Construction of Efficient Platform Escherichia coli Strains for Polyhydroxyalkanoate Production by Engineering Branched Pathway

Jung, Hye-Rim; Yang, Suyeon; Moon, Yu-Mi; Choi, Taekjib; Song, Hun-Suk; Bhatia, Shashi Kant; Gurav, Ranjit; Kim, Eun-Jung; Kim, Byung‐Gee; Yang, Yung‐Hun

doi:10.3390/polym11030509

Cited by 59 publications

(15 citation statements)

References 42 publications

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“…The residual PHB was measured using GC as previously described with a slight modification [ 17 , 18 , 19 , 20 ]. For analysis, the culture medium was centrifuged at 10,000× g for 10 min to collect the residual PHB films and rinsed with deionized water to remove the cell residue attached to the film.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

et al. 2021

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Having the advantage of eco-friendly decomposition, bioplastics could be used to replace petroleum-based plastics. In particular, poly(3-hydroxybutyrate) (PHB) is one of the most commercialized bioplastics, however, necessitating the introduction of PHB-degrading bacteria for its effective disposal. In this study, Microbulbifer sp. SOL66 (94.18% 16S rRNA with similarity to Microbulbifer hydrolyticus) demonstrated the highest degradation activity among five newly screened Microbulbifer genus strains. Microbulbifer sp. SOL66 showed a rapid degradation yield, reaching 98% in 4 days, as monitored by laboratory scale, gas chromatography-mass spectrometry, scanning electron microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The PHB film was completely degraded within 7 days at 37 °C in the presence of 3% NaCl. When 1% xylose and 0.4% ammonium sulfate were added, the degradation activity increased by 17% and 24%, respectively. In addition, this strain showed biodegradability on pellets of poly(3-hydroxybutyrate-co-4-hydroxybutyrate), as confirmed by weight loss and physical property changes. We confirmed that Microbulbifer sp. SOL66 has a great ability to degrade PHB, and has rarely been reported to date.

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Section: Methodsmentioning

confidence: 99%

Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

et al. 2021

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“…Synthetic biology tools have increasingly been employed to solve scientific and technical challenges in metabolic engineering. In recent years, there have been incremental studies on account of synthetic biology and metabolic engineering technologies to improve the PHA product yields at lower cost and their development in a broad range of microorganisms including Halomonas bluephagenesis (Chen et al, 2016;Meng and Chen, 2018;Zheng et al, 2020), Ralstonia eutropha (Raberg et al, 2018;Bhatia et al, 2019), Pseudomonas putida (Prieto et al, 2016;Weimer et al, 2020), Aeromonas hydrophila (Mozejko-Ciesielska et al, 2019), Haloferax mediterranei (Lu et al, 2008;Lin et al, 2021), and recombinant Escherichia coli (Rahman et al, 2013;Jung et al, 2019).…”

Section: Synthetic Biology and Metabolic Engineering Strategies For Pha Productionmentioning

confidence: 99%

Microbial-Derived Polyhydroxyalkanoate-Based Scaffolds for Bone Tissue Engineering: Biosynthesis, Properties, and Perspectives

Li¹,

Zhang²,

Udduttula³

et al. 2021

Front. Bioeng. Biotechnol.

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Polyhydroxyalkanoates (PHAs) are a class of structurally diverse natural biopolyesters, synthesized by various microbes under unbalanced culture conditions. PHAs as biomedical materials have been fabricated in various forms to apply to tissue engineering for the past years due to their excellent biodegradability, inherent biocompatibility, modifiable mechanical properties, and thermo-processability. However, there remain some bottlenecks in terms of PHA production on a large scale, the purification process, mechanical properties, and biodegradability of PHA, which need to be further resolved. Therefore, scientists are making great efforts via synthetic biology and metabolic engineering tools to improve the properties and the product yields of PHA at a lower cost for the development of various PHA-based scaffold fabrication technologies to widen biomedical applications, especially in bone tissue engineering. This review aims to outline the biosynthesis, structures, properties, and the bone tissue engineering applications of PHA scaffolds with different manufacturing technologies. The latest advances will provide an insight into future outlooks in PHA-based scaffolds for bone tissue engineering.

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“…[ 139 ] Deletion of four non‐essential genes ( adhE, pflb, fnr , and IdhA ) using CRISPR/Cas9 system improved PHA production (from 1.8 to 2.1 g L −1 ) in glucose utilizing recombinant E. coli HR002 while reducing metabolic flux towards by‐products. [ 140 ] Knockout of prpC gene (2‐methylcitrate synthase) through CRISPRi in Halomonas sp. TD01 produced 82.20 ± 3.82 g L −1 PHA from 1 g L −1 propionic acid.…”

Section: Metabolic Engineering Synthetic Biology and Related Strategies For Pha Productionmentioning

confidence: 99%

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

Lhamo

Behera

Mahanty

2021

Biotechnology Journal

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Background Microbial polyhydroxyalkanoates (PHAs) produced using renewable resources could be the best alternative for conventional plastics. Despite their incredible potential, commercial production of PHAs remains very low. Nevertheless, sincere attempts have been made by researchers to improve the yield and economic viability of PHA production by utilizing low‐cost agricultural or industrial wastes. In this context, the use of efficient microbial culture or consortia, adoption of experimental design to trace ideal growth conditions, nutritional requirements, and intervention of metabolic engineering tools have gained significant attention. Purpose and scope This review has been structured to highlight the important microbial sources for PHA production, use of conventional and non‐conventional substrates, product optimization using experimental design, metabolic engineering strategies, and global players in the commercialization of PHA in the past two decades. The challenges about PHA recovery and analysis have also been discussed which possess indirect hurdle while expanding the horizon of PHA‐based bioplastics. Summary Selection of appropriate microorganism and substrate plays a vital role in improving the productivity and characteristics of PHAs. Experimental design‐based bioprocess, use of metabolic engineering tools, and optimal product recovery techniques are invaluable in this dimension. Conclusion Optimization strategies, which are being explored in isolation, need to be logically integrated for the successful commercialization of microbial PHAs.

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Construction of Efficient Platform Escherichia coli Strains for Polyhydroxyalkanoate Production by Engineering Branched Pathway

Cited by 59 publications

References 42 publications

Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

Isolation of Microbulbifer sp. SOL66 with High Polyhydroxyalkanoate-Degrading Activity from the Marine Environment

Microbial-Derived Polyhydroxyalkanoate-Based Scaffolds for Bone Tissue Engineering: Biosynthesis, Properties, and Perspectives

Process optimization, metabolic engineering interventions and commercialization of microbial polyhydroxyalkanoates production – A state‐of‐the art review

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