Contaminated air conditioners as potential source for contaminating operation theater environment

Kelkar, Uday; Kulkarni, Sandhya Sadanand

doi:10.3396/ijic.v8i1.009.12

Cited by 4 publications

(6 citation statements)

References 3 publications

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“…However, fungus in air conditioners in tutorial rooms of Faculty of Medicine shows a higher prevalence of Aspergillus and Mucor. 11 Nonetheless, there are no Standards or Threshold Limit Values (TLVs) established yet to judge what the acceptable, tolerable, or normal quantity of mold. 13,14 In conclusion, it is revealed that the pathogenic fungi are presence in the air conditioners from tutorial rooms with the frequency of 94.1%.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Pathogenic Fungi from Air Conditioners in Tutorial Rooms of the Faculty of Medicine, Universitas Padjadjaran

Govindasamy¹,

Husin²,

Syukriani³

et al. 2014

amj

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Background: Awareness about health problems caused by air conditioner is very important. Thus, it is crucial to have knowledge about proper maintenance of air conditioner. At the Faculty of Medicine, Universitas Padjadjaran, air conditioners are used in every tutorial rooms. This study was performed to provide adequate information on the fungi, such as Aspergillus, Penicillium and Mucor, found in air conditioners. Methods: A descriptive laboratory study was used to identify the presence and the type of pathogenic fungi from air conditioners in tutorial rooms. Thirty-four samples were collected from the air outlet grille of the air conditioners and cultured on Sabouraud agar at 27°C for 2 weeks. Fungi presence were then identified microscopically Results: The results showed that the majority of air conditioners in tutorial rooms of the Faculty of Medicine, University Padjadjaran contained many types of fungus that grew in Sabouraud agar. From 34 samples, thirty two samples were positive and 2 samples were negative. Various fungus have been identified, those were Penicillium (37.5%), Aspergillus (25%), Mucor (2.5%) and unidentified (35%) Conclusions: The majority of air conditioners in tutorial rooms of the Faculty of Medicine, Universitas Padjadjaran contained many types of opportunistic fungus.

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Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Pathogenic Fungi from Air Conditioners in Tutorial Rooms of the Faculty of Medicine, Universitas Padjadjaran

Govindasamy¹,

Husin²,

Syukriani³

et al. 2014

amj

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“…The Samples were collected in four seasons of the year. About 38 samples each season from indoor air and 11 samples from air conditioners collected by using Settle Plate Method (Passive Air Sampling) on blood agar for bacteria culture (Genet et al, 2011;Kelkar and Kulkarni, 2011) and Sabouraud's Dextrose Agar (SDA) plate plus 50µg/ml of gentamicin for fungi culture (Kelkar and Kulkarni, 2011).…”

Section: Methodsmentioning

confidence: 99%

“…Each plate was leaving open to the air for a 30 minutes (Kelkar and Kulkarni, 2011), a meter to 1.5m above the floor and a meter from the wall (Ekhaise and Ogboghodo, 2011). The air samples collected (at 9-11 AM) within a given day.…”

Section: Methodsmentioning

confidence: 99%

“…The air samples collected (at 9-11 AM) within a given day. The blood agars settle plate then incubate at 35-37° C for 24-48 hours (Ekhaise and Ogboghodo, 2011) while the fungal culture plates are incubate at room temperature (22°C -26 C) for 3 -7 days (Ekhaise et al, 2010;Garcia-Cruz et al, 2012;Kelkar and Kulkarni, 2011;Bhaita and Viskwakarma, 2010).…”

Section: Methodsmentioning

confidence: 99%

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Microbial Air Quality in Medical Laboratory Rooms at Benghazi Center of Infectious Diseases and Immunity

Buzayan¹,

El-Garbulli²

2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoThe control of indoor air quality plays an important role in the prevention of cross infection in hospitals to protect both hospital staff and patients. This study was carried out to determine the variation in microbiota of medical laboratory at Benghazi center infectious diseases and immunity. Samples were collected by using the settled plate techniques for the enumeration of bacterial and fungal isolates. The air specimens were collected through four seasons in the morning between the hours of 9 am and 11 am from air indoor medical lab and swab samples collected from the filters of the air conditioning units in autumn, spring and summer seasons. Indoor air gram positive bacteria, accounting (99.72%) was significantly higher than that of gram negative bacteria (0.28%). The most common bacteria genus found in all seasons were Staphylococcus (46.62%) Micrococcus (27.31%), followed by Kocuria (13.42% ). The most abundant isolated bacteria in the three seasons was Bacillus circulans while Penicillium sp. was the most abundant fungi in air conditioning isolates. In indoor air samples of medical laboratory rooms showed contamination with bacteria and fungi under the acceptable levels, when compared with cited in this study. Indoor airborne bacteria and fungi concentration were depended on place of isolation and seasons.

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“…Other opportunistic fungi isolated in this study are also known to cause nosocomial infections. 6,7,27 The spores of Aspergillus have a diameter of 1.5-3.5 µm and a settling velocity of approximately 1m/hour in still air. With this buoyancy, the spores which resist drying can remain in the air indefinitely 7 and get settled into surgical wounds as well as inhaled.…”

Section: Discussionmentioning

confidence: 99%

Is fumigation enough for air conditioning units in operation theatres and Intensive care units?

et al. 2016

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Contaminated air conditioners as potential source for contaminating operation theater environment

Cited by 4 publications

References 3 publications

Isolation and Identification of Pathogenic Fungi from Air Conditioners in Tutorial Rooms of the Faculty of Medicine, Universitas Padjadjaran

Isolation and Identification of Pathogenic Fungi from Air Conditioners in Tutorial Rooms of the Faculty of Medicine, Universitas Padjadjaran

Microbial Air Quality in Medical Laboratory Rooms at Benghazi Center of Infectious Diseases and Immunity

Is fumigation enough for air conditioning units in operation theatres and Intensive care units?

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