Contact lens care solution killing efficacy against Acanthamoeba castellanii by in vitro testing and live-imaging

Kolar, Satya Sree N.; Manarang, Joseph C.; Burns, Alan R.; Miller, William L.; McDermott, Alison M.; Bergmanson, Jan P.G.

doi:10.1016/j.clae.2015.06.006

Cited by 21 publications

(14 citation statements)

References 45 publications

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“…The performance of the comparator solutions against both A . castellanii forms (trophozoites and cysts) was not surprising as similar results have been demonstrated in past studies using a variety of laboratory methods for enumeration and qualitative analysis [ 20 , 25 , 26 ]. Similarly, there have been very few (one) marketed, now discontinued MPS product with any chlorine-based disinfection componentry, and its effectiveness against Acanthamoeba sp.…”

Section: Discussionsupporting

confidence: 83%

“…Comparator solutions failed to kill an appreciable number of Acanthamoeba and suggest that the protozoa could maintain viability for longer periods of time in contact lens holding cases. Prior study of MPS formulations to kill Acanthamoeba have indicated very low or no effectiveness for the commercially available solutions used as comparators in the current study [ 20 , 25 – 29 ]. The inability of current MPS formulations to effectively kill this pathogen may be due to the choice and prevailing concentration of disinfecting agents in the majority of these formulations.…”

Section: Discussionmentioning

confidence: 99%

“…Contact lens storage cases, therefore, represent a major exposure pathway for this pathogen into the eye of contact lens wearers. Current commercially available lens care multipurpose disinfection solutions (MPS) products have shown to provide minimal efficacy against Acanthamoeba species during routine cleaning and while soaking in contact lens cases [ 8 – 20 ]. The overall ineffectiveness of leading commercial MPS formulations against Acanthamoeba is not surprising as the majority of these products contain chemically similar active ingredients which possess minimal antiprotozoal properties.…”

Section: Introductionmentioning

confidence: 99%

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Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii

Fears

Metzinger

Killeen

et al. 2018

J Ophthal Inflamm Infect

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BackgroundA multipurpose contact lens cleaning solution (MPS) containing novel active ingredients under development was compared to two commercially available MPS solutions for effectiveness against Acanthamoeba isolates.MethodsThe Acanthamoeba isolate A. castellanii was propagated for trophozoite or cyst-containing cultures for the purpose of assessment of effectiveness of each MPS. An alamar blue-based cellular respiration assay was used to assess effectiveness against trophozoites; Trypan blue hemocytometer-based microscopic counts measured cysticidal effects. To assess the general antimicrobial potency of each solution as controls for the anti-amoebic assays, comparative bactericidal effectiveness using Serratia marcenses was also performed.ResultsMinimal effectiveness against either Acanthamoeba form was observed from either commercial MPS. In contrast, the novel MPS achieved complete kill within 1 h contact time for both Acanthamoeba trophozoite and cysts. Each commercial MPS required 6 h contact time to achieve a two to three log reduction in S. marcenses. In contrast, the experimental MPS achieved disinfection in 60 min contact time, and complete kill (< 1 CFU) at 90 min.ConclusionsResults suggest that the inclusion of a novel ingredient combination within the MPS under development clearly is required and is ideal for rapid and effective killing of Acanthamoeba species in the context of contact lens disinfection systems. The representative commercially available MPS used in this testing provided minimal effectiveness against the protozoa regardless of contact time. In addition, comparative results with the bacterial agent in the control study show distinct differences in the speed to disinfection with the novel MPS. Future MPS development should consider inclusion of novel chemical entities that are effective against Acanthamoeba species to speed disinfection and further reduce the exposure potential of users of contact lenses and cleaning systems.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii

Fears

Metzinger

Killeen

et al. 2018

J Ophthal Inflamm Infect

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“…However, none of the contact lens disinfectants completely destroyed A. castellanii trophozoites and cyst. These findings are consistent with several recent studies indicating that marketed contact lens disinfectants are ineffective in completely destroying amoebae (Kolar et al, 2015;Padzik et al, 2014;Polat et al, 2007;Ustüntürk and Zeybek, 2014). In particular, there show limited efficacy against cysts.…”

Section: Discussionsupporting

confidence: 92%

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Abjani

Khan

Jung

et al. 2017

Experimental Parasitology

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The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept ® , AO SEPT PLUS, OPTI-FREE ® pure moist ® , Renu ® fresh™, FreshKon ® CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT). The efficacy of contact lens solutions was determined against keratitis-causing microbes, namely: Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, and Acanthamoeba castellanii. In addition, using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, we determined whether combination of both agents can enhance efficacy of marketed contact lens disinfectants against A. castellanii trophozoites and cysts, in vitro. The results revealed that all contact lens disinfectants tested showed potent bactericidal effects exhibiting 100% kill against all bacterial species tested. In contrast, none of the contact lens disinfectants had potent effects against Acanthamoeba cysts viability. When tested against trophozoites, two disinfectants, Oxysept Multipurpose and AO-sept Multipurpose showed partial amoebicidal effects. Using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, the findings revealed that combination of both agents in contact lens disinfectants abolished viability of A. castellanii cysts and trophozoites. Given the inefficacy of contact lens disinfectants tested in this study, these findings present a significant concern to public health. These findings revealed that targeting cyst wall by using cyst wall degrading molecules in contact lens disinfecting solutions will enhance their efficacy against this devastating eye infection.

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“…Unlike hydrogen peroxide systems, which have been reported to have good activity against Acanthamoeba if sufficient time is allowed before neutralization, 7,8 MPS vary in their efficacy against this organism. [9][10][11][12][13] Newer formulations containing dual antimicrobial agents have been introduced for soft lenses which may be more effective, [14][15][16] but further evaluation is needed. With respect to solutions for rigid gas permeable (RGP) lenses, such combination disinfectants are not yet available.…”

mentioning

confidence: 99%

Effects of Lead Phytochemicals of Radix Scutellariae on Acanthamoeba

Cho

Shi

Yap

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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Contact lens care solution killing efficacy against Acanthamoeba castellanii by in vitro testing and live-imaging

Cited by 21 publications

References 45 publications

Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii

Comparative in vitro effectiveness of a novel contact lens multipurpose solution on Acanthamoeba castellanii

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Effects of Lead Phytochemicals of Radix Scutellariae on Acanthamoeba

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