Contribution of Murine Double Minute 2 Genotypes to Colorectal Cancer Risk in Taiwan

Yueh, Te Cheng; Hung, Yi Wen; Shih, Tzu Ching; Wang, Shou Cheng; Lai, Yu‐Hung; Hsu, Shih Wei; Wu, Ming; Fu, Chun Kai; Wang, Yun Chi; Chang, Wen Shin; Tsai, Chia Wen; Bau, Da‐Tian

doi:10.21873/cgp.20099

Cited by 18 publications

(22 citation statements)

References 39 publications

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“…In detail, some positive findings have supported that the GT and GG genotypes at MDM2 rs2279744 are associated with higher cancer risk than the wild-type TT genotype (15)(16)(17)(18)(19)(20), whereas, other reports have shown no significant association with altered cancer risk (21)(22)(23)(24). In Taiwanese population included in the present study, the MDM2 rs2279744 homo-variant GG carriers, but not GT, were found to have an increased CRC risk compared with those carrying TT genotype (31). However, MDM2 rs2279744 genotypes were not associated with lung cancer risk (Tables II and III).…”

Section: Discussionsupporting

confidence: 60%

“…Then the DNA was quantified, stored long-term at −80˚C, diluted and aliquoted for genotyping as a working stock at −20˚C as is routinely conducted (29,30). The MDM2 genotyping working flow including the designing of the forward and reverse primer sequences, the selection of corresponding restriction endonucleases, and the polymerase chain reaction (PCR) cycling condition set-up were previously described (31). Briefly, the cycling conditions of PCR procedure were set as: one cycle at 94˚C for 5 min; followed by 35 cycles of 94˚C for 30 s, 59˚C for 30 s and 72˚C for 30 s; last, an extension at 72˚C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Association of Murine Double Minute 2 Genotypes and Lung Cancer Risk

Lin

Chang

Shen

et al. 2020

In Vivo

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Aim: The aim of this study was to evaluate the contribution of human mouse double minute 2 (MDM2) gene polymorphisms to the risk of Taiwan lung cancer. Materials and Methods: In this case-control study, the association of MDM2 rs2279744 genotypes with lung cancer risk was investigated among 358 lung cancer patients and 716 age-, gender-and smoking status-matched controls in Taiwan. Results: The percentages of MDM2 rs2279744 GT and GG genotypes were 50.0% and 27.4% in lung cancer group and 50.0% and 26.5% in control group, respectively [odds ratio (OR)=1.03 and 1.07, 95% confidence interval (CI)=0.75-1.43 and 0.75-1.53, respectively]. The analysis about allelic frequency showed that G allele at MDM2 rs2279744 conferred a non-significant increased cancer risk (OR=1.03, 95%CI=0.86-1.24). Conclusion: Polymorphisms of MDM2 rs2279744 may play a role in lung carcinogenesis. However, the studied genotypes were not shown as predictors of lung cancer susceptibility.

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Section: Discussionsupporting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

Association of Murine Double Minute 2 Genotypes and Lung Cancer Risk

Lin

Chang

Shen

et al. 2020

In Vivo

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“…Colorectal cancer (CRC) is a common malignant tumor-based disease that ranks third in morbidity and mortality worldwide. 1 According to Global Cancer Statistics, numbers of new CRC cases and deaths in 2018 approached 2 million and 900,000, respectively. The 5-year survival rate for CRC patients with early-stage disease (stages I and II), as determined based on TNM (tumornode-metastasis) criteria, is nearly 90%; conversely, the survival rate is only 5% for late-stage (stages III and IV) CRC patients.…”

Section: Introductionmentioning

confidence: 99%

BGN and COL11A1 Regulatory Network Analysis in Colorectal Cancer (CRC) Reveals That BGN Influences CRC Cell Biological Functions and Interacts with miR-6828-5p

Chen¹,

Qin

Dai³

et al. 2020

CMAR

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Purpose We explored specific expression profiles of BGN and COL11A1 genes and studied their biological functions in CRC using bioinformatics tools. Patients and Methods A total of 68 pairs of cancer and non-cancerous tissues from CRC patients were enrolled in this study. Methods we used in this articles including: qRT-PCR, Western blot analysis, ELISA, GO and KEGG regulatory network analysis, tumor infiltration, luciferase reporter-based protein and etc. Results According to The Cancer Genome Atlas (TCGA) data, BGN and COL11A1 expression levels were significantly higher in CRC patient samples than in samples from healthy controls. Moreover, levels were much higher in late-stage CRC than in early-stage disease, warranting evaluation of these genes as CRC prognostic biomarkers. Subsequently, qRT-PCR, Western blot analysis, and ELISA results obtained from analyses of CRC cells, tissues, and patient sera aligned with TCGA results. GO and KEGG regulatory network analysis revealed BGN- and COL11A1- associated genes that were functionally related to extracellular matrix (ECM) receptor pathway activation, with transcription factor genes RELA and NFKB1 positively associated with BGN expression and CEBPZ and SIRT1 with COL11A1 expression. Meanwhile, BGN and COL11A1 expression were separately and significantly correlated to tumor infiltration by six immune cell types. Additionally, kinase genes PLK1 and LYN appeared to be downstream targets of differentially expressed BGN and COL11A1 , respectively. In addition, the expression of PLK1 mRNA was down-regulated while BGN was down-regulated. Finally, BGN effects on CRC cell proliferation, cycle, apoptosis, invasion, and migration were studied using molecular biological methods, including luciferase reporter-based protein analysis, qRT-PCR, and Western blot results, which revealed that miR-6828-5p may regulate BGN expression. Conclusion We speculate that the use of BGN and COL11A1 as CRC biomarkers would improve CRC staging, while also providing several novel targets for use in the development of more effective CRC treatments.

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“…Genomic DNA from the leukocytes of each study subject was extracted using the QIAamp Blood Mini Kit (Qiagen, Valencia, CA, USA), stored for the long term at −80 °C, simultaneously diluted, and aliquoted and stored for genotyping as a working stock at −20 °C, as we have frequently performed previously [32,39,40].…”

Section: Methodsmentioning

confidence: 99%

Contribution of Inflammatory Cytokine Interleukin-18 Genotypes to Renal Cell Carcinoma

Chang

Shen

Yeh

et al. 2019

IJMS

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Interleukin-18 (IL-18) is a multi-functional immuno-mediator in the development and progression of many types of infectious and inflammatory diseases. In this study, we evaluated the contribution of IL-18 genotypes to renal cell carcinoma (RCC) in Taiwan via the genotyping of IL-18 -656 (A/C), -607 (A/C), and -137 (G/C). Moreover, we analyzed their interactions with smoking, alcohol drinking, hypertension, and diabetes status. The results showed an association of the AC and CC genotypes of IL-18 −607 with a significant decrease in the risk of RCC compared with the AA genotype (odds ratio (OR) = 0.44 and 0.35, 95% confidence interval (CI) = 0.27–0.72 and 0.18–0.66, p = 0.0008 and 0.0010, respectively). Furthermore, a significantly lower frequency of the C allele at -607 was observed in the RCC group (35.3% vs. 49.8%; OR = 0.53; 95% CI = 0.35–0.71, p = 0.0003). However, IL-18 -656 and -137 did not exhibit a likewise differential distribution of these genotypes between the control and case groups. Stratifying the population according to smoking, alcohol drinking, hypertension, and diabetes status revealed a different distribution of IL-18 -607 genotypes among non-smokers, non-drinkers, and patients without diabetes, but not among smokers, drinkers, or patients with diabetes. These findings suggest that IL-18 -607 genotypes may play a role in the etiology and progression of RCC in Taiwan and may serve as a useful biomarker for early detection.

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Contribution of Murine Double Minute 2 Genotypes to Colorectal Cancer Risk in Taiwan

Cited by 18 publications

References 39 publications

Association of Murine Double Minute 2 Genotypes and Lung Cancer Risk

Association of Murine Double Minute 2 Genotypes and Lung Cancer Risk

BGN and COL11A1 Regulatory Network Analysis in Colorectal Cancer (CRC) Reveals That BGN Influences CRC Cell Biological Functions and Interacts with miR-6828-5p

Contribution of Inflammatory Cytokine Interleukin-18 Genotypes to Renal Cell Carcinoma

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