Control of Cell Surface Topography

Berlin, Richard D.; Oliver, Janet M.; Ukena, Thomas E.; Yin, H. H.

doi:10.1038/247045a0

Cited by 178 publications

(34 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…X1150. chicine treatment interrupts the relationship between membrane proteins (24). This implied structural relationship between membranes and intact microtubules prompted the experiments described presently which clearly show an effect of capping on tubulin subunit distribution.…”

Section: Resultsmentioning

confidence: 88%

“…A large number of observations have shown that microtubules are important in the structural support of cell membranes (22,23) but the significance of functional interactions between membrane components and cytoplasmic microtubules has only recently received attention (3,24). It has been shown that Con A-induced capping occurs in fibroblasts transformed by simian virus 40 (8), polymorphonuclear leukocytes (5), and lymphocytes (3,7,25) only after exposure to cold or treatment with microtubule disrupting agents.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Membrane-microtubule interactions: concanavalin A capping induced redistribution of cytoplasmic microtubules and colchicine binding proteins.

Albertini¹,

Clark²

1975

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Membrane-microtubule interactions: concanavalin A capping induced redistribution of cytoplasmic microtubules and colchicine binding proteins.

Albertini¹,

Clark²

1975

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

“…Sci. USA 75 (1978) 4483 result (25,26). This hypothesis provides an attractive mechanism for selective acquisition of host cell surface components because the area adjacent to surface-associated mycoplasmas would necessarily be enriched in mobile receptor molecules interacting with multivalent organisms.…”

Section: Discussionmentioning

confidence: 99%

“…This explanation would also provide a means of concentrating antigens that are associated with mycoplasmas on the cell surface. It has been reported that the predominant Thy-i molecule of T lymphoid cells can be "capped" and is thus laterally mobile (26). Within this context, the large quantities of Thy-i.…”

Section: Discussionmentioning

confidence: 99%

Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis.

Wise¹,

Cassell²,

Action³

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Mycoplasma hyorhinis, isolated by isopycnic centrifugation from supernatants of a persistently infected murine T lymphoblastoid cell line, demonstrated the presence of the Thy-1.1 differentiation alloantigen and H-2Kk histocompatibility antigens. The murine leukemia virus-related gp70 antigen also present on the surface of these lymphoblastoid cells was absent from mycoplasma preparations. Quantitative assessment of Thy-1.I present in preparations of M. hyorhinis revealed a specific activity eater or alto that of membrane preparations from lymphoblastoid cels, suggesting a marked accumulation of this T lymphocyte antigen by membrane-associated mycoplasmas. The accumulation of the Thy-1.1 antigen in association with purified mycoplasmas was also demonstrated in lymphoblastoid cells experimentally infected with a defined culture of M. hyorhinis.

show abstract

“…The heterogeneous organization of plasma membrane components and functions has been recognized for decades (Berlin et al, 1974) and there is growing consensus that plasma membrane heterogeneity is a critical regulator of signaling pathway activity. Thus, correlating the activities of receptors and signaling proteins and lipids with their spatial distribution and dynamics is essential to understand the regulation of cell signaling.…”

Section: Discussionmentioning

confidence: 99%

Markov Random Field Modeling of the Spatial Distribution of Proteins on Cell Membranes

Zhang¹

2007

Bull. Math. Biol.

View full text Add to dashboard Cite

Cell membranes display a range of receptors that bind ligands and activate signaling pathways. Signaling is characterized by dramatic changes in membrane molecular topography, including the co-clustering of receptors with signaling molecules and the segregation of other signaling molecules away from receptors. Electron microscopy of immunogold-labeled membranes is a critical technique to generate topographical information at the 5-10 nm resolution needed to understand how signaling complexes assemble and function. However, due to experimental limitations, only two molecular species can usually be labeled at a time. A formidable challenge is to integrate experimental data across multiple experiments where there are from 10 to 100 different proteins and lipids of interest and only the positions of two species can be observed simultaneously. As a solution, we propose the use of Markov random field (MRF) modeling to reconstruct the distribution of multiple cell membrane constituents from pair-wise data sets. MRFs are a powerful mathematical formalism for modeling correlations between states associated with neighboring sites in spatial lattices. The presence or absence of a protein of a specific type at a point on the cell membrane is a state. Since only two protein types can be observed, i.e., those bound to particles, and the rest cannot be observed, the problem is one of deducing the conditional distribution of a MRF with unobservable (hidden) states. Here, we develop a multiscale MRF model and use mathematical programming techniques to infer the conditional distribution of a MRF for proteins of three types from observations showing the spatial relationships between only two types. Application to synthesized data shows that the spatial distributions of three proteins can be reliably estimated. Application to experimental data provides the first maps of the spatial relationship between groups of three different signaling molecules. The work is an important step toward a more complete understanding of membrane spatial organization and dynamics during signaling.

show abstract

Control of Cell Surface Topography

Cited by 178 publications

References 7 publications

Membrane-microtubule interactions: concanavalin A capping induced redistribution of cytoplasmic microtubules and colchicine binding proteins.

Membrane-microtubule interactions: concanavalin A capping induced redistribution of cytoplasmic microtubules and colchicine binding proteins.

Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis.

Markov Random Field Modeling of the Spatial Distribution of Proteins on Cell Membranes

Contact Info

Product

Resources

About