Construction of integrated linkage map of a recombinant inbred line population of white lupin (<i>Lupinus albus</i> L.)

Abstract

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F8 recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.

Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (&lt;i&gt;Lupinus albus&lt;/i&gt; L.) Represent a Unique Genepool

“…Recently, a newer DArT chip was developed and utilised by Vipin et al [25]; it was assembled primarily from L. albus accessions and showed greater polymorphism but was not available when the Figure 3. Principal coordinate analysis of 94 accessions of Lupinus albus L. based upon combined data from 315 DArT and PCR-based markers.…”

“…Now that an improved DArT array is available [25], there would be merit in extending this analysis to all accessions held in collections world-wide. Nevertheless, we have identified significant genetic diversity among the landraces, varieties and breeding lines-genetic variability that is ready to be explored by breeders and used to donate new or rare alleles to their breeding gene pools.…”

“…These genotypes were important because they included the parents of the mapping population used to produce the L. albus linkage maps [22] [25], to locate the loci for low seed-alkaloid content (pauper) [34], and to develop PCR markers for resistance to anthracnose [35] and phomopsis [31]. In addition, they included parents of other mapping populations made for use in research to identify markers for loci controlling Pleiochaeta Root Rot resistance, and low seed alkaloid content locus exiguus [6].…”

“…DArT analysis was conducted at DArT Pty Ltd (Canberra, Australia, URL: www.diversityarrays.com) using the general procedures previously described [37]. Genomic library construction, array development, hybridisation to microarray, image analysis, and data analysis were performed as described in Vipin et al (2013).…”

Localisation of Loci Involved in Resistance to &lt;i&gt;Diaporthe toxica&lt;/i&gt; and &lt;i&gt;Pleiochaeta setosa&lt;/i&gt; in White Lupin (&lt;i&gt;Lupinus albus&lt;/i&gt; L.)

“…In this study, we utilized the newer, predominantly L. albus DArT array. Details of this array design, platform and genotyping, and scoring of dominant DArT markers have been previously described [29]. Binary data derived from the newer L. albus array was combined with PCR-based markers and DArT data from the multispecies array generated in our previous study [28].…”