Contrast enhancement with uranyl acetate allows quantitative analysis of the articular cartilage by microCT: Application to mandibular condyles in the BTX rat model of disuse

Kün‐Darbois, Jean‐Daniel; Manero, Florence; Rony, Louis; Chappard, Daniel

doi:10.1016/j.micron.2017.03.008

Cited by 17 publications

(17 citation statements)

References 25 publications

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“…The potency of various metal stains, such as osmium tetroxide, iodine, phosphotungstic acid (PTA), and gallocyanin-chromalum, to enhance contrast for micro-CT imaging has been studied 15 16 17 . Uranyl acetate has also been used as a contrasting agent for micro-CT imaging of bone and cartilage 18 19 . PTA as used in our protocol leads to consistent staining of nearly all tissues and cells in whole zebrafish specimens, yielding images that are potentially compatible with histology-like studies through full volumes of tissue.…”

Section: Introductionmentioning

confidence: 99%

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Ding

Vanselow

Katz

et al. 2018

JoVE

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For over a hundred years, the histological study of tissues has been the gold standard for medical diagnosis because histology allows all cell types in every tissue to be identified and characterized. Our laboratory is actively working to make technological advances in X-ray micro-computed tomography (micro-CT) that will bring the diagnostic power of histology to the study of full tissue volumes at cellular resolution (i.e., an X-ray Histo-tomography modality). Toward this end, we have made targeted improvements to the sample preparation pipeline. One key optimization, and the focus of the present work, is a straightforward method for rigid embedding of fixed and stained millimeter-scale samples. Many of the published methods for sample immobilization and correlative micro-CT imaging rely on placing the samples in paraffin wax, agarose, or liquids such as alcohol. Our approach extends this work with custom procedures and the design of a 3-dimensional printable apparatus to embed the samples in an acrylic resin directly into polyimide tubing, which is relatively transparent to X-rays. Herein, sample preparation procedures are described for the samples from 0.5 to 10 mm in diameter, which would be suitable for whole zebrafish larvae and juveniles, or other animals and tissue samples of similar dimensions. As proof of concept, we have embedded the specimens from Danio, Drosophila, Daphnia, and a mouse embryo; representative images from 3-dimensional scans for three of these samples are shown. Importantly, our methodology leads to multiple benefits including rigid immobilization, long-term preservation of laboriously-created resources, and the ability to re-interrogate samples.

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Section: Introductionmentioning

confidence: 99%

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Ding

Vanselow

Katz

et al. 2018

JoVE

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“…BTX injection into the masticatory muscle may influence the growth of the mandibular condyle [ 54 ]. BTX injection into the masseter muscle in mice shows the significantly reduced condylar head width [ 55 ], and the distance between the medial and lateral margins of the condylar head is also significantly reduced in growing rats [ 43 ].…”

Section: Broken Balance Muscle Function By Btx Injection and Its Ementioning

confidence: 99%

“…In BTX injection into the masseter and temporalis muscles, the bone volume is reduced in the BTX-injected side of the condyle [ 53 ]. The BTX injection into the masticatory muscle is also related to the bone density and quality of the condyle [ 53 , 54 ].…”

Section: Broken Balance Muscle Function By Btx Injection and Its Ementioning

confidence: 99%

Correction of Malocclusion by Botulinum Neurotoxin Injection into Masticatory Muscles

Seok

Kim

2018

Toxins

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Botulinum toxin (BTX) is a neurotoxin, and its injection in masticatory muscles induces muscle weakness and paralysis. This paralytic effect of BTX induces growth retardation of the maxillofacial bones, changes in dental eruption and occlusion state, and facial asymmetry. Using masticatory muscle paralysis and its effect via BTX, BTX can be used for the correction of malocclusion after orthognathic surgery and mandible fracture. The paralysis of specific masticatory muscles by BTX injection reduces the tensional force to the mandible and prevents relapse and changes in dental occlusion. BTX injection in the anterior belly of digastric and mylohyoid muscle prevents the open-bite and deep bite of dental occlusion and contributes to mandible stability after orthognathic surgery. The effect of BTX injection in masticatory muscles for maxillofacial bone growth and dental occlusion is reviewed in this article. The clinical application of BTX is also discussed for the correction of dental malocclusion and suppression of post-operative relapse after mandibular surgery.Keywords: botulinum neurotoxin; masticatory system; maxillofacial bone; dental occlusion; orthognathic surgery Key contribution: BTX injection into masticatory muscles affects the maxillofacial bony growth and dental occlusion. In clinical practices, BTX injection has been used for reducing post-operative relapse after mandibular surgery.

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“…[4][5][6][7][8][9][10][11][12][13] ). Acetate complexes of uranyl ions are used in stages of nuclear fuel reprocessing, [14,15] for staining in microcomputed tomography, [16] in gravimetric and colorimetric analysis [17] and are being widely explored, including in the sense of radioactivity and toxicity (See for example, refs. [18][19][20][21][22][23][24] ).…”

Section: Introductionmentioning

confidence: 99%

Uranyl Coordination Compounds with Alkaline Earth Metals and Crotonate Ligands

et al. 2019

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Crystal structures of three new uranyl compounds were solved using single crystal X‐ray diffraction and studied using FTIR spectroscopy. Although the syntheses conditions were very similar differing only in the alkaline earth metal (Mg, Ca or Sr), the resulting structures are different, containing, respectively, mono‐, tri‐ and hexanuclear metal‐containing building units. This fact proves the profound crystal‐chemical role of secondary metal atoms on the formation of crystal structures. Parameters of alkaline earth metal cations in the three newly synthesized compounds as well as in the earlier reported Ba‐containing analog are discussed in detail using Voronoi–Dirichlet polyhedra and the method of intersecting spheres. Differences in packing of building units in the four mentioned compounds, as well as inter‐ and intramolecular noncovalent interactions in their structures are reported. The results of analysis are consistent with the stereoatomic model of crystal structures.

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Contrast enhancement with uranyl acetate allows quantitative analysis of the articular cartilage by microCT: Application to mandibular condyles in the BTX rat model of disuse

Cited by 17 publications

References 25 publications

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Correction of Malocclusion by Botulinum Neurotoxin Injection into Masticatory Muscles

Uranyl Coordination Compounds with Alkaline Earth Metals and Crotonate Ligands

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