“…In the present study, the spatial evaluation was carried out in three sampling sites located at the lower estuary of the SES (S1, S2, and S3) (Fig. 1), where there is a marked pollution gradient demonstrated by studies assessing the increased concentrations of contaminants towards upper estuary (from S3 to S1) (Buruaem et al, 2013;Harayashiki et al, 2022;Oliveira et al, 2020). Indeed, high levels have been reported in S1 for PAHs, organochlorine compounds (Begliomini et al, 2017), estrogens (Pusceddu et al, 2019), antifouling biocides (Abreu et al, 2020), toxic metals (Kim et al, 2017) and marine litter (Ribeiro et al, 2021a).…”