Construction of glutamate biosensor based on covalent immobilization of glutmate oxidase on polypyrrole nanoparticles/polyaniline modified gold electrode

Batra, Bhawna; Kumari, Smita; Pundir, C.S.

doi:10.1016/j.enzmictec.2014.02.001

Cited by 66 publications

(23 citation statements)

References 41 publications

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“…The biosensor was constructed by the immobilized glutamate dehydrogenase on polypyrrole nanoparticles and the polyaniline composite film on an Au electrode. The biosensor exhibited a rapid response time within 3 sec, excellent sensitivity with a detection limit as 0.1 nM, and a wider linear range from 0.02 to 400 µM [62]. Wang et al developed an acetylcholinesterase (AChE) biosensor based on zinc oxide nanoparticles (ZnO-NP), carboxylic graphene (CGR), and Nafion (NF) hybrid modified glassy carbon electrode (GCE).…”

Section: Biosensormentioning

confidence: 99%

Recent progress in nanobiocatalysis for enzyme immobilization and its application

Min

Yoo

2014

Biotechnol Bioproc E

146

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Recent advances in nanotechnology have provided various nanoscale materials that can be used as support for enzyme immobilization. Nanobiocatalysis integrating the biocatalyst and nanoscale materials is drawing great attention as innovative technology. Nanobiocatalysis could achieve not only a much higher enzyme loading capacity and a significantly enhanced mass transfer efficiency, but also unbelievable stabilization. In this review, we will present and discuss the recent progress in nanobiocatalysis and its applications in the fields of bioelectronics, bioconversion, and proteomics.

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Section: Biosensormentioning

confidence: 99%

Recent progress in nanobiocatalysis for enzyme immobilization and its application

Min

Yoo

2014

Biotechnol Bioproc E

146

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“…Thus, the determination of this compound is extremely important in both clinical and food samples. Various methods have been used to determine glutamate including high performance liquid chromatography (HPLC) ,, capillary electrophoresis , optical ,, and electrochemical techniques . Among the latter methods, a special attention is payed to electrochemical biosensors due to their sensitivity, selectivity and simple operation .…”

Section: Introductionmentioning

confidence: 99%

A Strategy to Employ Polymerised Riboflavin in the Development of Electrochemical Biosensors

et al. 2017

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Electrochemically polymerised riboflavin (PRf) film was applied in development of glutamate and hypoxanthine biosensors using appropriate oxidases as recognition elements. PRf served as an enzyme immobilisation matrix composed of natural monomer and as a redox mediator. The PRf film was deposited on glassy carbon electrode (GCE) or on the laser‐designed microstructures of the chitosan‐graphene oxide (Chit‐GO) film deposited on glass slide, which served as a chip sensor. The redox polymer was stable and efficient mediator, however, Chit‐GO was placed on the top of PRf/GCE in order to increase the response signal. Such an approach allowed to extend the stability of glutamate and hypoxanthine biosensors to at least 1 month and 15 days, respectively. The chip microelectrode was a single use disposable biosensor due to insufficiently good Chit‐GO adhesion on the top of the cover‐glass. The glutamate microbiosensor had extremely high sensitivity of 16 μA cm−2 μmol L−1.

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“…The lowest limit of detection achieved with a glutamate biosensor was fabricated by covalently immobilizing glutamate oxidase onto polypyrrole nanoparticles and polyaniline composite film (PPyNPs/PANI) [20]. Cyclic voltammetry was used to co-electropolymerize the compounds onto the surface.…”

Section: Cross-linkingmentioning

confidence: 99%

The design, development and application of electrochemical glutamate biosensors

Hughes

Pemberton

Fielden

et al. 2016

TrAC Trends in Analytical Chemistry

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Construction of glutamate biosensor based on covalent immobilization of glutmate oxidase on polypyrrole nanoparticles/polyaniline modified gold electrode

Cited by 66 publications

References 41 publications

Recent progress in nanobiocatalysis for enzyme immobilization and its application

Recent progress in nanobiocatalysis for enzyme immobilization and its application

A Strategy to Employ Polymerised Riboflavin in the Development of Electrochemical Biosensors

The design, development and application of electrochemical glutamate biosensors

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