2023
DOI: 10.3390/biom13010091
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Construction of Bi-Enzyme Self-Assembly Clusters Based on SpyCatcher/SpyTag for the Efficient Biosynthesis of (R)-Ethyl 2-hydroxy-4-phenylbutyrate

Abstract: Cascade reactions catalyzed by multi-enzyme systems are important in science and industry and can be used to synthesize drugs and nutrients. In this study, two types of macromolecules of bi-enzyme self-assembly clusters (BESCs) consisting of carbonyl reductase (CpCR) and glucose dehydrogenase (GDH) were examined. Stereoselective CpCR and GDH were successfully fused with SpyCatcher and SpyTag, respectively, to obtain four enzyme modules, namely: SpyCatcher-CpCR, SpyCatcher-GDH, SpyTag-CpCR, and SpyTag-GDH, whic… Show more

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Cited by 6 publications
(4 citation statements)
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“…The absorption peak intensity at 910 cm −1 compared to unmodified epoxy LXTE-600 decreased due to the covalent binding of the epoxy group with the Catcher protein [33]. The characteristic peak variation at 1642 cm −1 was due to C=O stretching vibration and N-H stretching and bending vibration in the amide I band, while the absorption peak at 3400 cm −1 was related to hydroxyl or amine group vibration [34]. The results indicated that the functional groups of the fixed epoxy carrier were altered, and the enzyme was successfully fixed on the resin.…”
Section: Characterization Of Immobilized Enzymesmentioning
confidence: 96%
“…The absorption peak intensity at 910 cm −1 compared to unmodified epoxy LXTE-600 decreased due to the covalent binding of the epoxy group with the Catcher protein [33]. The characteristic peak variation at 1642 cm −1 was due to C=O stretching vibration and N-H stretching and bending vibration in the amide I band, while the absorption peak at 3400 cm −1 was related to hydroxyl or amine group vibration [34]. The results indicated that the functional groups of the fixed epoxy carrier were altered, and the enzyme was successfully fixed on the resin.…”
Section: Characterization Of Immobilized Enzymesmentioning
confidence: 96%
“…4 B) Another research group has demonstrated that the multi-enzyme complex based on the SpyTag/SpyCatcher system improves catalytic efficiency (Fig. 4 C) (Wang et al 2023a , b ). The stereoselective carbonyl reductase (CpCR) and glucose dehydrogenase (GDH) successfully fused with SpyCatcher and SpyTag to form two double enzyme self-assembled clusters, named CpCR–SpyCatcher–SpyTag–GDH and GDH–SpyCatcher–SpyTag–CpCR.…”
Section: Strategies Of Enzyme Immobilization Mediated By a Scaffoldmentioning
confidence: 99%
“…The stereoselective carbonyl reductase (CpCR) and glucose dehydrogenase (GDH) successfully fused with SpyCatcher and SpyTag to form two double enzyme self-assembled clusters, named CpCR–SpyCatcher–SpyTag–GDH and GDH–SpyCatcher–SpyTag–CpCR. CpCR–SpyCatcher–SpyTag–GDH showed better activity and efficiently converted ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl(R)2-hydroxy-4-phenylbutanoate ((R)-HPBE), while regenerating NADPH with a unique structure and excellent catalytic activity, improving the catalytic efficiency of the enzyme (Wang et al 2023a , b ). Overall, these examples demonstrate the practicality of the SpyTag/SpyCatcher system in biotechnology.…”
Section: Strategies Of Enzyme Immobilization Mediated By a Scaffoldmentioning
confidence: 99%
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