Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenes

Tanisho, Shigeharu; Ishiwata, Yoshihisa

doi:10.1016/0360-3199(94)90197-x

Cited by 121 publications

(39 citation statements)

References 4 publications

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“…Here, H 2 yields varied from 0.6 to 3.8 mol/mol sugar [111,127,167] and iii) Bacillus coagulans, B. licheniformis and B. subtilis have been shown to evolve 1.5 to 2.36 mol H 2 /mol glucose [69,84,91]. B. licheniformis and B. subtilis could also generate H 2 from damaged wheat grains at the rate of 45 to 64 L/ kg Total solids [64,158].…”

Section: In Search Of Potential ''Wonder'' Bug(s) For Hydrogen Producmentioning

confidence: 99%

Microbial diversity and genomics in aid of bioenergy

Kalia

Purohit

2008

J Ind Microbiol Biotechnol

106

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In view of the realization that fossil fuels reserves are limited, various options of generating energy are being explored. Biological methods for producing fuels such as ethanol, diesel, hydrogen (H2), methane, etc. have the potential to provide a sustainable energy system for the society. Biological H2 production appears to be the most promising as it is non-polluting and can be produced from water and biological wastes. The major limiting factors are low yields, lack of industrially robust organisms, and high cost of feed. Actually, H2 yields are lower than theoretically possible yields of 4 mol/mol of glucose because of the associated fermentation products such as lactic acid, propionic acid and ethanol. The efficiency of energy production can be improved by screening microbial diversity and easily fermentable feed materials. Biowastes can serve as feed for H2 production through a set of microbial consortia: (1) hydrolytic bacteria, (2) H2 producers (dark fermentative and photosynthetic). The efficiency of the bioconversion process may be enhanced further by the production of value added chemicals such as polydroxyalkanoate and anaerobic digestion. Discovery of enormous microbial diversity and sequencing of a wide range of organisms may enable us to realize genetic variability, identify organisms with natural ability to acquire and transmit genes. Such organisms can be exploited through genome shuffling for transgenic expression and efficient generation of clean fuel and other diverse biotechnological applications.

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Section: In Search Of Potential ''Wonder'' Bug(s) For Hydrogen Producmentioning

confidence: 99%

Microbial diversity and genomics in aid of bioenergy

Kalia

Purohit

2008

J Ind Microbiol Biotechnol

106

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“…Hydrogen yields were improved to 1.4 mg of H2/g of DS using the boiled sludge and to 1.5∼2.1 mmol of H2/g of COD with sludge pretreated by sterilization or freezing-and-thawing (11). However, pure culture method was used in most studies for biohydrogen production (11)(12)(13)(14)(15) and thus made the biohydrogen production process more complicated because of necessary sterilization. Therefore, microflora enriched from a natural population of bacteria by various methods such as heatshocking and acid-base treatment are currently used in biohydrogen production studies (2,(5)(6)(7)17).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biohydrogen Production from Sewage Sludge with Alkaline Pretreatment

Mulin

Liu

Wei

2004

Environ. Sci. Technol.

379

159

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Batch tests were carried out to analyze influences of the alkaline pretreatment and initial pH value on biohydrogen production from sewage sludge. Experimental results of the impact of different initial pH on biohydrogen production showed that both the maximal hydrogen yield occurred and that no methane was detected in the tests of at the initial pH of 11.0. The final pH decreased at the initial pH of 7.0-12.5 but increased at the initial pH of 3.0-6.0, probably due to the combination of solubilized protein from sludge and the formation of volatile fatty acids (VFAs) and ammonia during biohydrogen fermentation. The performance of biohydrogen production using the raw sludge and the alkaline pretreated sludge was then compared in batch fermentation tests at the initial pH of 11.0. The hydrogen yield was increased from 9.1 mL of H 2 /g of dry solids (DS) of the raw sludge to 16.6 mL of H 2 /g of DS of the alkaline pretreated sludge. No methane and less carbon dioxide (0.8% of control) were present in the biohydrogen production from the alkaline pretreated sludge. These results clearly showed that biohydrogen production could be enhanced and maintained stable by the combination of the high initial pH and alkaline pretreatment. The mechanism of biohydrogen production from sewage sludge at high initial pH was therefore investigated because the results of this study were different from previous studies of biohydrogen production. Results showed that protein was the major substrate for biohydrogen production from sewage sludge and that Eubacterium multiforme and Paenibacillus polymyxa were the dominant bacteria in biohydrogen production from alkaline pretreated sludge at an initial pH of 11.0. The combination of alkaline pretreatment and high initial pH could not only maintain a suitable pH range for the growth of dominant hydrogen-producing anaerobes but also inhibit the growth of hydrogen-consuming anaerobes. In addition, the changes in pH value, oxidationreduction potential, VFAs and soluble COD during hydrogen fermentation were also discussed.

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“…Of these, Enterobacteriaceae were characterized as a group of strong and efficient producers (Tanisho et al 1987). Most members of this family have several advantages for hydrogen production, such as rapid growth rate, the ability to utilize a wide range of carbon sources, and low sensitivity to dissolved oxygen, H 2 pressure, and pH (Nakashimada et al 2002;Kumar and Das 2000;Tanisho and Ishiwata 1994). Enterobacter aerogenes strains, such as HU-101, E-82005, and HO-39, have been widely studied in the context of the evolution of hydrogen production (Yokoi et al 1995;Rachman et al 1997;Tanisho et al 1987).…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production

Zhao

Cheng

et al. 2009

World J Microbiol Biotechnol

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The hydrogenase gene from Enterobacter cloacae (IIT-BT 08) was amplified and inserted into a prokaryotic expression vector to create a recombinant plasmid (pGEX-4T-2-Cat/hydA). The recombinant plasmid was transformed into a hydrogen-producing strain of Enterobacter aerogenes (ATCC13408). SDS-PAGE and western blot analysis confirmed the successful expression of the GST-tagged hydA protein. Anaerobic fermentation for the production of hydrogen from glucose was investigated using E. aerogenes ATCC13408 and the recombinant strain. The results showed that the hydrogen yield markedly increased, from 442.82 ± 22.61 ml/g glucose in the ATCC13408 strain to 864.02 ± 36.8 ml/g glucose in the recombinant. The maximum rate of hydrogen production was found to be 53.49 ± 3.34 ml l -1 h -1 using 1% (w/v) glucose as the substrate at pH 6.0 and a reaction temperature of 37°C.

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Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenes

Cited by 121 publications

References 4 publications

Microbial diversity and genomics in aid of bioenergy

Microbial diversity and genomics in aid of bioenergy

Enhanced Biohydrogen Production from Sewage Sludge with Alkaline Pretreatment

Heterologous expression of a hydrogenase gene in Enterobacter aerogenes to enhance hydrogen gas production

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