Continuous enzyme aqueous two-phase extraction using a novel tubular mixer-settler in multi-step counter-current arrangement

Vázquez-Villegas, Patricia; Aguilar, Oscar; Rito‐Palomares, Marco

doi:10.1016/j.seppur.2014.12.005

Cited by 28 publications

(19 citation statements)

References 24 publications

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“…This recovery platform usually requires the sample load to represent a minimal weight fraction of the system, compared to the ATPS components (in the production of B‐phycoerythrin, the system components were 60% w/w, while the sample represented the 40% w/w). This is one of the main drawbacks when using ATPS, but has been addressed in other researches in which optimization strategies coupled with appropriate experimental design may aid in the selection of an optimal sample selection to be loaded into the desired ATPS . To further reduce costs, the recycling of components can be considered.…”

Section: Resultsmentioning

confidence: 99%

“…This is one of the main drawbacks when using ATPS, but has been addressed in other researches in which optimization strategies coupled with appropriate experimental design may aid in the selection of an optimal sample selection to be loaded into the desired ATPS. 28 To further reduce costs, the recycling of components can be considered. Several studies using ATPS-based technologies have developed this approach 14,29,30 concluding with an economic analysis that demonstrates that the use of additional operations to recover ATPS components does not increase the cost of the final product.…”

Section: Deterministic Analysismentioning

confidence: 99%

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Economic analysis of pilot‐scale production of B‐phycoerythrin

Torres‐Acosta

Ruiz‐Ruiz

Aguilar‐Yáñez

et al. 2016

Biotechnology Progress

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β-Phycoerythrin is a color protein with several applications, from food coloring to molecular labeling. Depending on the application, different purity is required, affecting production cost and price. Different production and purification strategies for B-phycoerythrin have been developed, the most studied are based on the production using Porphyridium cruentum and purified using chromatographic techniques or aqueous two-phase systems. The use of the latter can result in a less expensive and intensive recovery of the protein, but there is lack of a proper economic analysis to study the effect of using aqueous two-phase systems in a scaled-up process. This study analyzed the production of B-Phycoerythrin using real data obtained during the scale-up of a bioprocess using specialized software (BioSolve, Biopharm Services, UK). First, a sensitivity analysis was performed to identify critical parameters for the production cost, then a Monte Carlo analysis to emulate real processes by adding uncertainty to the identified parameters. Next, the bioprocess was analyzed to determine its financial attractiveness and possible optimization strategies were tested and discussed. Results show that aqueous two-phase systems retain their advantages of low cost and intensive recovery (54.56%); the costs of production per gram calculated (before titer optimization: US$15,709 and after optimization: US$2,374) allowed to obtain profit (in the range of US$millions in a 10-year period) for a potential company taking this production method by comparing the production cost against commercial prices. The bioprocess analyzed is a promising and profitable method for the generation of a highly purified B-phycoerythrin. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1472-1479, 2016.

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Section: Resultsmentioning

confidence: 99%

Section: Deterministic Analysismentioning

confidence: 99%

Economic analysis of pilot‐scale production of B‐phycoerythrin

Torres‐Acosta

Ruiz‐Ruiz

Aguilar‐Yáñez

et al. 2016

Biotechnology Progress

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“…The outlet flow was continuously separated as coacervate phase and diluted phase by an in line separator. Two configurations of separators were used to observe if the extractive phase could be recovered with minimal cross‐contamination of the phases . All fermentations were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

Chávez-Castilla

Aguilar

2016

J. Chem. Technol. Biotechnol.

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BACKGROUND In situ product recovery (ISPR), or extractive fermentation, is a strategy for the primary recovery of metabolites. A micellar aqueous two‐phase system (ATPS) composed of a surfactant‐rich phase was coupled to batch and continuous fermentations for the production and in situ recovery of the red alkaloid prodigiosin from Serratia marcescens. A cloud point ATPS was formed at 24 °C using triton X‐114 and used as a production/extraction system for this metabolite, which shows therapeutic properties as antibiotic, anti‐proliferative and anti‐malarial activities. RESULTS The integration of the micellar ATPS in batch fermentation resulted in the production of 342 mg prodigiosin L−1 with a recovery of 81% in the extractive phase. When a continuous fermentation was performed, based on a cloud point system, 1.2‐fold prodigiosin content was obtained compared with batch mode and a recovery of 88% of the metabolite. This continuous operation resulted in 0.43 g of purified prodigiosin from a 1.5 L bioreactor with a concentration of 872 mg prodigiosin L−1 of extractive phase and a purity of 82%, demonstrating particular selectivity of the system for prodigiosin as a primary recovery method. This is the first report on the continuous production of this generally recognized secondary metabolite. CONCLUSION The experimental approach proposed proved that an ISPR system could lead to the establishment of a more efficient process for continuous large‐scale prodigiosin production. The proposal of a primary recovery strategy with only one bioprocess step enhances this metabolite production by lowering the inhibiting properties of prodigiosin for the microorganism. © 2016 Society of Chemical Industry

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“…[35][36][37] The selected invertase titer (151.5 U mL −1 ) was an average of that reported in the two previous studies. [12][13][14][15] Values of invertase activity recovery yield are generally observed to be above 100% 8,[10][11][12][13][14] which may be caused by the removal of natural inhibitors or a better chemical environment for enzyme activity.…”

Section: Comparison Between Atps and Tpp Model Constructionmentioning

confidence: 99%

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

Torres‐Acosta

Morales‐Guzman

Ruiz‐Ruiz

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Invertase use in the food industry is limited by production costs. Alternative strategies for extraction, such as aqueous two‐phase systems (ATPS) and three‐phase partitioning (TPP), could be economically feasible for yeast invertase. Economic modeling of bioprocesses makes possible the identification of critical parameters for production costs and emulation of real scenarios, moreover incorporation of uncertainty is possible. This study performed an economic analysis on the production of invertase using ATPS or TPP, also a virtual optimization of ATPS was done. RESULTS TPP provided a lower production cost than ATPS ($145 vs $59.3 per 1 million enzymatic units, respectively). The critical parameter for TPP is recovery yield as it is highly dependent on operating conditions. In contrast, ATPS is dependent on materials costs as the sample load is smaller for ATPS, requiring a larger system. Although TPP provided a lower cost, t‐butanol hinders its acceptance. CONCLUSION Virtual optimization of ATPS found that varying sample load or system size is not enough to have a lower production cost than TPP, but provided insights for reduction of production costs and development of a safer technique. This study provides a framework for the virtual analysis of ATPS and TPP to evaluate their processes and reduce production costs. © 2018 Society of Chemical Industry

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Continuous enzyme aqueous two-phase extraction using a novel tubular mixer-settler in multi-step counter-current arrangement

Cited by 28 publications

References 24 publications

Economic analysis of pilot‐scale production of B‐phycoerythrin

Economic analysis of pilot‐scale production of B‐phycoerythrin

An integrated process for thein siturecovery of prodigiosin using micellar ATPS from a culture ofSerratia marcescens

Monte Carlo economic analysis of Baker's yeast invertase purification using two‐ and three‐phase partitioning

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