Content and Composition of the Mucoprotein Fraction of Human Serum (Seromucoid) in Disease, With Special Reference to Hematologic Disorders1

Electrophoretic Studies of the Vitamin B12-Binding Protein of Normal and Chronic Myelogenous Leukemia Serum*

“…These chemical similarities between the normal and the CML B12BP strongly suggest that the increased concentrations of vitamin B12 uniformly found in CML serum in relapse is due to an increase in concentration of the normal B12BP rather than to an abnormal protein. Despite the tremendous increases in concentration of the B12BP in CML sera (amounting to as much as 100-fold in one patient we have studied), only slight increases in the concentration of the total seromucoid fraction have been reported in this disease (24). However, the concentration of B12BP in normal serum is probably minute as compared to that of the total seromucoid.…”

The Plasma Vitamin B12 Binding Substance: I. Its Detection in the Seromucoid Fraction of Plasma From Normal Subjects and Patients With Chronic Myelo-Cytic Leukemia *

“…Total seromucoid is also known to rise in a variety of diseases associated with traumatic, inflammatory or degenerative tissue changes. These findings have been well reviewed by Winzler (23), Greenspan (25), and Moschides, Stefanini, Magalini and Kistner (26). In addition, however, there is evidence that the carbohydrate to protein ratio of seromucoid may vary in different diseases (27,28) and also that the relationship between carbohydrate components themselves may vary (29).…”

“…In addition, however, there is evidence that the carbohydrate to protein ratio of seromucoid may vary in different diseases (27,28) and also that the relationship between carbohydrate components themselves may vary (29). It has been previously suggested (26) that, since seromucoid is a heterogeneous material, these changes could result from the alteration in a specific component of this fraction. The present data suggest that such is the case in plasma of subjects with CML, in which the B12 binding protein is increased.…”

Co60 Vitamin B12 Binding Capacity of Serum in Persons with Hematologic Disorders, Various Medical Diseases and Neoplasms

“…Recently, the seromucoid which has an electrophoretic mobility similar to that of alpha 1 and alpha 2 globulins has been found to include the specific binding ( 19,30). The total seromucoids are increased in human subject5 with neoplastic disease, trauma, inflammation, degenerative tissue changes, and in certain tumor bearing animals, (1, 8,22,31). However, the data in the present study do not disclose any relationship of vitamin B i g serum binding capacity to diseases other than most cases of chronic myelocytic leukemia, about half of the patients with polycythemia Vera, myeloid metaplasia with antecedent histories of polycythemia or chronic myelocytic leukemia, and some cases of acute myeloblastic and lymphoblastic leukemia.…”