Contactin1a expression is associated with oligodendrocyte differentiation and axonal regeneration in the central nervous system of zebrafish

Schweitzer, Jörn; Gimnopoulos, Dimitrios; Lieberoth, Bettina C; Pogoda, Hans-Martin; Feldner, Julia; Ebert, Anselm; Schachner, Melitta; Becker, Thomas; Becker, Catherina G.

doi:10.1016/j.mcn.2007.02.018

Cited by 42 publications

(58 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[17][18][19][20][21][22][23][24][25][26][27] The lens in zebrafish eyes is spheroid and not ellipsoid as compared with the human eye, and as a consequence, the zebrafish eye has a much lower volume of vitreous compared with the human eye ( Figure 1a). …”

Section: Eye Structurementioning

confidence: 99%

“…28 In contrast to human ganglion cells where the retinal part of the axon is unmyelinated and the axon in the optic nerve is myelinated, in zebrafish the whole axon is myelinated to different extents. 26,27 The photoreceptor outer segment (POS) of zebrafish consists of rods and cones, comparable to the human retina. Anatomically cones are arranged in a mosaic pattern that can be categorized into four types: short single cones (SSCs)-ultraviolet (UV)-sensitive cones, long single cones (LSCs)-blue-sensitive cones, and double cones (DCs) consisting of short (green sensitive) and long (red sensitive) cones.…”

Section: Eye Structurementioning

confidence: 99%

See 1 more Smart Citation

Zebrafish—on the move towards ophthalmological research

Chhetri

Jacobson

Gueven

2014

Eye

138

114

View full text Add to dashboard Cite

Millions of people are affected by visual impairment and blindness globally, and the prevalence of vision loss is likely to increase as we are living longer. However, many ocular diseases remain poorly controlled due to lack of proper understanding of the pathogenesis and the corresponding lack of effective therapies. Consequently, there is a major need for animal models that closely mirror the human eye pathology and at the same time allow higher-throughput drug screening approaches. In this context, zebrafish as an animal model organism not only address these needs but can in many respects reflect the human situation better than the current rodent models. Over the past decade, zebrafish have become an established model to study a variety of human diseases and are more recently becoming a valuable tool for the study of human ophthalmological disorders. Many human ocular diseases such as cataract, glaucoma, diabetic retinopathy, and age-related macular degeneration have already been modelled in zebrafish. In addition, zebrafish have become an attractive model for pre-clinical drug toxicity testing and are now increasingly used by scientists worldwide for the discovery of novel treatment approaches. This review presents the advantages and uses of zebrafish for ophthalmological research.

show abstract

Section: Eye Structurementioning

confidence: 99%

Section: Eye Structurementioning

confidence: 99%

Zebrafish—on the move towards ophthalmological research

Chhetri

Jacobson

Gueven

2014

Eye

138

114

View full text Add to dashboard Cite

show abstract

“…The Zwilling-A and -B proteins are also expressed in both CNS and PNS and have been hypothesized to play roles in the adhesion of myelin lamellae in the zebrafish nervous system (Schaefer and Br€ osamle, 2009). Contactin1a (Cntn1/F3/F11), which plays a role in glial cell differentiation and myelination, is expressed in CNS and PNS of developing zebrafish, but in the adult becomes restricted to the optic axon layer in the retina, which contains only noncompact ''loose'' myelin (Schweitzer et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Claudin k is specifically expressed in cells that form myelin during development of the nervous system and regeneration of the optic nerve in adult zebrafish

Münzel

Schaefer

Obirei

et al. 2011

Glia

Self Cite

View full text Add to dashboard Cite

The zebrafish has become an important model organism to study myelination during development and after a lesion of the adult central nervous system (CNS). Here, we identify Claudin k as a myelin-associated protein in zebrafish and determine its localization during development and adult optic nerve regeneration. We find Claudin k in subcellular compartments consistent with location in autotypic tight junctions of oligodendrocytes and myelinating Schwann cells. Expression starts in the hindbrain at 2 days (mRNA) and 3 days (protein) postfertilization and is maintained in adults. A newly generated claudin k:green fluorescent protein (GFP) reporter line allowed us to characterize oligodendrocytes in the adult retina that express Claudin k and olig2, but not P0 and uniquely only form loose wraps of membrane around axons. After a crush of the adult optic nerve, Claudin k protein levels were first reduced and then recovered within 4 weeks postlesion, concomitant with optic nerve myelin de- and regeneration. During optic nerve regeneration, oligodendrocytes, many of which were newly generated, repopulated the lesion site and exhibited increasing morphological complexity over time. Thus, Claudin k is a novel myelin-associated protein expressed by oligodendrocytes and Schwann cells from early stages of wrapping and myelin formation in zebrafish development and adult regeneration, suggesting important functions of the gene for myelin formation and maintenance. Our Claudin k antibodies and claudin k:GFP reporter line represent excellent ways to visualize oligodendrocyte and Schwann cell differentiation in vivo.

show abstract

“…Myelin protein zero (P0), 4 expressed in Schwann cells in mammals, shows axonal growthpromoting properties in vitro (9), and the mpz gene encoding the zebrafish P0 orthologue is strongly up-regulated in oligodendrocytes in regenerating white matter tracts following CNS axonal injury (10). Similarly, contactin1a, implicated in axonal growth and myelination, is up-regulated in zebrafish optic nerve oligodendrocytes following crush injury (11).…”

mentioning

confidence: 99%

Different Mechanisms Regulate Expression of Zebrafish Myelin Protein Zero (P0) in Myelinating Oligodendrocytes and Its Induction following Axonal Injury

Bai

Parris

Burton

2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Zebrafish CNS oligodendrocytes up-regulate axon growth-promoting genes following axonal injury. Results: Separate cis-regulatory elements mediate mpz expression in myelinating oligodendrocytes and its transcriptional induction by axonal damage. Conclusion: Different mechanisms specify oligodendroglial mpz expression in the intact and injured CNS. Significance: Mechanisms underlying the zebrafish repair response might be exploited to enhance axonal regeneration in the mammalian CNS.

show abstract

Contactin1a expression is associated with oligodendrocyte differentiation and axonal regeneration in the central nervous system of zebrafish

Cited by 42 publications

References 71 publications

Zebrafish—on the move towards ophthalmological research

Zebrafish—on the move towards ophthalmological research

Claudin k is specifically expressed in cells that form myelin during development of the nervous system and regeneration of the optic nerve in adult zebrafish

Different Mechanisms Regulate Expression of Zebrafish Myelin Protein Zero (P0) in Myelinating Oligodendrocytes and Its Induction following Axonal Injury

Contact Info

Product

Resources

About