Contrast-Enhanced µCT for Visualizing and Evaluating Murine Intestinal Inflammation

Jung, Dennis; Heiß, Rafael; Kramer, Viktoria; Thoma, Oana‐Maria; Regensburger, Adrian P.; Rascher, Wolfgang; Uder, Michael; Knieling, Ferdinand; Waldner, Maximilian J.

doi:10.7150/thno.26013

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…Currently, IBD is diagnosed based on clinical symptoms, histologic criteria, and results of endoscopic and non-invasive imaging such as computed tomography (CT), [6,7] positron emission tomography (PET), and magnetic resonance imaging (MRI). [8] However, histopathological analysis cannot reflect intestinal inflammation in real-time and in situ.…”

Section: Introductionmentioning

confidence: 99%

Orally Administrable Aggregation‐Induced Emission‐Based Bionic Probe for Imaging and Ameliorating Dextran Sulfate Sodium‐Induced Inflammatory Bowel Diseases

Wang

et al. 2023

Adv Healthcare Materials

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As macrophage infiltration is significantly related to the progression of inflammatory bowel disease (IBD), monitoring the macrophages is a valuable strategy for IBD diagnosis. However, owing to the harsh physiological environment of the gastrointestinal tract and enzymatic degradation, the development of orally administrable imaging probes for tracking macrophages remains a considerable challenge. Accordingly, herein, an orally administrable aggregation‐induced emission biomimetic probe (HBTTPIP/β‐glucan particles [GPs]) is developed for tracing macrophages; HBTTPIP/GPs can diagnose and alleviate dextran sulfate sodium (DSS)‐induced colonic inflammation and self‐report the treatment efficiency. The fluorophore HBTTPIP can effectively aggregate in GPs, restricting intramolecular rotation and activating the fluorescence of HBTTPIP. After being orally administrated, HBTTPIP/GPs are phagocytosed by intestinal macrophages, which then migrate to colonic lesions, enabling non‐invasive monitoring of the severity of IBD via in vivo fluorescence imaging. Notably, oral HBTTPIP/GPs ameliorate DSS‐induced IBD by inhibiting the expressions of pro‐inflammatory factors and improving colonic mucosal barrier function. Furthermore, these HBTTPIP/GPs realize self‐feedback of the therapeutic effects of GPs on DSS‐induced colitis. The oral biomimetic probe HBTTPIP/GPs reported herein provide a novel theranostic platform for IBD, integrating non‐invasive diagnosis of IBD in situ and the corresponding treatment.

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Section: Introductionmentioning

confidence: 99%

Orally Administrable Aggregation‐Induced Emission‐Based Bionic Probe for Imaging and Ameliorating Dextran Sulfate Sodium‐Induced Inflammatory Bowel Diseases

Wang

et al. 2023

Adv Healthcare Materials

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“…[ 3 ] Until then, white light endoscopy followed by histopathology remains the reference standard to assess disease remission. [ 4 ] Advanced endoscopic optical technologies can provide cellular resolution for intestinal imaging but are limited to the mucosal surface [ 5 ] while tomographic imaging technologies such as computed tomography (CT) [ 6 ] and magnetic resonance imaging (MRI) [ 7 ] access deep‐tissue but are limited in resolution to 0.1 – 1 mm scale in patients.…”

Section: Introductionmentioning

confidence: 99%

Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis

et al. 2023

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Optoacoustic imaging (OAI) enables microscale imaging of endogenous chromophores such as hemoglobin at significantly higher penetration depths compared to other optical imaging technologies. Raster‐scanning optoacoustic mesoscopy (RSOM) has recently been shown to identify superficial microvascular changes associated with human skin pathologies. In animal models, the imaging depth afforded by RSOM can enable entirely new capabilities for noninvasive imaging of vascular structures in the gastrointestinal tract, but exact localization of intra‐abdominal organs is still elusive. Herein the development and application of a novel transrectal absorber guide for RSOM (TAG‐RSOM) is presented to enable accurate transabdominal localization and assessment of colonic vascular networks in vivo. The potential of TAG‐RSOM is demonstrated through application during mild and severe acute colitis in mice. TAG‐RSOM enables visualization of transmural vascular networks, with changes in colon wall thickness, blood volume, and OAI signal intensities corresponding to colitis‐associated inflammatory changes. These findings suggest TAG‐RSOM can provide a novel monitoring tool in preclinical IBD models, refining animal procedures and underlines the capabilities of such technologies to address inflammatory bowel diseases in humans.

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In Vivo Assessment of Deep Vascular Patterns in Murine Colitis Using Optoacoustic Mesoscopic Imaging

Buehler,

Brown,

Nedoschill

et al. 2024

Advanced Science

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The analysis of vascular morphology and functionality enables the assessment of disease activity and therapeutic effects in various pathologies. Raster‐scanning optoacoustic mesoscopy (RSOM) is an imaging modality that enables the visualization of superficial vascular networks in vivo. In murine models of colitis, deep vascular networks in the colon wall can be visualized by transrectal absorber guide raster‐scanning optoacoustic mesoscopy (TAG‐RSOM). In order to accelerate the implementation of this technology in translational studies of inflammatory bowel disease, an image‐processing pipeline for TAG‐RSOM data has been developed. Using optoacoustic data from a murine model of chemically‐induced colitis, different image segmentation methods are compared for visualization and quantification of deep vascular patterns in terms of vascular network length and complexity, blood volume, and vessel diameter. The presented image‐processing pipeline for TAG‐RSOM enables label‐free in vivo assessment of changes in the vascular network in murine colitis with broad applications for inflammatory bowel disease research.

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Contrast-Enhanced µCT for Visualizing and Evaluating Murine Intestinal Inflammation

Cited by 6 publications

References 32 publications

Orally Administrable Aggregation‐Induced Emission‐Based Bionic Probe for Imaging and Ameliorating Dextran Sulfate Sodium‐Induced Inflammatory Bowel Diseases

Orally Administrable Aggregation‐Induced Emission‐Based Bionic Probe for Imaging and Ameliorating Dextran Sulfate Sodium‐Induced Inflammatory Bowel Diseases

Transrectal Absorber Guide Raster‐Scanning Optoacoustic Mesoscopy for Label‐Free In Vivo Assessment of Colitis

In Vivo Assessment of Deep Vascular Patterns in Murine Colitis Using Optoacoustic Mesoscopic Imaging

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