Contribution of single genes within the unique short region of Aujeszky's disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity

Kimman, Tjeerd G.; Wind, Niels de; Oei-Lie, N.; Pol, J.M.A.; Berns, Anton; Gielkens, A. L. J.

doi:10.1099/0022-1317-73-2-243

Cited by 150 publications

(101 citation statements)

References 38 publications

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“…Besides TK, RR, and gE [23,46,48,68], inactivation of several other PrV genes has been shown to result in attenuation of the virus. In fact, inactivation of most genes whose products are nonessential for viral replication in cell culture somehow Table III.…”

Section: Attenuationmentioning

confidence: 99%

Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis - State of the art, June 1999

2000

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-Considerable progress has been made during the last years in understanding the molecular basis of protein function in pseudorabies virus (PrV), the causative agent of Aujeszky's disease (AD). Major topics have been the identification and functional characterisation of viral envelope glycoproteins and cellular virus receptors, elucidation of viral proteins involved in neurovirulence and neuropathogenesis, detection and characterisation of attenuating mutations present in and leading to successful attenuated live vaccines, and the near completion of the genomic sequence of PrV DNA. This review, which follows an article prepared for the 1993 AD symposium in Budapest, Hungary, will briefly summarise those recent developments and update the reader on the current state of the art in PrV research. Aujeszky's disease / pseudorabies virus / glycoprotein / molecular pathogenesis / tropismRésumé -Le virus de la maladie d'Aujeszky : virus et pathogenèse au niveau moléculaire -État des lieux, Juin 1999. Ces dernières années ont donné lieu à des progrès considérables pour la compréhension des bases moléculaires de la fonction des protéines du virus de la maladie d'Aujeszky (VMA). Les principaux sujets d'étude ont été l'identification et la caractérisation fonctionnelle des glycoprotéines d'enveloppe et des récepteurs cellulaires pour le virus, la mise en évidence des protéines virales impliquées dans la neurovirulence et la neuropathogenèse, la détection et la caractéri-sation de mutations atténuantes présentes et pouvant conduire à des vaccins atténués efficaces, et l'achèvement presque total de la séquence génomique de l'ADN du virus. Cette revue de la littéra-ture, qui fait suite à un article préparé pour le symposium de Budapest, Hongrie, sur la maladie d'Aujeszky (1993), va résumer ces développements récents, et faire une mise à jour, pour le lecteur, des recherches menées dans ce domaine. maladie d'Aujeszky / pseudorabies virus / glycoprotéine / pathogenèse moléculaire Vet. Res. 31 (2000) 99-115 99

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Section: Attenuationmentioning

confidence: 99%

Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis - State of the art, June 1999

2000

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“…Viral proteins known to be involved in the virulence of herpesviruses are a class of enzymes that function at the level of DNA synthesis; the thymidine kinase (TK), ribonuclease reductase, and dUTPase [14,25,91]. A protein kinase encoded in the unique short (Us) region also appears to affect virulence at least in PRV [47,48]. Deletion of these genes results in a recombinant virus with a reduced replication efficiency and attenuated phenotype.…”

Section: Genetically Engineered Herpesvirus Vaccinesmentioning

confidence: 99%

“…In better studied viruses, such as PRV or BHV-1, three glycoproteins (gE, gI, and gC) have been reported to be non-essential for viral replication in cell cultures, but implicated in important viral function such as target cell recognition and virulence [23,42,47,113]. For example, after intranasal inoculation in pigs, PRV gE deletion mutants had a reduced capability to propagate in olfactory and trigeminal pathways [56,79] and showed a reduced virulence in pigs [23,42,47,48].…”

Section: ) Non-essential Glycoprotein Genesmentioning

confidence: 99%

“…For example, after intranasal inoculation in pigs, PRV gE deletion mutants had a reduced capability to propagate in olfactory and trigeminal pathways [56,79] and showed a reduced virulence in pigs [23,42,47,48]. A PRV vaccine carrying deletions in both gE and TK was shown to be innocuous but still highly immunogenic for pigs [72,120].…”

Section: ) Non-essential Glycoprotein Genesmentioning

confidence: 99%

“…A PRV vaccine carrying deletions in both gE and TK was shown to be innocuous but still highly immunogenic for pigs [72,120]. No specific function has been assigned to gG and the PRV gG deficient mutants behave like field-type PRV in animal models as well as in cell cultures [3,47]. Herpesviruses with major deletions of these non-essential glycoprotein genes have been used as live attenuated vaccines, for example, the Norden and Bartha strains of PRV [88] and the Kentucky A strain of EHV-1 [27].…”

Section: ) Non-essential Glycoprotein Genesmentioning

confidence: 99%

See 2 more Smart Citations

Recombinant Viral Vector Vaccines for the Veterinary Use.

Yokoyama

Maeda

Mikami

1997

The Journal of Veterinary Medical Science

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ABSTRACT. Recently, genetically engineering using recombinant DNA techniques has been applied to design new viral vaccines in order to reduce some problems which present viral vaccines have. Up to now, many viruses have been investigated for development of recombinant attenuated vaccines or live viral vectors for delivery of foreign immunogenic antigens. In this review, we introduced three kind of viruses; herpesviruses, vaccinia viruses, and adenoviruses, which have best widely been studied as recombinant vaccines or delivery vaccines for the veterinary use. -KEY WORDS: recombinant polyvalent vaccine, recombinant vaccine, viral vector.

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