Continuous (R)-mandelic acid production in an enzyme membrane reactor

Kinetic and modelling studies of NAD+ and poly(ethylene glycol)-bound NAD+ in horse liver alcohol dehydrogenase

“…With isopropanol kca t even drops by a factor of four. With most dehydrogenases similar results have been found [2][3][4][5]37]. Only in a few cases comparable activities for NAD + and PEG-NAD + have been reported [3,41. The K M value for PEG-NAD ÷ is significantly larger than the K M for NAD ÷, which has also been observed with other dehydrogenases [2,5,39].…”

Continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor

“…The strategy and importance of modeling in finding suitable operation conditions especially when dealing with multicomponent enzyme reaction systems has been demonstrated previously (Vasic-Racki et al, 1989;Wichmann et al, 1981). The complete rate equations representing the kinetic model for the continuous enzymatic process investigated in this study (cf.…”

“…The aim was to establish a very simple, but not mechanistic, kinetic model capable of describing the experimental conversion data with acceptable accuracy (Vasic-Racki et al, 1989). It was found that Eqs.…”

“…Fig. 1) were derived from the mass balance of an ideal, continuously stirred tank reactor operated at outlet conditions, from the mass balance of the coupled enzymatic reactions (Vasic-Racki et al, 1989) and the enzyme kinetic equations taking into account the previously determined rate constants and retention coefficients of the coenzyme. The mathematical model consisted of rate equations (2)- (7) …”

Mandelate Racemase and Mandelate Dehydrogenase Coexpressed RecombinantEscherichia coliin the Synthesis of Benzoylformate

“…5) It is also biologically prepared by enzyme catalysis and microbial transformation, including mandelate dehydrogenase, [6][7][8][9][10][11] phenylglycine aminotransferase, 12) Saccharomycopsis lipolytica, 13) Candida lipolytica, 14,15) Rhodococcus sp., 1) Corynebacterium, 16,17) Brevibacterium, 17,18) Amycolatopsis, 17) Streptomyces, 17,19) Pseudomonas, 17,20,21) and Alcaligenes. 17,22) Biocatalysts are powerful tools used in the industrial production of valuable organic chemicals under mild conditions.…”