This paper examines the role of digital twins (DTs) in promoting sustainability within seaport operations and logistics. DTs have emerged as promising tools for enhancing seaport performance. Despite the recognized potential of DTs in seaports, there is a paucity of research on their practical implementation and impact on seaport sustainability. Through a systematic literature review, this study seeks to elucidate how DTs contribute to the sustainability of seaports and to identify future research and practical applications. We reviewed and categorized 68 conceptual and practical digital applications into ten core areas that effectively support economic, social, and environmental objectives in seaports. Furthermore, this paper proposes five preliminary potential applications for DTs where practical implementations are currently lacking. The primary findings indicate that DTs can enhance seaport sustainability by facilitating real-time monitoring and decision-making, improving safety and security, optimizing resource utilization, enhancing collaboration and communication, and supporting the development of the seaport ecosystem. Additionally, this study addresses the challenges associated with DT implementation, including high costs, conflicting stakeholder priorities, data quality and availability, and model validation. The paper concludes with a discussion of the implications for seaport managers and policymakers.