Controlled and Sequential Delivery of Stromal Derived Factor-1 α (SDF-1α) and Magnesium Ions from Bifunctional Hydrogel for Bone Regeneration

Abstract

Bone healing is a complex process that requires the participation of cells and bioactive factors. Stromal derived factor-1 α (SDF-1α) and magnesium ions (Mg2+) both are significant bioactive factors for cell recruitment and osteogenesis during bone regeneration. Thus, a bifunctional hydrogel containing a sequential delivery system is fabricated to improve osteogenesis. During sequential delivery of the hydrogel, SDF-1α is predominantly released at the early stage of bone mesenchymal stem cells (BMSCs) recruitment, while Mg2+ are constantly delivered at a later stage to improve osteogenic differentiation of recruited cells. In addition, due to the early release of SDF-1α, the hydrogel showed strong BMSCs recruitment and proliferation activity. Mg2+ can not only induce up-regulation of osteogenic gene expression in vitro, but also promote bone tissue and angiogenesis in vivo. Taken together, the injection of xanthan gum-polydopamine crosslinked hydrogel co-loading SDF-1α and Mg2+ (XPMS hydrogel) provides a novel strategy to repair bone defects.

The Antagonism of Neuropeptide Y Type I Receptor (Y1R) Reserves the Viability of Bone Marrow Stromal Cells in the Milieu of Osteonecrosis of Femoral Head (ONFH)

“…Stromal cell-derived factor-1 (SDF-1) is a chemokine that could facilitate the survival and osteogenic differentiation of BMSCs [ 20 ]. We employed an RT-PCR assay to evaluate the transcription level of SDF-1 under serial concentrations of Y1R agonists and antagonists.…”

Reviving Intervertebral Discs: Treating Degeneration Using Advanced Delivery Systems

“…However, SDF-1α is easily degraded by MMP, which may hinder its practical application. Therefore, designing reasonable biomaterials to deliver SDF-1α to continuously recruit MSCs and promote IVD regeneration is a feasible solution, and researchers have made many attempts in this regard. , In previous studies, the delivery of this chemokine using a thermosensitive hydrogel as a carrier has been shown to significantly increase the number of MSCs migrating to the IVD . However, the release of SDF-1α from the hydrogel was relatively slow, which resulted in a longer time being required to recruit high chemotactic gradient MSCs.…”

Advances and Trends of Photoresponsive Hydrogels for Bone Tissue Engineering

“…Therefore, delivering multiple biomolecules correlated with bone healing to augment bone remodeling would be preferable. Recently, codelivery systems consisting of various growth factors and drugs have been extensively studied for BTE. − However, the release of multiple biomolecules primarily occurs simultaneously, making it uncertain regarding their release time and concentration in the body. For instance, Subbiah et al designed a drug delivery system to investigate the codelivery of dual growth factors.…”