et al. 2019

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Nanobodies are one-tenth the size of conventional antibodies and are naturally obtained from the atypical heavy-chain-only antibodies present in camelids. Their small size, high solubility, high stability, and strong resilience to organic solvents facilitate their use as novel analytical reagents in immunochemistry. In this study, specific nanobodies against pesticide carbofuran were isolated and characterized from an immunized library via phage display platform. We further established an indirect competitive enzyme-linked immunosorbent assay (ELISA) using nanobody Nb316 to detect carbofuran in vegetable and fruit samples. The results showed a half-maximal inhibitory concentration (IC50) of 7.27 ng/mL and a detection limit of 0.65 ng/mL. A simplified sample pretreatment procedure omitting the evaporation of organic solvent was used. The averaged recovery rate of spiked samples ranged between 82.3% and 103.9%, which correlated with that of standard UPLC–MS/MS method. In conclusion, a nanobody with high specificity for carbofuran was characterized, and a nanobody-based sensitive immunoassay for simple and rapid detection of carbofuran in real samples was validated.

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“…Because of its widespread use in agriculture and high toxicity, contamination of vegetables and grains has become imminent, and consequently adverse health effects are an inevitable concern to humans (Gupta, ). To eliminate the hazards, many countries, including China and the United States, have restricted the use of carbofuran in vegetables, fruit trees, herbs, and tea (Liu et al., ). The maximum residue limit (MRL) of carbofuran in vegetables in China is 0.02 mg/kg, with an exception of 0.1 mg/kg in potatoes (Nation Health Commission of the People's Republic of China, ).…”

Section: Introductionmentioning

confidence: 99%

Application of Immunoassays for Rapid Monitor of Carbofuran Residue in Vegetables

Zhao

Tan

Wang

et al. 2019

Journal of Food Science

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Carbofuran residue in vegetables is a concern to human health. Direct competitive enzyme‐linked immunosorbent assay (dcELISA) and dipstick immunoassay were developed in the present study. The dcELISA showed a 50% inhibition concentration (IC50) and working range of 1.3 and 0.2 to 7.5 ng/mL, respectively, while the cutoff value of dipstick immunoassay was 20 ng/mL. Applying the two immunoassays, we achieved the goal of rapid screening of carbofuran residue in commercial vegetables with a simple sample processing method. Among 46 leek, 39 potato, and 39 sweet potato samples, carbofuran residue was detected in 22% of the leek samples, and two samples exceeded the maximum residue limit of China (0.02 mg/kg). In addition, carbofuran residue was found at less than 2.5 ng/g in one potato and one sweet potato samples. The residual level of carbofuran measured by immunoassays agreed well with those determined by ultra‐performance liquid chromatography tandem mass spectrometry. To ensure food safety and human health, it is greatly necessary and meaningful to monitor carbofuran residue in commercial vegetables. Practical Application Rapid monitoring of carbofuran residue in vegetables is very necessary and important for consumers, regulatory agencies, and food industry.

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Construction of an impedimetric immunosensor for label-free detecting carbofuran residual in agricultural and environmental samples

Cited by 38 publications

References 33 publications

Affinity Sensing Strategies for the Detection of Pesticides in Food

Affinity Sensing Strategies for the Detection of Pesticides in Food

Development of a Simple Pretreatment Immunoassay Based on an Organic Solvent-Tolerant Nanobody for the Detection of Carbofuran in Vegetable and Fruit Samples

Application of Immunoassays for Rapid Monitor of Carbofuran Residue in Vegetables

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