Continuous production of pectic oligosaccharides from onion skins with an enzyme membrane reactor

Baldassarre, Stefania; Babbar, Neha; Roy, Sandra; Dejonghe, Winnie; Maesen, Miranda; Sforza, Stefano; Elst, Kathy

doi:10.1016/j.foodchem.2017.10.055

Cited by 45 publications

(11 citation statements)

References 22 publications

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“…Other authors reported the use of Viscozyme to obtain POS from different origins, either directly from pectin-rich byproducts, such as lemon peel, orange peel, and sugar beet pulp, 3,4,34 or pectin isolated from sugar beet pulp, onion skins, rapeseed cake, and PGA. 5,6,30,35 Our results agree with these studies where high amounts of low M w POS were obtained. On the other hand, Glucanex has been employed in the digestion of protoplasts from fungal spores 36 and to obtain POS and modified pectin from artichoke pectin.…”

Section: Journal Of Agricultural and Food Chemistrysupporting

confidence: 92%

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Enzymatic Production and Characterization of Pectic Oligosaccharides Derived from Citrus and Apple Pectins: A GC-MS Study Using Random Forests and Association Rule Learning

Sabater

Ferreira-Lazarte

Montilla

et al. 2019

J. Agric. Food Chem.

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Pectic oligosaccharides (POS) from citrus and apple pectin hydrolysis using ViscozymeL and Glucanex200G have been obtained. According to the results, maximum POS formation was achieved from citrus pectin after 30 min of hydrolysis with ViscozymeL, with a yield of 652 mg g −1 and average molecular mass (M w ) of 0.8−2.5 kDa, while with Glucanex200G, the yield was 518 mg g −1 and M w was 0.8−7.1 kDa. Digalacturonic and trigalacturonic acids were identified among other low M w compounds as di-and tri-POS. In addition, differences in GC-MS spectra of all oligosaccharides found in the hydrolysates were studied by employing random forests and other algorithms to identify structural differences between the obtained POS, and high prediction rates were shown for new samples. Chemical structures were proposed for some influential m/z ions, and 12 association rules that explain differences according to pectin and enzyme origin were built. This information could be used to establish structure−function relationships of POS.

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Section: Journal Of Agricultural and Food Chemistrysupporting

confidence: 92%

“…POS can be obtained from depolymerization of raw materials or purified pectins mainly extracted from apple, citrus peel, and sugar beet byproducts, using enzymes, chemical reagents (acids), or combined methods. , Also, other substrates, such as onion skins, rapeseed cake, and artichoke pectin, have been used to obtain POS.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Production and Characterization of Pectic Oligosaccharides Derived from Citrus and Apple Pectins: A GC-MS Study Using Random Forests and Association Rule Learning

Sabater

Ferreira-Lazarte

Montilla

et al. 2019

J. Agric. Food Chem.

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“…POS are obtained by the depolymerization of pectin, a plant complex macromolecule made up of several monosaccharides [101]. Pectins are most importantly composed of a linear backbone of α-(1→4)-linked D-Galacturonic acid (GalA) units that can be partially acetylated and/or methylated [102].…”

Section: Non-digestible Oligosaccharidesmentioning

confidence: 99%

Non-Digestible Oligosaccharides and Short Chain Fatty Acids as Therapeutic Targets against Enterotoxin-Producing Bacteria and Their Toxins

Asadpoor

Ithakisiou

Henricks

et al. 2021

Toxins

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Enterotoxin-producing bacteria (EPB) have developed multiple mechanisms to disrupt gut homeostasis, and provoke various pathologies. A major part of bacterial cytotoxicity is attributed to the secretion of virulence factors, including enterotoxins. Depending on their structure and mode of action, enterotoxins intrude the intestinal epithelium causing long-term consequences such as hemorrhagic colitis. Multiple non-digestible oligosaccharides (NDOs), and short chain fatty acids (SCFA), as their metabolites produced by the gut microbiota, interact with enteropathogens and their toxins, which may result in the inhibition of the bacterial pathogenicity. NDOs characterized by diverse structural characteristics, block the pathogenicity of EPB either directly, by inhibiting bacterial adherence and growth, or biofilm formation or indirectly, by promoting gut microbiota. Apart from these abilities, NDOs and SCFA can interact with enterotoxins and reduce their cytotoxicity. These anti-virulent effects mostly rely on their ability to mimic the structure of toxin receptors and thus inhibiting toxin adherence to host cells. This review focuses on the strategies of EPB and related enterotoxins to impair host cell immunity, discusses the anti-pathogenic properties of NDOs and SCFA on EPB functions and provides insight into the potential use of NDOs and SCFA as effective agents to fight against enterotoxins.

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“…filtration of fruit juice or olive mill waste water) and pectin causes membrane fouling (Gebreyohannes et al, 2013). In the work carried out by Baldassarre et al (2018), a discon tinuous (used as pre-treatment) and a continuous membrane reactor with free enzyme were used. This permitted to increase the volumetric productivity up to five times, demonstrating a real advantage respect to the traditional batch reactor.…”

Section: Pectirzase and Mer In Biorefineriesmentioning

confidence: 99%

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

Mazzei

Gebreyohannes

Papaioannou

et al. 2021

Bioresource Technology

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Continuous production of pectic oligosaccharides from onion skins with an enzyme membrane reactor

Cited by 45 publications

References 22 publications

Enzymatic Production and Characterization of Pectic Oligosaccharides Derived from Citrus and Apple Pectins: A GC-MS Study Using Random Forests and Association Rule Learning

Enzymatic Production and Characterization of Pectic Oligosaccharides Derived from Citrus and Apple Pectins: A GC-MS Study Using Random Forests and Association Rule Learning

Non-Digestible Oligosaccharides and Short Chain Fatty Acids as Therapeutic Targets against Enterotoxin-Producing Bacteria and Their Toxins

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

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