2008
DOI: 10.1039/b710303d
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Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies

Abstract: We present a particle-sorting device based on the opposition of dielectrophoretic forces. The forces are generated by an array of electrode chambers located in both sidewalls of a main flow channel. Particles with different dielectric response perceive different force magnitudes and are therefore continuously focused to different streamlines in the flow channel. We relate the particles' dielectric response to their output position in the downstream channel. We demonstrate the performance of the device by separ… Show more

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Cited by 124 publications
(88 citation statements)
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“…In addition to validating cell synchronization, these experiments also show yeast viability, since in general all the cells selected by the sorting procedure (N z 30 for convenient observation in an outlet channel) divide. For the medium conductivity used in the experiments described above, dead cells lack a cross-over in the frequency range considered, 20 thus selecting the cells with the most negative opacity value also eliminates the dead cells.…”
Section: Synchronizationmentioning
confidence: 99%
See 1 more Smart Citation
“…In addition to validating cell synchronization, these experiments also show yeast viability, since in general all the cells selected by the sorting procedure (N z 30 for convenient observation in an outlet channel) divide. For the medium conductivity used in the experiments described above, dead cells lack a cross-over in the frequency range considered, 20 thus selecting the cells with the most negative opacity value also eliminates the dead cells.…”
Section: Synchronizationmentioning
confidence: 99%
“…This opposition of dielectrophoretic forces eliminates the first order dependence of the force on the volume, 19 increasing the sensitivity to changes in cell shape throughout the division cycle. The arrangement has two additional important advantages: 18,20 First, the cell sorting takes place perpendicular to the flow, such that the device can be operated continuously; and second, the cells are pushed towards a characteristic equilibrium position for the different division stages, independently of the flow speed ( Fig. 1-B).…”
mentioning
confidence: 99%
“…9 Additionally, transformed and nontransformed rat kidney cells, 10 malignant human breast cancer epithelial cells and benign breast epithelial cells, 11,12 and healthy and infected erythrocytes have all been shown to have different electrical properties. 13 Dielectrophoresis (DEP), the motion of a particle due to its polarization in the presence of a non-uniform electric field, 14 has been used to manipulate particles, including mixing, 15 separation, [16][17][18] enrichment, 19,20 detection, 21 and to investigate their specific electrical properties.…”
Section: Introductionmentioning
confidence: 99%
“…Along the horizontal plane, the shape and steepness of the gradient are controlled by adjusting the size and positions of the electrodes and of any insulating features in the channel that might further distort the field lines [10]. Alternatively, vertical electrodes of the same geometry on either side of a channel can be fabricated with dielectrophoretic steering forces provided by actuation of electrodes on either side of the channel with different frequencies or voltages [11], [12].…”
Section: A 3-d Electrodesmentioning
confidence: 99%