Control of the surface properties of micro-fluidic devices for external power-free biochip application

Jang, Kyungmin; An, Yoojin; Park, Hyun; Kim, Young-Gun; Yang, Sung Yun

doi:10.1007/s13233-013-1070-1

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Cytochrome c was partially retained. As mall piece of the NCF film could be easily bonded across the microfluidic channel; [23] the molecular separation performances from the aqueous solutions (Figure 3d)were observed to be similar to those observed using the dead-end stirred cell. Theh ydrophilicity and the chemical and mechanical robustness of the NCF film make it suitable as am odular membrane for microfluidic devices.…”

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confidence: 53%

Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes

et al. 2016

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The preparation of bicontinuous nanoporous covalent frameworks, which are promising for caging active enzymes, is demonstrated. The frameworks have three- dimensionally continuous, hydrophilic pores with widths varying between 5 and 30 nm. Enzymes were infiltrated into the bicontinuous pore by applying a pressured enzyme solution. The new materials and methods allowed the amount of caged proteins to be controlled precisely. The resulting enzyme-loaded framework films could be recycled many times with nearly no loss of catalytic activity. Entropic trapping of proteins by a bicontinuous pore with the right size distribution is an unprecedented strategy toward facile in vitro utilization of biocatalysts.

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confidence: 53%

Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes

et al. 2016

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“…A novel microfluidic system that models the tumor microenvironment observed in vivo has also been fabricated [ 13 ]. Jang et al utilized polyelectrolyte multilayer coatings to modify the surface of the microchannel of a microfluidic device to control cellular interactions inside of the channel [ 14 ]. A microfluidic device incorporating multifunctional composite materials also became very attractive to develop new delivery system [ 15 , 16 ].…”

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confidence: 99%

RETRACTED ARTICLE: Fabrication of a microfluidic device for studying the in situ drug-loading/release behavior of graphene oxide-encapsulated hydrogel beads

2018

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BackgroundControlled drug delivery system is highly important for not only prolonged the efficacy of drug but also cellular development for tissue engineering. A number of biopolymer composites and nanostructured carriers behave been used for the controlled drug delivery of therapeutics. Recently, in vitro microfluidic devices that mimic the human body have been developed for drug-delivery applications.MethodsA microfluidic channel was fabricated via a two-step process: (i) polydimethyl siloxane (PDMS) and curing agent were poured with a 10:2 mass ratio onto an acrylic mold with two steel pipes, and (ii) calcium alginate beads were synthesized using sodium alginate and calcium chloride solutions. Different amounts (10, 25, 50 μg) of graphene oxide (GO) were then added by Hummers method, and studies on the encapsulation and release of the model drug, risedronate (Ris), were performed using control hydrogel beads (pH 6.3), GO-containing beads (10GO, 25GO and 50GO), and different pH conditions. MC3T3 osteoblastic cells were cultured in a microchannel with Ris-loaded GO-hydrogel beads, and their proliferation, viability, attachment and spreading were assessed for a week.ResultsThe spongy and textured morphology of pristine hydrogel beads was converted to flowery and rod-shaped structures in drug-loaded hydrogel beads at reduced pH (6.3) and at a lower concentration (10 μg) of GO. These latter 10GO drug-loaded beads rapidly released their cargo owing to the calcium phosphate deposited on the surface. Notably, beads containing a higher amount of GO (50GO) exhibited an extended drug-release profile. We further found that MC3T3 cells proliferated continuously in vitro in the microfluidic channel containing the GO-hydrogel system. MTT and live/dead assays showed similar proliferative potential of MC3T3 cells. Therefore, a microfluidic device with microchannels containing hydrogel beads formulated with different amounts of GO and tested under various pH conditions could be a promising system for controlled drug release.ConclusionsThe GO and drug (risedronate, Rig) were directed loaded into a hydrogel placed in a microchannel. Through interactions such as hydrogen bonding between Go and the Rig-loaded GO-hydrogel beads, the bead-loaded microfluidic device supported MC3T3 proliferation and development as osteoblast without additional osteogenic differentiation supplements.

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Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes

et al. 2016

Self Cite

View full text Add to dashboard Cite

The preparation of bicontinuous nanoporous covalent frameworks, which are promising for caging active enzymes, is demonstrated. The frameworks have three‐ dimensionally continuous, hydrophilic pores with widths varying between 5 and 30 nm. Enzymes were infiltrated into the bicontinuous pore by applying a pressured enzyme solution. The new materials and methods allowed the amount of caged proteins to be controlled precisely. The resulting enzyme‐loaded framework films could be recycled many times with nearly no loss of catalytic activity. Entropic trapping of proteins by a bicontinuous pore with the right size distribution is an unprecedented strategy toward facile in vitro utilization of biocatalysts.

show abstract

Control of the surface properties of micro-fluidic devices for external power-free biochip application

Cited by 5 publications

References 16 publications

Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes

Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes

RETRACTED ARTICLE: Fabrication of a microfluidic device for studying the in situ drug-loading/release behavior of graphene oxide-encapsulated hydrogel beads

Bicontinuous Nanoporous Frameworks: Caged Longevity for Enzymes

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