Contribution to the karyological knowledge of Odontites s.l. (Orobanchaceae) on the Iberian Peninsula and in Morocco

Delgado, Luis; Pinto-Carrasco, Daniel; Martín, Francisca Gallego; Rico, Enrique

doi:10.1007/s12224-015-9201-4

Cited by 4 publications

(17 citation statements)

References 9 publications

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“…Our flow cytometry results mostly agree with published genome sizes: Koutecky et al (2012) estimated mean 2C values of 1.20 and 2.35 pg for the small genome and large genome of O. vernus/vulgaris, respectively, compared to our estimates of 1.17 and 2.30 pg. As reported by Koutecky et al (2012) and Delgado et al (2015) based on chromosome counts, these two groups of individuals might represent two ploidy levels (diploids and tetraploids) within the O. vernus/O. vulgaris species complex.…”

Section: No Direct Relation Between Nuclear Genome Size and Chromosomsupporting

confidence: 57%

“…Samples named with the same number but different letters (e.g., Od40, Od40B, and Od40C) come from distinct plants but were either mounted on the same herbarium sheet (for Od18 and Od22) or belonged to the same population in the field. Because O. vernus and O. vulgaris were very difficult to distinguish morphologically (as previously noted by, e.g., Delgado et al 2015), we refer to these samples as O. vernus/vulgaris.…”

Section: Taxonomic Sampling and Plant Materialsmentioning

confidence: 99%

“…Because karyological data are missing for 11 out of the 27 Odontites species, we also aimed at obtaining additional data on chromosome counts, ploidy levels, and nuclear genome sizes within the genus. The chromosome number x p 12 was proposed as the basic, haploid chromosome number, and diploid and tetraploid karyotypes have been previously established (Snogerup 1977;Bolliger 1996;Rico et al 2008;Rico 2009;Delgado et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Reconstructing Species Relationships within the Recently Diversified Genus Odontites Ludw. (Orobanchaceae): Evidence for Extensive Reticulate Evolution

Gaudeul

Siljak‐Yakovlev

Jang

et al. 2018

International Journal of Plant Sciences

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Premise of research. Odontites is an herbaceous genus composed of 27 species, mostly distributed around the Mediterranean Basin. As a first step toward studying species diversification within this genus, our goals were to (i) test previous hypotheses about phylogenetic relationships among species; (ii) assess whether the closely related genera Bartsiella, Bornmuellerantha, Macrosyringion, and Odontitella should be included in Odontites; and (iii) detect potential events of reticulate evolution. Methodology. We estimated phylogenetic relationships based on nuclear (ITS and ETS) and chloroplast (psbA-trnH, trnC-ycf6, and trnL-trnF) DNA regions. Our sampling included 184 individuals representing 25 out of the 27 species of Odontites. We also counted chromosomes in five species and measured nuclear genome sizes in 139 individuals of 21 species. Pivotal results. We showed that Bartsiella and Bornmuellerantha should be returned to Odontites, while Odontitella was confirmed as distinct. Macrosyringion requires further investigations. Several phylogenetic incongruences were identified, suggesting two cases of hybrid speciation (in O. maroccanus and O. violaceus) and several introgression events. These events were very diverse in terms of number of individuals involved, species co-occurrence, direction of introgression, and incidence of genome doubling. We obtained new chromosome numbers for O. hispidulus (2np4xp40) and O. powellii (2np2xp20), and no relation between nuclear genome size and chromosome number was found. Conclusions. We inferred hybrid speciation and various introgression events, and polyploidy was also detected. Odontites appears as an interesting genus to study species diversification, providing a group of closely related species that display, e.g., morphological, ecological, or phenological character variation; cases of study at different temporal stages in the diversification process; and several contact zones between species.

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Section: No Direct Relation Between Nuclear Genome Size and Chromosomsupporting

confidence: 57%

Section: Taxonomic Sampling and Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reconstructing Species Relationships within the Recently Diversified Genus Odontites Ludw. (Orobanchaceae): Evidence for Extensive Reticulate Evolution

Gaudeul

Siljak‐Yakovlev

Jang

et al. 2018

International Journal of Plant Sciences

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“…Regarding the O. vernus complex, the situation is more intricate than in the case of the O. luteus complex, as seven taxa (species and subspecies; Bolliger, 1996), two ploidy levels (di-and tetraploid) and two basic chromosome numbers (x = 9 and x = 10; Delgado & al., 2015) are involved. The only study of intraspecific hybridization in this group (Snogerup, 1983) was performed using diploids (O. vernus subsp.…”

Section: Discussionmentioning

confidence: 99%

Unravelling the phylogeny of the root‐hemiparasitic genus Odontites (tribe Rhinantheae, Orobanchaceae): Evidence for five main lineages

Pinto-Carrasco

Scheunert

Heubl

et al. 2017

TAXON

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Despite the recent publication of several phylogenies focused on Rhinantheae, which has been expanded to include three Asian endemic genera, few studies so far have dealt with particular genera within the tribe. Here, we focus on Odontites and related genera because of the high morphological variability of the group and its unclear generic boundaries. Phylogenetic analyses were performed for nrDNA (ITS) and cpDNA (trnK region and rps16 intron) datasets, using Bayesian and Parsimony analyses. Our results cast doubt on the inclusion of Pterygiella and related genera within the Rhinantheae and support the paraphyly of Phtheirospermum, making it necessary to propose three new combinations to avoid it. Odontites is recircumscribed to include Bartsiella, Bornmuellerantha, and Macrosyringion, but not Odontitella. Within Odontites, five distinct lineages are identified. These are distinguishable either by morphological synapomorphies or by a combination of several character states. Most of the Odontites species are regarded as monophyletic. In the O. vernus and O. luteus complexes, some taxonomic changes are made to avoid paraphyly, which results in three new combinations. Supplementary MaterialElectronic Supplement (Appendix S1; Fig. S1) and DNA sequence alignment files are available in the Supplementary Data section of the online version of this article at

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“…; Rico, 2009) includes diploid and tetraploid individuals. The latter are probably of autopolyploid origin, as no distinct subgenomes were found in the karyotype (Delgado et al, 2015) and morphology is not intermediate between any two known diploid species. However, the hypothesis of an autopolyploid origin has not been addressed using genetic markers.…”

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confidence: 94%

Development of 14 microsatellite markers in Odontites vernus s.l. (Orobanchaceae) and cross‐amplification in related taxa

et al. 2016

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Premise of the study:Microsatellite primers were developed for the first time in the root hemiparasite herb Odontites vernus (Orobanchaceae). These markers will be useful to investigate the role of polyploidization in the evolution of this diploid-tetraploid complex, as well as the extent of gene flow between different ploidy levels.Methods and Results:Fourteen polymorphic and reproducible loci were identified and optimized from O. vernus using a microsatellite-enriched library and 454 Junior sequencing. The set of primers amplified di- to pentanucleotide repeats and showed two to 13 alleles per locus. Transferability was tested in 30 taxa (19 belonging to Odontites and 11 from eight other genera of Orobanchaceae tribe Rhinantheae).Conclusions:The results indicate the utility of the newly developed microsatellites in O. vernus and several other species, which will be useful for taxon delimitation and conservation genetics studies.

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Contribution to the karyological knowledge of Odontites s.l. (Orobanchaceae) on the Iberian Peninsula and in Morocco

Cited by 4 publications

References 9 publications

Reconstructing Species Relationships within the Recently Diversified Genus Odontites Ludw. (Orobanchaceae): Evidence for Extensive Reticulate Evolution

Reconstructing Species Relationships within the Recently Diversified Genus Odontites Ludw. (Orobanchaceae): Evidence for Extensive Reticulate Evolution

Unravelling the phylogeny of the root‐hemiparasitic genus Odontites (tribe Rhinantheae, Orobanchaceae): Evidence for five main lineages

Development of 14 microsatellite markers in Odontites vernus s.l. (Orobanchaceae) and cross‐amplification in related taxa

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