Construction of ceRNA Network Based on TCGA and UCSC Xena Official Website to Provide Certain Help for Immunotherapy of Thyroid Cancer.

Abstract

Background: Thyroid cancer is the most common endocrine tumor, and papillary thyroid carcinoma is the most common. Commonly used treatment methods include surgery and radiotherapy. As an emerging treatment option in recent years, immunotherapy has played an essential role in the treatment of many tumors, but there are few studies in thyroid cancer. Method: We first downloaded the mRNA and lncRNA data of thyroid cancer patients from the TCGA official website https://portal.gdc.cancer.gov/, and downloaded the miRNA of thyroid cancer patients from UCSC Xena https://xenabrowser.net/ data. Through the tumor microenvironment's immune score, tumor samples are divided into cold tumors and hot tumors. We obtained the differential genes of DEGs, lncRNA and miRNA, and jointly constructed a ceRNA network through differential analysis of the mRNA data of cold and hot tumours. Results: We first assessed the immune infiltration of the tumor microenvironment of patients with thyroid cancer, and divided the samples into cold tumors and hot tumors based on the immune score. A total of 568 up-regulated DEGs and 412 down-regulated DEGs were screened by analysing the difference between hot and cold tumours. Then we conducted a differential analysis of lncRNA and miRNA and obtained 629 differential genes of lncRNA, and 114 differential genes of miRNA. Finally, we constructed a ceRNA network for the acquired differential genes. Five miRNA-centric hubnets, hsa-mir-204, hsa-mir-128, hsa-mir-214, hsa-mir-150 and hsa-mir-338, have been identified. Conclusion: Our research has identified the immune-related mRNA, lncRNA and miRNA DEGs of thyroid cancer, and constructed a ceRNA network. This result is of great significance for exploring immune-related mechanisms of thyroid cancer and provides certain help for immunotherapy of thyroid cancer.